Does IL-1 beta induce NO in smooth muscle?

Name: Recombinant Human IL-1 beta/IL-1F2, Animal-Free Protein
Brand: R&D Systems
SKU: AFL201
Price: 6289 USD

Yes it does, based on the literature.

Recombinant Human IL-1 beta/IL-1F2, Animal-Free Protein

Catalog #: AFL201
1 Citation Datasheet / COA / SDS

GMP Version Available: 201-GMP

GMP

Intended for preclinical researchers who may transition to GMP IL-1beta for their clinical work

Catalog #	Availability	Size / Price	Qty
AFL201
AFL201-025

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Product Details

Citations (1)

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Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Human IL-1 beta/IL-1F2, Animal-Free Protein Summary

Learn more about Animal-Free Recombinant Proteins

Animal Free Proteins
Animal-free proteins are particularly important for researchers concerned with experimental variables caused by trace animal components or mammalian pathogens. Our products generated under animal-free conditions share the same biological activities as those produced using our standard laboratory techniques.

Product Specifications

Purity

>97%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.01 EU per 1 μg of the protein by the LAL method.

Activity

Measured in a cell proliferation assay using D10.G4.1 mouse helper T cells. Symons, J.A. et al. (1987) in Lymphokines and Interferons, a Practical Approach. Clemens, M.J. et al. (eds): IRL Press. 272. The ED₅₀ for this effect is <12 pg/mL.

The specific activity of Recombinant Human IL-1 beta is >5.0 x 10⁷ IU/mg, which is calibrated against recombinant human IL-1 beta WHO International Standard (NIBSC code: 86/680).

Source

E. coli-derived human IL-1 beta/IL-1F2 protein
Ala117-Ser269
Produced using non-animal reagents in an animal-free laboratory.

Accession #

NP_000567.1

N-terminal Sequence
Analysis

Ala117 & Pro118

Predicted Molecular Mass

17 kDa

Product Datasheets

AFL201

Product Datasheet

COA

SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

AFL201

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution	Reconstitute at 0.2 mg/mL in sterile PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 12 months, 2 to 8 °C under sterile conditions after reconstitution.

Reconstitution Calculator

Background: IL-1 beta/IL-1F2

IL-1 is a name that designates two pleiotropic cytokines, IL-1 alpha (IL-1F1) and IL-1 beta (IL-1F2), which are the products of distinct genes. IL-1 alpha and IL-1 beta are structurally related polypeptides that share approximately 21% amino acid (aa) identity in human. Both proteins are produced by a wide variety of cells in response to inflammatory agents, infections, or microbial endotoxins. While IL-1 alpha and IL-1 beta are regulated independently, they bind to the same receptor and exert identical biological effects. IL-1 RI binds directly to IL-1 alpha or IL-1 beta and then associates with IL-1 R accessory protein (IL‑1 R3/IL‑1 R AcP) to form a high-affinity receptor complex that is competent for signal transduction. IL-1 RII has high affinity for IL-1 beta but functions as a decoy receptor and negative regulator of IL-1 beta activity. IL-1ra functions as a competitive antagonist by preventing IL-1 alpha and IL-1 beta from interacting with IL-1 RI (1‑4). The human IL-1 beta cDNA encodes a 269 aa precursor. A 116 aa propeptide is cleaved intracellularly by the cysteine protease IL-1 beta -converting enzyme (Caspase-1/ICE) to generate the active cytokine (5‑7). The 17 kDa mature human IL-1 beta shares 96% aa sequence identity with rhesus and 67%‑78% with canine, cotton rat, equine, feline, mouse, porcine, and rat IL‑1 beta.

References

Allan, S.M. et al. (2005) Nat. Rev. Immunol. 5:629.
Boraschi, D. and A. Tagliabue (2006) Vitam. Horm. 74:229.
Kornman, K.S. (2006) Am. J. Clin. Nutr. 83:475S.
Isoda, K. and F. Ohsuzu (2006) J. Atheroscler. Thromb. 13:21.
March, C.J. et al. (1985) Nature 315:641.
Auron, P.E. et al. (1984) Proc. Natl. Acad. Sci. 81:7907.
Martinon, F. and J. Tschopp (2007) Cell Death Differ. 14:10.

Long Name

Interleukin 1 beta

Entrez Gene IDs

3553 (Human); 16176 (Mouse); 24494 (Rat); 397122 (Porcine); 403974 (Canine); 100034237 (Equine); 100135556 (Guinea Pig)

Alternate Names

catabolin; IL1 beta; IL-1 beta; IL-1; IL1B; IL-1b; IL1-BETA; IL-1F2; IL1F2IL-1 beta; interleukin 1, beta; interleukin-1 beta; preinterleukin 1 beta; pro-interleukin-1-beta

Manufacturing Specifications

Animal-Free Manufacturing Conditions
Our dedicated controlled-access animal-free laboratories ensure that at no point in production are the products exposed to potential contamination by animal components or byproducts. Every stage of manufacturing is conducted in compliance with R&D Systems' stringent Standard Operating Procedures (SOPs). Production and purification procedures use equipment and media that are confirmed animal-free.

Production

All molecular biology procedures use animal-free media and dedicated labware.
Dedicated fermentors are utilized in committed animal-free areas.

Purification

Protein purification columns are animal-free.
Bulk proteins are filtered using animal-free filters.
Purified proteins are stored in animal-free containers in a dedicated cold storage room.

Quality Assurance

Low Endotoxin Level.
No impairment of biological activity.
High quality product obtained under stringent conditions.
For ex vivo research or bioproduction, additional documentation can be provided.

Please read our complete Animal-Free Statement

Product Specific Notices

This product is covered by one or more of the following U.S. patents: 5,681,933 and 5,474,899.

Related Research Areas

Citation for Recombinant Human IL-1 beta/IL-1F2, Animal-Free Protein

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

IL-15 promotes activation and expansion of CD8+ T cells in HIV-1 infection
Authors: Souheil-Antoine Younes
J Clin Invest, 2016-06-20;126(7):2745-2756.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay