Recombinant Human E-Cadherin Protein, CF

Name: Recombinant Human E-Cadherin Protein, CF
Brand: R&D Systems
SKU: 8505-EC
Rating: 5 (1 reviews)

Catalog #: 8505-EC
9 Citations 1 Review Datasheet / COA / SDS

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8505-EC-050

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Citations (9)

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Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Human E-Cadherin Protein, CF Summary

Product Specifications

Purity

>95%, by SDS-PAGE with silver staining

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

Activity

Measured by the ability of the immobilized protein to support the adhesion of the MCF‑7 human breast cancer cells. The ED₅₀ for this effect is 0.2-1 μg/mL

Source

Human embryonic kidney cell, HEK293-derived human E-Cadherin protein
Asp155-Ile707, with a C-terminal 6-His tag

Accession #

P12830

N-terminal Sequence
Analysis

Asp155

Structure / Form

Noncovalently-linked homodimer

Predicted Molecular Mass

61 kDa

SDS-PAGE

74-92 kDa, reducing conditions

Product Datasheets

8505-EC

Product Datasheet

COA

SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

8505-EC

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution	Reconstitute at 250 μg/mL in sterile PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Reconstitution Calculator

Background: E-Cadherin

E-Cadherin/Cadherin-1, also known as Uvomorulin in the mouse and rat, is a 120 kDa member of the Cadherin family of cell surface glycoproteins that mediate cell adhesion (1). Human E-Cadherin shares 81% amino acid sequence identity with the rat and mouse proteins (2). It is a single-pass transmembrane protein that mediates calcium-dependent epithelial cell adhesion. E-Cadherin has five extracellular EC domains that form homophilic cis-clusters between adjacent epithelial cells and trans-clusters within the same cell. E-Cadherin clusters are critical components of adherens junctions between epithelial cells and act in the formation and maintenance of the epithelial cell barrier (3, 4). The intracellular domain of E-Cadherin binds to the Catenin cytoskeletal complex, which includes p120 Catenin, beta-Catenin, alpha-Catenin, and Vinculin. E-Cadherin expression is critical for epithelial tissue homeostasis. Decreased E-Cadherin is associated with physiological and pathological epithelial-to-mesenchymal transition and cell migration, and E-Cadherin loss contributes to cancer metastasis (5). The extracellular E-Cadherin N-terminal domain can be cleaved by several proteases and is released as a soluble factor that enhances cancer cell motility and EGFR-dependent survival and proliferation (6).

References

Gumbiner, B.M. (2005) Nat. Rev. Mol. Cell Biol. 6:622.
Bussemakers, M.J. et al. (1993) Mol. Biol. Rep. 17:123.
Guillot, C. and T. Lecuit (2013) Science 340:1185.
Tian, X. et al. (2011) J. Biomed. Biotechnol. 2011:567305.
Stemmler, M.P. (2008) Mol. Biosyst. 4:835.
David, J.M. and A.K. Rajasekaran (2012) Cancer Res. 72:2917.

Entrez Gene IDs

999 (Human); 12550 (Mouse); 83502 (Rat)

Alternate Names

Arc-1; CAD1; cadherin 1, E-cadherin (epithelial); cadherin 1, type 1, E-cadherin (epithelial); Cadherin-1; calcium-dependent adhesion protein, epithelial; CAM 120/80; CD324 antigen; CD324; CDH1; CDHE; cell-CAM 120/80; Cell-CAM120/80; ECAD; ECadherin; E-Cadherin; Epithelial cadherin; LCAM; L-CAM; UVOE-Cadherin; Uvomorulin

Related Research Areas

Citations for Recombinant Human E-Cadherin Protein, CF

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

9 Citations: Showing 1 - 9
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The matrix metalloproteinase ADAM10 supports hepatitis C virus entry and cell-to-cell spread via its sheddase activity
Authors: Carriquí-Madroñal, B;Sheldon, J;Duven, M;Stegmann, C;Cirksena, K;Wyler, E;Zapatero-Belinchón, FJ;Vondran, FWR;Gerold, G;
PLoS pathogens
Species: Human
Sample Types: Whole Cells
Applications: Bioassay
Truncated vitronectin with E-cadherin enables the xeno-free derivation of human embryonic stem cells
Authors: Souralova, T;Hulinova, D;Jeseta, M;Ventruba, P;Hampl, A;Koutna, I;
Scientific reports
Species: Human
Sample Types: Embryo
Applications: Bioassay
HtrA is involved in stress response and adhesion in Glaesserella parasuis serovar 5 strain Nagasaki
Authors: X Zhang, Y Lin, X Xu, S Wen, Z Wang, J Gu, Q He, X Cai
Veterinary Microbiology, 2023-04-18;282(0):109748.
Species: N/A
Sample Types: Recombinant Protein
Applications: Bioassay
Functional assessment of donated human embryos for the generation of pluripotent embryonic stem cell lines
Authors: II Ceschin, AP Ceschin, MS Joya, TG Mitsugi, LK Nishikawa, AC Krepischi, OK Okamoto
Reproductive biomedicine online, 2022-12-02;0(0):.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay
Adherens junctions organize size-selective proteolytic hotspots critical for Notch signalling
Authors: M Kwak, KM Southard, WR Kim, A Lin, NH Kim, R Gopalappa, HJ Lee, M An, SH Choi, Y Jung, K Noh, J Farlow, A Georgakopo, NK Robakis, MK Kang, ML Kutys, D Seo, HH Kim, YH Kim, J Cheon, ZJ Gartner, YW Jun
Nature Cell Biology, 2022-12-01;24(12):1739-1753.
Species: Human
Sample Types: Transfected Whole Cells
Applications: Bioassay
Targeting breast and pancreatic cancer metastasis using a dual-cadherin antibody
Authors: DS Micalizzi, D Che, BT Nicholson, JF Edd, N Desai, ER Lang, M Toner, S Maheswaran, DT Ting, DA Haber
Proceedings of the National Academy of Sciences of the United States of America, 2022-10-18;119(43):e2209563119.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot Control
A bacterial metabolite ameliorates periodontal pathogen-induced gingival epithelial barrier disruption via GPR40 signaling
Authors: M Yamada, N Takahashi, Y Matsuda, K Sato, M Yokoji, B Sulijaya, T Maekawa, T Ushiki, Y Mikami, M Hayatsu, Y Mizutani, S Kishino, J Ogawa, M Arita, K Tabeta, T Maeda, K Yamazaki
Sci Rep, 2018-06-13;8(1):9008.
Species: Bacteria
Sample Types: Bacteria
Applications: Bioassay
Bimodal sensing of guidance cues in mechanically distinct microenvironments
Authors: ED Tabdanov, VV Puram, Z Win, A Alamgir, PW Alford, PP Provenzano
Nat Commun, 2018;9(1):4891.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay
Cadherin 26 is an alpha integrin-binding epithelial receptor regulated during allergic inflammation
Authors: JM Caldwell, MH Collins, KA Kemme, JD Sherrill, T Wen, M Rochman, EM Stucke, L Amin, H Tai, PE Putnam, MJ Jim‚nez-Da, MR Wormald, A Porollo, JP Abonia, ME Rothenberg
Mucosal Immunol, 2017-01-04;0(0):.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay