Recombinant Human Ly6K Fc Chimera Protein, CF

Catalog #: 10648-L6 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
10648-L6-050

Recombinant Human Ly6K Fc Chimera Protein Bioactivity

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Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Human Ly6K Fc Chimera Protein, CF Summary

Product Specifications

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

Activity

Measured by its ability to inhibit anti-CD3 antibody induced IL-2 or IFN-gamma secretion by human T cells. The ED₅₀ for this effect is 0.75-7.5 μg/mL.

Source

Chinese Hamster Ovary cell line, CHO-derived human Ly6K protein

Human Ly6K (Asp18-Gly138) Accession # Q17RY6.2	IEGRMD	Human IgG₁ (Pro100-Lys330)
N-terminus		C-terminus

Accession #

Q17RY6.2

N-terminal Sequence
Analysis

Asp18

Structure / Form

Disulfide-linked homodimer

Predicted Molecular Mass

41 kDa

SDS-PAGE

41-61 kDa, under reducing conditions

Product Datasheets

10648-L6

Product Datasheet

COA

SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

10648-L6

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution	Reconstitute at 200 μg/mL in PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Scientific Data

Bioactivity

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Measured by its ability to inhibit anti-CD3 antibody induced IL-2 secretion by human T cells. The ED₅₀ for this effect is 0.75-7.5 μg/mL.

SDS-PAGE

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2 μg/lane of Recombinant Human Ly6K Fc Chimera Protein (Catalog # 10648-L6) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 41-61 kDa and 82-122 kDa, respectively.

Reconstitution Calculator

Background: Ly6K

Lymphocyte antigen 6 complex, locus K (Ly6K) is a member of the LY6/urokinase-type plasminogen activator receptors (uPARS) family which participate in a wide range of functions from cell proliferation and migration to cytokine production (1). In humans, there are over 30 Ly6/uPARS members, characterized by a conserved, three-fingered disulfide linked LU domain that creates structural motif and a glycosylphosphatidylinositol (GPI)-anchoring site (2, 3). Members of the family are categorized as transmembrane or secretory depending on the presence of a GPI-anchored signal sequence. Mature LY6K contains an extracellular domain (ECD) with a single LU domain and a GPI-anchoring site. Within the ECD, human Ly6K shares 39% and 36% amino acid sequence identity with mouse and rat Ly6K, respectively. Human Ly6K is strongly expressed in testis and is crucial for the production of fertile spermatozoa (4). Additionally, Ly6K has been shown to be upregulated in a number of types of cancer and promotes tumor cell proliferation and metastasis and is a potential cancer therapy target (5). Furthermore, Ly6K promotes glioblastoma tumorigenicity via caveolin-1–mediated ERK1/2 signaling (6). The over-expression of Ly6K suppresses T cell development in the in vivo mouse model (7).

References