Recombinant Human Fc gamma RIIIA (F176) His Avi Protein, CF

Catalog #: AVI8894 Datasheet / COA / SDS

Biotinylated

Catalog #	Availability	Size / Price	Qty
AVI8894-050

Recombinant Human Fc gamma RIIIA (F176) His Avi Protein Binding Activity

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All Fc gamma RIIIA/CD16a Proteins and Enzymes

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Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Human Fc gamma RIIIA (F176) His Avi Protein, CF Summary

Learn more about Avi-tag Biotinylated Proteins

Why choose R&D Systems Avi-tag Fc gamma RIIIA Protein?

Guaranteed Bioactivity and High Purity: Bioactivity tested by functional ELISA and purity determined by SDS-PAGE to be greater than 95%.
Lot-to-Lot Consistency: Stringent QC testing performed on each lot to ensure consistent activity and purity.
Bulk Quantities Available: Bulk up and save with large mass quantities to meet your research needs. Supply agreements available, partner with us. Please contact us.
Most Respected, Most Cited Brand in Proteins: With over 35 years of providing the best recombinant proteins to the scientific community, R&D Systems continues to lead the industry in quality, activity, and purity.

Product Specifications

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

Activity

The biotin to protein ratio is greater than 0.7 as determined by the HABA assay. Measured by its binding ability in a functional ELISA. When Human IgG is immobilized at 5 µg/mL (100 µL/well), Biotinylated Recombinant Human Fc gamma RIIIA/CD16a (F176) His-tag Avi-tag (Catalog # AVI8894) binds with an ED₅₀ of 50-300 ng/mL.

Source

Human embryonic kidney cell, HEK293-derived human Fc gamma RIIIA/CD16a protein

Human Fc gamma RIIIA (F176) (Gly17-Gln208) Accession # P08637-1	HHHHHHH	Avi-tag
N-terminus		C-terminus

Accession #

P08637-1

N-terminal Sequence
Analysis

Gly17

Structure / Form

Biotinylated protein via Avi-tag

Predicted Molecular Mass

25 kDa

SDS-PAGE

48-57 kDa, under reducing conditions

Product Datasheets

AVI8894

Product Datasheet

COA

SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

AVI8894

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution	Reconstitute at 200 μg/mL in PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Scientific Data

Binding Activity

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When Human IgG is immobilized at 5 µg/mL, Biotinylated Recombinant Human Fc gamma RIIIA/CD16a (F176) His-tag Avi-tag (Catalog # AVI8894) binds with an ED₅₀ of 50-300 ng/mL.

SDS-PAGE

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2 μg/lane of Biotinylated Recombinant Human Fc gamma RIIIA/CD16a (F176) His-tag Avi-tag (Catalog # AVI8894) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 48-57 kDa.

Surface Plasmon Resonance (SPR)

Surface plasmon resonance sensorgram of Avi-tag biotinylated human Fc gamma RIIIA protein binding to normal human IgG

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Avi-tag Biotinylated Recombinant Human Fc gamma RIIIA His protein (Catalog # AVI8894) was immobilized on a Biacore Sensor Chip CM5 via the Avi-tag biotin, and binding to Normal Human IgG was measured at a concentration range between 10.4 nM and 6.67 µM. The double-referenced sensorgram was fit to a 1:1 binding model to determine the binding kinetics and affinity, with an affinity constant of KD=0.379 µM.

Reconstitution Calculator

Background: Fc gamma RIIIA/CD16a

Fc gamma RIIIa is a low/intermediate affinity receptor for polyvalent immune-complexed IgG. It is involved in phagocytosis, secretion of enzymes and inflammatory mediators, antibody-dependent cytotoxicity and clearance of immune complexes (1, 2). In humans, it is a 50-70 kDa type I transmembrane activating receptor expressed by NK cells, T cells, monocytes, and macrophages (1). Fc gamma RIIIb is highly related, sharing 97% amino acid (aa) identity within the extracellular domain (ECD), but is a GPI-linked receptor expressed on human neutrophils and eosinophils (1, 2). The ECD of Fc gamma RIIIa shares 63%, 61%, 65%, 59% and 58% aa identity with mouse Fc gamma RIV, rat Fc gamma RIIIa, feline CD16, bovine CD16 and porcine Fc gamma RIIIb paralogs, respectively. The Fc gamma RIIIa cDNA encodes 254 aa including a 16 aa signal sequence, 192 aa ECD with two C2-type Ig-like domains and five potential N-glycosylation sites, a 21 aa transmembrane (TM) sequence and a 25 aa cytoplasmic domain. In humans, a single nucleotide polymorphism creates high binding (V176) and low binding (F176) forms that, when homozygous, may influence susceptibility to autoimmune diseases or response to therapeutic IgG antibodies (3, 4). Catalog # AVI8894 is expressed as the F176 isoform of Fc gamma RIIIa. Fc gamma RIIIa surface expression requires interaction of an accessory chain, either the common gamma -chain or CD3 zeta (5, 6). Glycosylation patterns, electrophoretic mobility and binding affinity appear to differ between NK cell and monocyte Fc gamma RIIIa (7). The ECD of both Fc gamma RIIIa and b can be proteolytically cleaved and retain binding activity in soluble form (8-11). In monocytes and macrophages, activation and phagocytosis can trigger Fc gamma RIIIa release (11). Soluble Fc gamma RIII can be detected in normal plasma and is increased in rheumatoid arthritis and in coronary artery diseases (9, 10).

References

Nimmerjahn, F. and J.V. Ravetch (2006) Immunity 24:19.
Ravetch, J.V. and B. Perussia (1989) J. Exp. Med. 170:481.
Wu, J. et al. (1997) J. Clin. Invest. 100:1059.
Dall'Ozzo, S. et al. (2004) Cancer Res. 64:4664.
Kim, M.-K. et al. (2003) Blood 101:4479.
Lanier, L.L. et al. (1989) Nature 342:803.
Edberg, J.C. and R.P. Kimberley (1997) J. Immunol. 159:3849.
Li, P. et al. (2007) J. Biol. Chem. 282:6210.
Masuda, M. et al. (2003) J. Rheumatol. 30:1911.
Masuda, M. et al. (2006) Atherosclerosis 188:377.
Webster, N.L. et al.(2006) J. Leukoc. Biol. 79:294.

Long Name

Fc gamma Receptor III A

Entrez Gene IDs

2214 (Human); 304966 (Rat)

Alternate Names

CD16a antigen; CD16a; CD16FCRIIIA; Fc fragment of IgG, low affinity IIIa, receptor (CD16a); Fc fragment of IgG, low affinity IIIa, receptor for (CD16); Fc gamma receptor III-A; Fc gamma RIIIA; FCG3; Fc-gamma receptor III-2 (CD 16); Fc-gamma receptor IIIb (CD16); Fc-gamma RIII; Fc-gamma RIIIa; Fc-gamma RIII-alpha; FCGR3; FCGR3A; FCGRIII; FcgRIIIA; FcR-10; FCRIII; FcRIIIa; IGFR3FcgammaRIIIA; immunoglobulin G Fc receptor III; low affinity III, receptor for (CD16); low affinity immunoglobulin gamma Fc region receptor III-A; neutrophil-specific antigen NA