Recombinant Human Dkk-1 Protein, CF

Catalog #: 11387-DK Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
11387-DK-020
11387-DK-050
11387-DK-100
11387-DK-250
11387-DK-01M

Best Seller

Recombinant Human Dkk‑1 Protein Bioactivity.

2 Images

Product Details

Reviews

Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Human Dkk-1 Protein, CF Summary

Product Specifications

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

Activity

Measured by its ability to inhibit Wnt induced TCF reporter activity in HEK293 human embryonic kidney cells. The ED₅₀ for this effect is 8.00-96.0 ng/mL.

Source

Human embryonic kidney cell, HEK293-derived human Dkk-1 protein
Thr32-His266

Accession #

O94907.1

N-terminal Sequence
Analysis

Thr32

Predicted Molecular Mass

26 kDa

SDS-PAGE

38-42 kDa, under reducing conditions.

Product Datasheets

11387-DK

Product Datasheet

COA

SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

11387-DK

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution	Reconstitute the 20 μg size at 200 μg/mL in PBS. Reconstitute all other sizes at 500 μg/mL in PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Scientific Data

Bioactivity

View Larger

Recombinant Human Dkk-1 (Catalog # 11387-DK) inhibits Recombinant Mouse Wnt-3a (1324-WN) induced TCF reporter activity in HEK293 human embryonic kidney cells. The ED₅₀ for this effect is 8.00‑96.0 ng/mL.

SDS-PAGE

View Larger

2 μg/lane of Recombinant Human Dkk‑1 Protein (Catalog # 11387-DK) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 38-42 kDa, under reducing conditions.

Reconstitution Calculator

Background: Dkk-1

Dickkopf related protein 1 (Dkk-1) is the founding member of the Dickkopf family of proteins that includes Dkk-1, -2, -3, -4, and a related protein, Soggy (1, 2). Dkk proteins are secreted proteins that contain two conserved cysteine-rich domains separated by a linker region. Each domain contains ten cysteine residues (1‑3). Mature human Dkk-1 is a 40 kDa glycosylated protein that shares 86%, 87%, 90% and 91% aa sequence identity with mouse, rat, rabbit and bovine Dkk-1, respectively. It also shares 42% and 36% aa identity with human Dkk-2 and Dkk-4, respectively. Dkk-1 and Dkk-4 are well documented antagonists of the canonical Wnt signaling pathway (1, 2). This pathway is activated by Wnt engagement of a receptor complex composed of the Frizzled proteins and one of two low-density lipoprotein receptor-related proteins, LRP5 or LRP6 (4). Dkk-1 antagonizes Wnt by forming ternary complexes of LRP5/6 with Kremen1 or Kremen2 (4, 5). Dkk‑1/LRP6/Krm2 complex internalization has been shown to down-regulate Wnt signaling (4, 5). Dkk-1 is expressed throughout development and antagonizes Wnt-7a during limb development (6, 7). Other sites of expression include developing neurons, hair follicles and the retina of the eye (8, 9). The balance between Wnt signaling and Dkk-1 inhibition is critical for bone formation and homeostasis (10). Insufficient or excess Dkk-1 activity in bone results in increased or decreased bone density, respectively (8, 11). In adults, Dkk-1 is expressed in osteoblasts and osteocytes, and neurons. Cerebral ischemia induces Dkk-1 expression, which contributes to neuronal cell death (12).

References

Krupnik, V.E. et al. (1999) Gene 238:301.
Niehrs, C. (2006) Oncogene 25:7469.
Bullock, C.M. et al. (2004) Mol. Pharmacol. 65:582.
Mao, B. et al. (2001) Nature 411:321.
Mao, B. et al. (2002) Nature 417:664.
Kemp, C. et al. (2005) Dev. Dyn. 233:1064.
Adamska, M. et al. (2004) Dev. Biol. 272:134.
Li, J. et al. (2006) Bone 36:754.
Verani, R. et al. (2006) J. Neurochem. 101:242.
Pinzone, J.J. et al. (2009) Blood 113:517.
Morvan, F. et al. (2006) J. Bone Miner. Res. 21:934.
Cappuccio, I. et al. (2005) J. Neurosci. 25:2647.

Long Name

Dickkopf-1

Entrez Gene IDs

22943 (Human); 13380 (Mouse); 102123735 (Cynomolgus Monkey)

Alternate Names

dickkopf (Xenopus laevis) homolog 1; dickkopf homolog 1 (Xenopus laevis); dickkopf related protein-1; Dickkopf-1; dickkopf-related protein 1; Dkk1; Dkk-1; hDkk-1; SKdickkopf-1 like