Recombinant Human Fc gamma RIIIA/CD16a His Avi Protein, CF

Catalog #: AVI10468 Datasheet / COA / SDS

Biotinylated Isoform V176

Catalog #	Availability	Size / Price	Qty
AVI10468-050

Recombinant Human Fc gamma RIIIA/CD16a His Avi Protein Binding Activity

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All Fc gamma RIIIA/CD16a Proteins and Enzymes

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Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Human Fc gamma RIIIA/CD16a His Avi Protein, CF Summary

Learn more about Avi-tag Biotinylated Proteins

Product Specifications

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

Activity

The biotin to protein ratio is greater than 0.7 as determined by the HABA assay. Measured by its binding ability in a functional ELISA. When Human IgG is immobilized at 2 μg/mL, 100 μL/well, it binds Biotinylated Recombinant Human Fc gamma RIIIA/CD16a His-tag Avi-tag (Catalog # AVI10468) with an ED₅₀ of 4-40 ng/mL.

Source

Human embryonic kidney cell, HEK293-derived human Fc gamma RIIIA/CD16a protein

Human Fc gamma RIIIA/CD16a (Gly17-Gln208) Accession # AAH17865.1	6-His tag	Avi-tag
N-terminus		C-terminus

Accession #

AAH17865.1

N-terminal Sequence
Analysis

Gly17

Structure / Form

Biotinylated via Avi-tag

Predicted Molecular Mass

24 kDa

SDS-PAGE

48-57 kDa, under reducing conditions

Product Datasheets

AVI10468

Product Datasheet

COA

SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

AVI10468

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution	Reconstitute at 500 μg/mL in PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Scientific Data

Binding Activity

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When Human IgG is immobilized at 2 μg/mL, 100 μL/well, it binds Biotinylated Recombinant Human Fc gamma RIIIA/CD16a His-tag Avi-tag (AVI10468) with an ED₅₀ of 4-40 ng/mL.

SDS-PAGE

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2 μg/lane of Biotinylated Recombinant Human Fc gamma RIIIA/CD16a His-tag Avi-tag (AVI10468) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 48-57 kDa.

Reconstitution Calculator

Background: Fc gamma RIIIA/CD16a

Fc gamma RIIIA/CD16a is a low/intermediate affinity receptor for polyvalent immune-complexed IgG. It is involved in phagocytosis, secretion of enzymes and inflammatory mediators, antibody-dependent cytotoxicity, and clearance of immune complexes (1-3). In humans, it is expressed as a 50-70 kDa transmembrane activating receptor on NK cells, T cells, monocytes, and macrophages (2). It is closely related to the GPI-linked Fc gamma RIIIB which is expressed on human neutrophils and eosinophils (1, 3). These two proteins share 97% amino acid (aa) identity within their extracellular domains (ECD) (4). The ECD of Fc gamma RIIIA also shares 63%, 61%, 65%, 59%, and 58% aa identity with mouse Fc gamma RIV, rat Fc gamma RIIIA, feline CD16, bovine CD16, and porcine Fc gamma RIIIB, respectively. Mature human Fc gamma RIIIA consists of a 192 aa ECD with two C2-type Ig-like domains, a 21 aa transmembrane segment, and a 25 aa cytoplasmic domain. In humans, a single nucleotide polymorphism (T230A) creates high binding (176V) and low binding (176F) forms that may influence susceptibility to autoimmune diseases or response to therapeutic IgG antibodies (5, 6). Fc gamma RIIIA surface expression requires interaction with an accessory chain, either the common gamma -chain or CD3 zeta (7, 8). Glycosylation patterns, electrophoretic mobility, and binding affinity appear to differ between NK cell and monocyte Fc gamma RIIIA (9). Shed forms of both Fc gamma RIIIA and Fc gamma RIIIB can be generated by proteolytic cleavage and retain binding activity (10-13). Shedding from monocytes and macrophages can be triggered by cell activation or phagocytosis (13). Soluble Fc gamma RIII circulates in normal plasma and is elevated in rheumatoid arthritis and in coronary artery diseases (11, 12). Our Avi-tag Biotinylated human Fc gamma RIIIA features biotinylation at a single site contained within the Avi-tag, a unique 15 aa peptide. Protein orientation will be uniform when bound to streptavidin-coated surface due to the precise control of biotinylation and the rest of the protein is unchanged so there is no interference in the protein's bioactivity.

References

Nagelkerke, S.Q. and T.W. Kuijpers (2015) Front. Immunol. 5:674.
Nimmerjahn, F. and J.V. Ravetch (2006) Immunity 24:19.
Ravetch, J.V. and B. Perussia (1989) J. Exp. Med. 170:481.
Scallon, B.J. et al. (1989) Proc. Natl. Acad. Sci. USA 86:5079.
Wu, J. et al. (1997) J. Clin. Invest. 100:1059.
Dall’Ozzo, S. et al. (2004) Cancer Res. 64:4664.
Kim, M.-K. et al. (2003) Blood 101:4479.
Lanier, L.L. et al. (1989) Nature 342:803.
Edberg, J.C. and R.P. Kimberley (1997) J. Immunol. 159:3849.
Li, P. et al. (2007) J. Biol. Chem. 282:6210.
Masuda, M. et al. (2003) J. Rheumatol. 30:1911.
Masuda, M. et al. (2006) Atherosclerosis 188:377.
Webster, N.L. et al. (2006) J. Leukoc. Biol. 79:294.

Long Name

Fc gamma Receptor III A

Entrez Gene IDs

2214 (Human); 304966 (Rat)

Alternate Names

CD16a antigen; CD16a; CD16FCRIIIA; Fc fragment of IgG, low affinity IIIa, receptor (CD16a); Fc fragment of IgG, low affinity IIIa, receptor for (CD16); Fc gamma receptor III-A; Fc gamma RIIIA; FCG3; Fc-gamma receptor III-2 (CD 16); Fc-gamma receptor IIIb (CD16); Fc-gamma RIII; Fc-gamma RIIIa; Fc-gamma RIII-alpha; FCGR3; FCGR3A; FCGRIII; FcgRIIIA; FcR-10; FCRIII; FcRIIIa; IGFR3FcgammaRIIIA; immunoglobulin G Fc receptor III; low affinity III, receptor for (CD16); low affinity immunoglobulin gamma Fc region receptor III-A; neutrophil-specific antigen NA