Recombinant Human IL-5 R alpha/CD125 Avi-tag His Protein, CF

Catalog #: AVI11394 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AVI11394-025

Biotinylated Recombinant Human IL‑5 R alpha /CD125 Avi-tag His-tag Protein Binding Activity.

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All IL-5 R alpha/CD125 Proteins and Enzymes

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Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Human IL-5 R alpha/CD125 Avi-tag His Protein, CF Summary

Product Specifications

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

Activity

Measured by its binding ability in a functional ELISA. Biotinylated Recombinant Human IL‑5 R alpha /CD125 Avi-tag His-tag (Catalog # AVI11394) binds to Recombinant Human IL-5 Protein (Catalog # 205-IL) with a ED₅₀ of 0.030-0.300 μg/mL.

Source

Chinese Hamster Ovary cell line, CHO-derived human IL-5 R alpha/CD125 protein

Human IL-5 R alpha (Asp21-Glu335) Accession # Q01344.2	Avi-tag	6-His tag
N-terminus		C-terminus

Accession #

Q01344.2

N-terminal Sequence
Analysis

Asp21

Structure / Form

Biotinylated via Avi-tag

Predicted Molecular Mass

40 kDa

SDS-PAGE

52-63 kDa, under reducing conditions.

Product Datasheets

AVI11394

Product Datasheet

COA

SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

AVI11394

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution	Reconstitute at 250 μg/mL in PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Scientific Data

Binding Activity

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Measured by its binding ability in a functional ELISA. Biotinylated Recombinant Human IL-5 R alpha /CD125 Avi-tag His-tag Protein (Catalog # AVI11394) binds to Recombinant Human IL-5 Protein (205-IL) with a ED₅₀ of 0.030 -0.300 μg/mL.

SDS-PAGE

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2 μg/lane of Biotinylated Recombinant Human IL‑5 R alpha /CD125 Avi-tag His-tag Protein (Catalog # AVI11394) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 52-63 kDa, under reducing conditions.

Reconstitution Calculator

Background: IL-5 R alpha/CD125

Interleukin‑5 Receptor alpha (IL‑5 R alpha ), also known as CD125, is a 60 kDa hematopoietin receptor that plays a dominant role in eosinophil biology (1‑3). Mature human IL‑5 R alpha consists of a 322 amino acid (aa) extracellular domain (ECD) with a WSxWS motif and a four cysteine motif, a 20 aa transmembrane segment, and a 58 aa cytoplasmic domain (4, 5). Within the ECD, human IL-5 R alpha shares 71% aa sequence identity with mouse and rat IL‑5 R alpha. Alternate splicing of human IL‑5 R alpha generates soluble secreted forms which function as IL‑5 antagonists (5‑7). The high affinity receptor for IL‑5 is a complex that consists of the ligand binding IL‑5 R alpha and the transmembrane common beta chain ( beta c/CD131) which is shared with the receptor complexes for IL‑3 and GM‑CSF (4). IL‑5 R alpha binds IL‑5 at low affinity and then associates with preformed beta c oligomers to form the signaling‑competent receptor complex (8). IL‑5 stimulation of CD34+ hematopoietic progenitor cells induces the up‑regulation of transmembrane IL‑5 R alpha followed by eosinophilic differentiation and activation (9‑11). IL‑5 R alpha also promotes the differentiation of basophils and B cells (12, 13). Exposure of mature eosinophils to IL‑5 attenuates their IL‑5 responsiveness by inducing the down‑regulation of surface IL‑5 R alpha and increased production of soluble IL‑5 R alpha (14, 15). Elevated production of IL‑5 at sites of allergic inflammation induces eosinophilia and exacerbation of immune cell infiltration, tissue damage, and remodeling (2, 3). Our Avi-tag Biotinylated human IL-5 R alpha Fc chimera features biotinylation at a single site contained within the Avi-tag, a unique 15 amino acid peptide. Protein orientation will be uniform when bound to streptavidin-coated surface due to the precise control of biotinylation and the rest of the protein is unchanged so there is no interference in the protein's bioactivity.

References

Martinez-Moczygemba, M. and D.P. Huston (2003) J. Allergy Clin. Immunol. 112:653.
Rothenberg, M.E. and S.P. Hogan (2005) Annu. Rev. Immunol. 24:147.
Elsas, X.P. and M.I.G. Elsas (2007) Curr. Med. Chem. 14:1925.
Tavernier, J. et al. (1991) Cell 66:1175.
Murata, Y. et al. (1992) J. Exp. Med. 175:341.
Tavernier, J. et al. (1992) Proc. Natl. Acad. Sci. 89:7041.
Cameron, L. et al. (2000) J. Immunol. 164:1538.
Zaks-Zilberman, M. et al. (2008) J. Biol. Chem. 283:13398.
Tavernier, J. et al. (2000) Blood 95:1600.
Clutterbuck, E.J. et al. (1989) Blood 73:1504.
Lopez, A.F. et al. (1988) J. Exp. Med. 167:219.
Denburg, J.A. et al. (1991) Blood 77:1462.
Hasbold, J. et al. (2004) Nat. Immunol. 5:55.
Gregory, B. et al. (2003) J. Immunol. 170:5359.
Liu, L.Y. et al. (2002) J. Immunol. 169:6459.

Long Name

Interleukin 5 Receptor alpha

Entrez Gene IDs

3568 (Human); 16192 (Mouse)

Alternate Names

CD125 antigen; CD125; CDw125CD125; HSIL5R3; IL-5 R alpha; IL-5 receptor subunit alpha; IL5R alpha; IL-5R subunit alpha; IL5R; IL5RA; IL-5Ra; IL-5R-alpha; interleukin 5 receptor type 3; interleukin 5 receptor, alpha; interleukin-5 receptor alpha chain; interleukin-5 receptor alpha subunit; interleukin-5 receptor subunit alpha; MGC26560