Recombinant Human FOLR2 Protein, CF

Catalog #: 5697-FR Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
5697-FR-050

R&D Systems Recombinant Proteins and Enzymes

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Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Human FOLR2 Protein, CF Summary

Product Specifications

Purity

>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Endotoxin Level

<0.01 EU per 1 μg of the protein by the LAL method.

Activity

Measured by its binding ability in a functional ELISA. When Folic Acid-Bovine Serum Albumin was coated at 5 μg/mL (100 μL/well), the concentration of Recombinant Human FOLR-2 that produces 50% of the optimal binding response was found to be <1 nM.

Source

Chinese Hamster Ovary cell line, CHO-derived human FOLR2 protein
Gln22-His228, with a C-terminal 6-His tag

Accession #

P14207

N-terminal Sequence
Analysis

No results obtained: Gln22 predicted

Predicted Molecular Mass

25.4 kDa

SDS-PAGE

25-40 kDa, reducing conditions

Product Datasheets

5697-FR

Product Datasheet

COA

SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

5697-FR

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution	Reconstitute at 100 μg/mL in PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Reconstitution Calculator

Background: FOLR2

Folate Receptor 2 (FOLR2), also known as Folate Receptor beta, is a 38 kDa protein that mediates the cellular uptake of folic acid and reduced folates. Dietary folates are required for many key metabolic processes including nucleotide and methionine synthesis, the interconversion of glycine and serine, and histidine breakdown (1, 2). Mature FOLR2 is an N-glycosylated protein that is anchored to the cell surface by a GPI linkage (3, 4). Human FOLR2 shares 83% amino acid sequence identity with mouse and rat FOLR2. FOLR2 is predominantly expressed in placenta, cells of the neutrophilic lineage, and some CD34+ hematopoietic progenitor cells (4 - 6). It is upregulated on myeloid leukemias, head and neck squamous cell carcinomas, and several nonepithelial cancers (3, 5, 7). It is also upregulated on macrophages and monocytes at chronic inflammatory sites including rheumatoid arthritis synovium and glioblastoma (8 - 10). FOLR2 knockout mice do not show gross morphological defects, but they exhibit increased sensitivity to arsenate-induced teratogenicity (11, 12).

References

Fowler, B. et al. (2001) Kidney Int. 59:S-221.
Kelemen, L.E. (2006) Int. J. Cancer 119:243.
Shen, F. et al. (1994) Biochemistry 33:1209.
Ratnam, M. et al. (1989) Biochemistry 28:8249.
Ross, J.F. et al. (1999) Cancer 85:348.
Reddy, J.A. et al. (1999) Blood 93:3940.
Ross, J.F. et al. (1994) Cancer 73:2432.
Nakashima-Matsushita, N. et al. (1999) Arthritis Rheum. 42:1609.
van der Heijden, J.W. et al. (2009) Arthritis Rheum. 60:12.
Nagai, T. et al. (2009) Cancer Immunol. Immunother. Feb 24 epub.
Piedrahita, J.A. et al. (1999) Nat. Genet. 23:228.
Wlodarczyk, B. et al. (2001) Toxicol. Appl. Pharmacol. 177:238.

Long Name

Folate Receptor 2

Entrez Gene IDs

2350 (Human); 14276 (Mouse); 293154 (Rat)

Alternate Names

beta-HFR; FBP; FBP/PL-1; folate receptor 2 (fetal); Folate receptor 2; folate receptor beta; folate receptor, beta; Folate receptor, fetal/placental; folate-binding protein, fetal/placental; FOLR2; FR-beta; FR-P3; Placental folate-binding protein