Recombinant Human ErbB2/Her2 Fc Chimera Avi-tag Protein, CF

Catalog #: AVI1129
1 Citation Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AVI1129-050

Recombinant Human ErbB2/Her2 Fc Chimera Avi-tag Protein Binding Activity

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Product Details

Citations (1)

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Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Human ErbB2/Her2 Fc Chimera Avi-tag Protein, CF Summary

Learn more about Avi-tag Biotinylated Proteins

Product Specifications

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

Activity

Measured by its binding ability in a functional ELISA. When Human ErbB2/Her2 (Trastuzumab) Antibody (Catalog # MAB9589) is immobilized at 0.5 µg/mL (100 µL/well), Biotinylated Recombinant Human ErbB2/Her2 Fc Chimera Avi-tag binds with an ED₅₀ of 10-60 ng/mL.

Source

Human embryonic kidney cell, HEK293-derived human ErbB2/Her2 protein

Human ErbB2/Her2 (Ser22-Thr652) Accession # P04626-1	IEGRMD	Human IgG₁ (Pro100-Lys330)	Avi-tag
N-terminus			C-terminus

Accession #

P04626-1

N-terminal Sequence
Analysis

Ser22 & Thr23

Structure / Form

Disulfide-linked homodimer, biotinylated via Avi-tag

Predicted Molecular Mass

98 kDa

SDS-PAGE

120-138 kDa, under reducing conditions

Product Datasheets

AVI1129

Product Datasheet

COA

SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

AVI1129

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution	Reconstitute at 500 μg/mL in PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Scientific Data

Binding Activity

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When Human ErbB2/Her2 (Trastuzumab) Antibody (Catalog # MAB9589) is immobilized at 0.5 µg/mL (100 µL/well), Biotinylated Recombinant Human ErbB2/Her2 Fc Chimera Avi-tag (Catalog # AVI1129) binds with an ED₅₀ of 10-60 ng/mL.

SDS-PAGE

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2 μg/lane of Biotinylated Recombinant Human ErbB2/Her2 Fc Chimera Avi-tag (Catalog # AVI1129) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® blue staining, showing bands at 120-138 kDa and 240-280 kDa, respectively.

Reconstitution Calculator

Background: ErbB2/Her2

ErbB2, also called Neu and Her2 (human epidermal growth factor receptor 2), is a type I membrane glycoprotein that is a member of the ErbB family of tyrosine kinase receptors. ErbB family members serve as receptors for the epidermal growth factor (EGF) family of growth factors. ErbB2 is widely expressed in epithelial cells and has also been found to be over-expressed in a large number of breast carcinomas. Among ErbB family members, ErbB2 is unique in that it has no identified ligands. Rather, ErbB2 heterodimerizes with the other members of the ErbB family (ErbB1 (EGFR), ErbB3, ErbB4) to form higher affinity signaling complexes. Because ErbB3 contains a defective kinase domain, the kinase domain of ErbB2 is responsible for initiating the tyrosine phosphorylation signal through the heterodimeric receptor. It has been found that a discrete three amino acid signal in the ErbB3 cytoplasmic domain is critical for transactivation of ErbB2. Interestingly, this same three amino acid signal has also been found in ErbB1 and ErbB4. Phosphoinositide 3-kinase has been shown to play a role in ErbB2 signal transduction. The cytoplasmic domain of ErbB2 has been shown to associate with beta-catenin and plakoglobin. Human ErbB2 consists of 1255 amino acids (aa) with a 21 aa signal sequence, a 631 aa extracellular domain, a 23 aa transmembrane region, and a 580 aa cytoplasmic domain. ErbB2 can be shed from the cell surface by proteolytic cleavage by an unidentified protease. ErbB2 appears to play roles in development, cancer, communication at the neuromuscular junction and regulation of cell growth and differentiation (1-10).

References

Coussens, L. et. al. (1985) Science 230:1132.
Yamamoto, T. et. al. (1986) Nature 319:230.
Kanai, Y. et. al. (1995) Biochem. Biophys. Res. Commun. 208:1067.
Codony-Servat, J. et. al. (1999) Cancer Res. 59:1196.
Carraway, K.L. 3^rd et. al. (1994) J. Biol. Chem. 269:14303.
Emkey, R. and C.R. Kahn (1997) J. Biol. Chem. 272:31172.
Schaefer, G. et. al. (1999) J. Biol. Chem. 274:859.
Schlessinger, J. (2000) Cell 103:211.
Hellyer, N.J. et. al. (2001) J. Biol. Chem. 276:42153.
Daly, R.J. (1999) Growth Factors 16:255.

Long Name

Receptor Tyrosine Protein Kinase ErbB2

Entrez Gene IDs

2064 (Human); 13866 (Mouse); 24337 (Rat); 102146608 (Cynomolgus Monkey)

Alternate Names

CD340 antigen; CD340; c-erb B2/neu protein; EC 2.7.10; EGFR2; ErbB2; HER2; HER-2; HER2EC 2.7.10.1; herstatin; Metastatic lymph node gene 19 protein; MLN 19; MLN19; Neu Oncogene; NEUHER-2/neu; neuroblastoma/glioblastoma derived oncogene homolog; NGL; NGLTKR1; p185erbB2; Proto-oncogene c-ErbB-2; Proto-oncogene Neu; receptor tyrosine-protein kinase erbB-2; TKR1; Tyrosine kinase-type cell surface receptor HER2; v-erb-b2 avian erythroblastic leukemia viral oncogene homolog 2(neuro/glioblastoma derived oncogene homolog); v-erb-b2 erythroblastic leukemia viral oncogene homolog 2, neuro/glioblastomaderived oncogene homolog (avian)

Related Research Areas

Citation for Recombinant Human ErbB2/Her2 Fc Chimera Avi-tag Protein, CF

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

Effectiveness of 4-1BB-costimulated HER2-targeted chimeric antigen receptor T cell therapy for synovial sarcoma
Authors: Y Murayama, H Kawashima, N Kubo, C Shin, Y Kasahara, M Imamura, N Oike, T Ariizumi, A Saitoh, K Mihara, H Umezu, A Ogose, C Imai
Translational Oncology, 2021-09-21;14(12):101227.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry