Recombinant Human IL-3R alpha/CD123 Protein

Catalog #: 301-R3
2 Citations Datasheet / COA / SDS

Carrier Free

Catalog #	Availability	Size / Price	Qty
301-R3-025/CF

With Carrier

Catalog #	Availability	Size / Price	Qty
301-R3-025

Recombinant Human IL-3R alpha/CD123 Protein Binding Activity

2 Images

Product Details

Citations (2)

Reviews

Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Human IL-3R alpha/CD123 Protein Summary

Product Specifications

Purity

>97%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

Activity

Measured by its binding ability in a functional ELISA. When Recombinant Human IL‑3 R alpha /CD123 is present at 1 μg/mL, the concentration of Recombinant Human IL‑3 (Catalog # 203-IL) that produces 50% of the optimal binding response is found to be approximately 8‑40 ng/mL.

Source

Mouse myeloma cell line, NS0-derived human IL-3 R alpha/CD123 protein
Lys20-Arg305

Accession #

P26951

N-terminal Sequence
Analysis

Lys20

Predicted Molecular Mass

33 kDa

Product Datasheets

301-R3 (with carrier)

Product Datasheet

COA

SDS

301-R3/CF (carrier free)

Product Datasheet

COA

SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

301-R3

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Reconstitution	Reconstitute at 50 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

301-R3/CF

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution	Reconstitute at 100 μg/mL in sterile PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Scientific Data

Binding Activity

View Larger

Recombinant Human IL-3 Ra (Catalog # 301-R3) binds Recombinant Human IL-3 in a functional ELISA. When Recombinant Human IL-3 Ra is present at 1 µg/mL, the concentration of Recombinant Human IL-3 (Catalog # 203-IL) that produces 50% of the optimal binding response is approximately 8-40 ng/mL.

SDS-PAGE

View Larger

1 μg/lane of Recombinant Human IL-3 R alpha / CD123 was resolved with SDS-PAGE under reducing (R) conditions and visualized by silver staining, showing bands at 55 kDa and 58 kDa.

Reconstitution Calculator

Background: IL-3R alpha

Interleukin 3 is a pleiotropic cytokine produced primarily by activated T cells or mast cells. IL‑3 stimulates the proliferation and differentiation of hemopoietic cells including the pluripotent hematopoietic stem cells as well as various lineage‑committed cells. The biological effects of IL‑3 on the various cell types are mediated by the binding of IL‑3 to specific cell surface receptor complexes. The functional high‑affinity human IL‑3 receptor is a heterodimer consisting of a ligand binding alpha subunit and the beta subunit. The alpha subunit alone binds IL‑3 with low affinity. The beta subunit does not bind IL‑3 by itself but is required for the high‑affinity binding of IL‑3 to the heterodimeric receptor complex. The beta subunit has also been found to be a component of the high‑affinity receptor complex for IL‑5 and GM‑CSF. Both the alpha and the beta subunits are members of the cytokine receptor superfamily.

References

Ogorochi, T. and A. Miyajima (1994) in Guidebook to Cytokines and Their Receptors, N.A. Nicola, ed. Oxford University, New York p. 40.

Long Name

Interleukin 3 Receptor alpha

Entrez Gene IDs

3563 (Human); 16188 (Mouse); 102138639 (Cynomolgus Monkey)

Alternate Names

CD123 antigen; CD123; hIL3Ra; hIL-3Ra; IL-3 R alpha; IL-3 receptor subunit alpha; IL3R alpha; IL-3R alpha; IL-3R subunit alpha; IL3R; IL3RA; IL-3Ra; IL-3R-alpha; IL3RAY; IL3RX; IL3RY; interleukin 3 receptor, alpha (low affinity); interleukin-3 receptor subunit alpha; MGC34174

Related Research Areas

Citations for Recombinant Human IL-3R alpha/CD123 Protein

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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CD123-Engager T Cells as a Novel Immunotherapeutic for Acute Myeloid Leukemia
Mol Ther, 2016-06-06;0(0):.
Species: Human
Sample Types: Cell Culture Supernates
Applications: CAR-T (ELISA capture)
CD123 targeting oncolytic adenoviruses suppress acute myeloid leukemia cell proliferation in vitro and in vivo.
Authors: Li, G, Li, X, Wu, H, Yang, X, Zhang, Y, Chen, L, Wu, X, Cui, L, Wu, L, Luo, J, Liu, X Y
Blood Cancer J, 2014-03-21;4(0):e194.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay