Recombinant Human Mer Fc Chimera Protein, CF

Name: Recombinant Human Mer Fc Chimera Protein, CF
Brand: R&D Systems
SKU: 891-MR
Rating: 4.5 (2 reviews)

Catalog #: 891-MR
5 Citations 2 Reviews Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
891-MR-100

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Citations (5)

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Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Human Mer Fc Chimera Protein, CF Summary

Product Specifications

Purity

>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

Activity

Measured by its binding ability in a functional ELISA. Immobilized Recombinant Human (rh) Mer Fc Chimera at 10 µg/mL (100 µL/well) can bind rhGas6 (Catalog # 885-GSB) with a linear range of 0.03-1 µg/mL.

Source

Spodoptera frugiperda, Sf 21 (baculovirus)-derived human Mer protein

Human Mer (Arg26-Ala499) Accession # AAB60430.1	IEGRMD	Human IgG₁ (Pro100-Lys330)
N-terminus		C-terminus

Accession #

AAB60430.1

N-terminal Sequence
Analysis

Arg26

Structure / Form

Disulfide-linked homodimer

Predicted Molecular Mass

78 kDa (monomer)

SDS-PAGE

100-110 kDa, reducing conditions

Product Datasheets

891-MR

Product Datasheet

COA

SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

891-MR

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution	Reconstitute at 100 μg/mL in sterile PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Reconstitution Calculator

Background: Mer

Axl (Ufo, Ark), Dtk (Sky, Tyro3, Rse, Brt) and Mer (human and mouse homologues of chicken c-Eyk) constitute a receptor tyrosine kinase subfamily. The extracellular domains of these proteins contain two Ig-like motifs and two fibronectin type III motifs. This characteristic topology is also found in neural cell adhesion molecules and in receptor tyrosine phosphatases. These receptors bind the vitamin K-dependent protein growth-arrest-specific gene 6 (Gas6) which is structurally related to the anticoagulation factor protein S. Binding of Gas6 induces receptor autophosphorylation and downstream signaling pathways that can lead to cell proliferation, migration or the prevention of apoptosis. Recent studies suggest that this family of tyrosine kinase receptors may be involved in hematopoiesis, embryonic development, tumorigenesis and regulation of testicular functions.

References

Nagata, K. et al. (1996) J. Biol. Chem. 22:30022.
Crosier, K.E. and P.S Crosier (1997) Pathology 29:131.

Long Name

Receptor Tyrosine Protein Kinase Mer

Entrez Gene IDs

10461 (Human); 17289 (Mouse); 102143798 (Cynomolgus Monkey)

Alternate Names

c-Eyk; c-mer proto-oncogene tyrosine kinase; C-mer; EC 2.7.10; EC 2.7.10.1; MER receptor tyrosine kinase; Mer; MerTK; MGC133349; Receptor tyrosine kinase MerTK; RP38Proto-oncogene c-Mer; STK kinase; tyrosine-protein kinase Mer

Related Research Areas

Citations for Recombinant Human Mer Fc Chimera Protein, CF

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

5 Citations: Showing 1 - 5
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Ligand-dependent kinase activity of MERTK drives efferocytosis in human iPSC-derived macrophages
Authors: F Wanke, S Gutbier, A Rümmelin, M Steinberg, LD Hughes, M Koenen, J Komuczki, D Regan-Komi, S Wagage, J Hesselmann, R Thoma, D Brugger, T Christopei, H Wang, F Point, R Hallet, S Ghosh, CV Rothlin, C Patsch, B Geering
Cell Death & Disease, 2021-05-25;12(6):538.
Species: Human
Sample Types: Protein
Applications: SPR
TREM2 Is a Receptor for ?-Amyloid that Mediates Microglial Function
Authors: Y Zhao, X Wu, X Li, LL Jiang, X Gui, Y Liu, Y Sun, B Zhu, JC Piña-Cresp, M Zhang, N Zhang, X Chen, G Bu, Z An, TY Huang, H Xu
Neuron, 2018-03-07;97(5):1023-1031.e7.
Species: Human
Sample Types: Recombinant Protein
Applications: Immunoprecipitation
Efficient clearance of early apoptotic cells by human macrophages requires M2c polarization and MerTK induction.
Authors: Zizzo G, Hilliard B, Monestier M, Cohen P
J Immunol, 2012-08-31;189(7):3508-20.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay
Targeting Axl with an high-affinity inhibitory aptamer.
Authors: Cerchia, Laura, Esposito, Carla L, Camorani, Simona, Rienzo, Anna, Stasio, Loredana, Insabato, Luigi, Affuso, Andrea, de Franciscis, Vittorio
Mol Ther, 2012-08-21;20(12):2291-303.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay
Auto-oxidation and oligomerization of protein S on the apoptotic cell surface is required for mer tyrosine kinase-mediated phagocytosis of apoptotic cells.
Authors: Uehara H, Shacter E
J. Immunol., 2008-02-15;180(4):2522-30.
Species: Human
Sample Types: Phospholipid Vesicles
Applications: Bioassay