Recombinant Human FOLR1 Protein, CF Summary
Product Specifications
Arg25-Met233, with a C-terminal 6x-His tag
Analysis
Product Datasheets
Carrier Free
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
5646-FR
| Formulation | Lyophilized from a 0.2 μm filtered solution in PBS. |
| Reconstitution | Reconstitute at 100 μg/mL in PBS. |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage: | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
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Reconstitution Calculator
Background: FOLR1
Folate Receptor 1 (FOLR1), also known as Folate Receptor alpha and Folate Binding Protein (FBP), is a 37 - 42 kDa protein that mediates the cellular uptake of folic acid and reduced folates. Dietary folates are required for many key metabolic processes including nucleotide and methionine synthesis, the interconversion of glycine and serine, and histidine breakdown (1, 2). Mature FOLR1 is an N-glycosylated protein that is anchored to the cell surface by a GPI linkage (3 - 6). Human FOLR1 shares 83% amino acid sequence identity with mouse and rat FOLR1. FOLR1 is predominantly expressed on epithelial cells and is dramatically upregulated on many carcinomas (7, 8). It is critically required during early embryogenesis as shown in knockout mice which die in utero with gross morphological defects (9). FOLR1 is internalized to the endosomal system where it dissociates from its ligand before recycling to the cell surface (6, 10). A soluble form of FOLR1 can be proteolytically shed from the cell surface into the serum and breast milk (11).
- Kelemen, L.E. (2006) Int. J. Cancer 119:243.
- Fowler, B. et al. (2001) Kidney Int. 59:S-221.
- Luhrs, C.A. et al. (1989) J. Biol. Chem. 264:21446.
- Lacey, S.W. et al. (1989) J. Clin. Invest. 84:715.
- Elwood, P.C. (1989) J. Biol. Chem. 264:14893.
- Rijnboutt, S. et al. (1996) J. Cell Biol. 132:35.
- Ross, J.F. et al. (1994) Cancer 73:2432.
- Parker, N. et al. (2005) Anal. Biochem. 338:284.
- Piedrahita, J.A. et al. (1999) Nat. Genet. 23:228.
- Paulos, C.M. et al. (2004) Mol. Pharmacol. 66:1406.
- Elwood, P.C. et al. (1991) J. Biol. Chem. 26:2346.
Citations for Recombinant Human FOLR1 Protein, CF
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 6
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Safety and anti-tumour activity of the IgE antibody MOv18 in patients with advanced solid tumours expressing folate receptor-alpha: a phase I trial
Authors: Spicer, J;Basu, B;Montes, A;Banerji, U;Kristeleit, R;Miller, R;Veal, GJ;Corrigan, CJ;Till, SJ;Figini, M;Canevari, S;Barton, C;Jones, P;Mellor, S;Carroll, S;Selkirk, C;Nintos, G;Kwatra, V;Funingana, IG;Doherty, G;Gould, HJ;Pellizzari, G;Nakamura, M;Ilieva, KM;Khiabany, A;Stavraka, C;Chauhan, J;Gillett, C;Pinder, S;Bax, HJ;Josephs, DH;Karagiannis, SN;
Nature communications
Species: Human
Sample Types: Serum
Applications: ELISA Capture -
Folate receptor alpha in ovarian cancer tissue and patient serum is associated with disease burden and treatment outcomes
Authors: HJ Bax, J Chauhan, C Stavraka, A Santaolall, G Osborn, A Khiabany, M Grandits, J López-Aben, LCGF Palhares, C Chan Wah H, A Robinson, A Pope, N Woodman, C Naceur-Lom, S Malas, JEM Coumbe, M Nakamura, R Laddach, S Mele, S Crescioli, AM Black, S Lombardi, S Canevari, M Figini, A Sayasneh, S Tsoka, K FitzGerald, C Gillett, S Pinder, M Van Hemelr, R Kristeleit, S Ghosh, A Montes, J Spicer, SN Karagianni, DH Josephs
British Journal of Cancer, 2022-11-19;0(0):.
Species: Human
Sample Types: Serum, Whole Cells
Applications: Bioassay, ELISA Capture -
Validation of Serum Biomarkers That Complement CA19-9 in Detecting Early Pancreatic Cancer Using Electrochemiluminescent-Based Multiplex Immunoassays
Authors: J Song, LJ Sokoll, DW Chan, Z Zhang
Biomedicines, 2021-12-14;9(12):.
Species: Human
Sample Types: Protein
Applications: Reference Standard -
Evaluation of Antigen-Conjugated Fluorescent Beads to Identify Antigen-Specific B Cells
Authors: I Correa, KM Ilieva, S Crescioli, S Lombardi, M Figini, A Cheung, JF Spicer, ANJ Tutt, FO Nestle, P Karagianni, KE Lacy, SN Karagianni
Front Immunol, 2018-03-23;9(0):493.
Species: Human
Sample Types: Recombinant Protein
Applications: Bioassay -
Conditions associated with circulating tumor-associated folate receptor 1 protein in healthy men and women.
Authors: Kelemen L, Brenton J, Parkinson C, C Whitaker H, Piskorz A, Csizmadi I, Robson P
PLoS ONE, 2014-05-08;9(5):e96542.
Applications: ELISA (Standard) -
Harnessing engineered antibodies of the IgE class to combat malignancy: initial assessment of FcvarepsilonRI-mediated basophil activation by a tumour-specific IgE antibody to evaluate the risk of type I hypersensitivity.
Authors: Rudman SM, Josephs DH, Cambrook H, Karagiannis P, Gilbert AE, Dodev T, Hunt J, Koers A, Montes A, Taams L, Canevari S, Figini M, Blower PJ, Beavil AJ, Nicodemus CF, Corrigan C, Kaye SB, Nestle FO, Gould HJ, Spicer JF, Karagiannis SN
Clin. Exp. Allergy, 2011-05-16;41(10):1400-1413.
Species: Human
Sample Types: N/A, Serum, Whole Cells
Applications: ELISA (Capture), ELISA (Standard), Flow Cytometry
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