Recombinant Human HVEM/TNFRSF14 Fc Chimera Protein

Name: Recombinant Human HVEM/TNFRSF14 Fc Chimera Protein
Brand: R&D Systems
SKU: 356-HV

Catalog #: 356-HV
3 Citations Datasheet / COA / SDS

Newer Version Available: 11177-HV

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Discontinued Product

356-HV has been discontinued and is replaced by 11177-HV.

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Product Details

Citations (3)

FAQs

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Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Human HVEM/TNFRSF14 Fc Chimera Protein Summary

Product Specifications

Purity

>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<1.0 EU per 1 μg of the protein by the LAL method.

Activity

Measured by its ability to inhibit TNF-beta -mediated cytotoxicity using L‑929 mouse fibroblast cells. The ED₅₀ for this effect is 2.5-10 µg/mL in the presence of 50 pg/mL of Recombinant Human TNF‑ beta /TNFSF1 (Catalog # 211-TB).

Source

Mouse myeloma cell line, NS0-derived human HVEM/TNFRSF14 protein

Human HVEM Pro37-Val202 (Ser108Thr; Ala140Arg) Accession # Q92956.3	IEGRMD	Human IgG₁ (Pro100-Lys330)	6-His tag
N-terminus			C-terminus

Accession #

Q92956.3

N-terminal Sequence
Analysis

Pro37

Structure / Form

Disulfide-linked homodimer

Predicted Molecular Mass

45 kDa (monomer)

SDS-PAGE

60 kDa, reducing conditions

Product Datasheets

356-HV (with carrier)

Product Datasheet

COA

SDS

356-HV/CF (carrier free)

Product Datasheet

COA

SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

356-HV

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Reconstitution	Reconstitute at 500 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

356-HV/CF

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution	Reconstitute at 500 μg/mL in sterile PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Reconstitution Calculator

Background: HVEM/TNFRSF14

HVEM (herpesvirus entry mediator), also known as TNFRSF14 and CD270, is a type I membrane protein in the TNF receptor superfamily, and it can both promote and inhibit T cell activity (1). Mature human HVEM consists of a 164 amino acid (aa) extracellular domain (ECD) with three cysteine-rich domains (CRD), a 21 aa transmembrane segment, and a 60 aa cytoplasmic tail with a TRAF interaction domain (2, 3). Within the ECD, human HVEM shares 55% aa sequence identity with mouse and rat HVEM. Alternative splicing generates an additional isoform with a substitution of the N-terminal 10 amino acids including the signal peptide. HVEM is highly expressed on naïve CD4⁺ T cells, CD8⁺ T memory cells, regulatory T cells, dendritic cells, monocytes, and neutrophils (4-8). Its expression declines during effector T cell activation but is up-regulated during Treg activation (4, 5). HVEM functions as a receptor for BTLA, CD160, LIGHT/TNFSF14, and Lymphotoxin-alpha (4, 9‑12). Ligation of HVEM by LIGHT triggers T cell, monocyte, and neutrophil activation (8, 10) and contributes to Th1 inflammation and cardiac allograft rejection (13, 14). In contrast, HVEM binding to CD160 or BTLA suppresses T cell and dendritic cell activation (4, 7, 9, 10) and dampens intestinal inflammation (15). HVEM enhances the development of CD8⁺ T cell memory and Treg function (5, 6). It is additionally expressed on intestinal epithelial cells, where its binding by intraepithelial lymphocyte (IEL) expressed CD160 promotes epitheilal integrity and host defense (16). The herpesvirus envelope glycoprotein gD, which binds HVEM to initiate membrane fusion, can antagonize both BTLA and LIGHT binding (2, 9, 11).

References

del Rio, M.L. et al. (2010) J. Leukoc. Biol. 87:223.
Montgomery, R.I. et al. (1996) Cell 87:427.
Hsu, H. et al. (1997) J. Biol. Chem. 272:13471.
Sedy, J. R. et al. (2005) Nat. Immunol. 6:90.
Tao, R. et al. (2008) J. Immunol. 180:6649.
Steinberg, M.W. et al. (2013) PLoS One 8:e77992.
de Trez, C. et al. (2008) J. Immunol. 180:238.
Heo, S.K. et al. (2006) J. Leukoc. Biol. 79:330.
Gonzalez, L.C. et al. (2005) Proc. Natl. Acad Sci. USA 102:1116.
Cai, G. et al. (2008) Nat. Immunol. 9:176.
Mauri, D.N. et al. (1998) Immunity 8:21.
Harrop, J.A. et al. (1998) J. Biol. Chem. 273:27548.
Wang, J. et al. (2005) J. Immunol. 174:8173.
Ye, Q. et al. (2002) J. Exp. Med. 195:795.
Steinberg, M.W. et al. (2008) J. Exp. Med. 205:1463.
Shui, J.W. et al. (2012) Nature 488:222.

Long Name

Herpesvirus Entry Mediator

Entrez Gene IDs

8764 (Human); 230979 (Mouse); 102137807 (Cynomolgus Monkey)

Alternate Names

ATAR; CD270 antigen; CD270; CD40-like protein; Herpes virus entry mediator A; Herpesvirus entry mediator A; HveA; HVEM; HVEMTR2HVEAATAR; LIGHTR; TNFRSF14; tumor necrosis factor receptor superfamily member 14; tumor necrosis factor receptor superfamily, member 14 (herpesvirus entrymediator); Tumor necrosis factor receptor-like 2; tumor necrosis factor receptor-like gene2

Related Research Areas

Citations for Recombinant Human HVEM/TNFRSF14 Fc Chimera Protein

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

3 Citations: Showing 1 - 3
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Loss of the HVEM Tumor Suppressor in Lymphoma and Restoration by Modified CAR-T Cells.
Authors: Boice M, Salloum D, Mourcin F, Sanghvi V, Amin R, Oricchio E, Jiang M, Mottok A, Denis-Lagache N, Ciriello G, Tam W, Teruya-Feldstein J, de Stanchina E, Chan W, Malek S, Ennishi D, Brentjens R, Gascoyne R, Cogne M, Tarte K, Wendel H
Cell, 2016-09-29;167(2):405-418.e13.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay
CD160 isoforms and regulation of CD4 and CD8 T-cell responses.
Authors: El-Far, Mohamed, Pellerin, Charles, Pilote, Louise, Fortin, Jean-Fra, Lessard, Ivan A D, Peretz, Yoav, Wardrop, Elizabet, Salois, Patrick, Bethell, Richard, Cordingley, Michael, Kukolj, George
J Transl Med, 2014-09-02;12(0):217.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay
Creation of a LIGHT mutant with the capacity to evade the decoy receptor for cancer therapy.
Authors: Morishige T, Yoshioka Y, Inakura H, Tanabe A, Yao X, Tsunoda S, Tsutsumi Y, Mukai Y, Okada N, Nakagawa S
Biomaterials, 2010-02-01;31(12):3357-63.
Applications: Surface Plasmon Resonance