Recombinant Human Mesothelin (aa 35-289) His-tag Protein, CF

Catalog #: 10093-MS Datasheet / COA / SDS

N-Terminal/MPF

Catalog #	Availability	Size / Price	Qty
10093-MS-050

Recombinant Human Mesothelin (aa 35-289) His-tag Protein SDS-PAGE

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Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Human Mesothelin (aa 35-289) His-tag Protein, CF Summary

Product Specifications

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

Activity

Bioassay data are not available.

Source

Mouse myeloma cell line, NS0-derived human Mesothelin protein
Arg35-Leu289, with a C-terminal 6-His tag

Accession #

Q13421

N-terminal Sequence
Analysis

Arg35

Structure / Form

Monomer

Predicted Molecular Mass

28 kDa

SDS-PAGE

27-39 kDa, reducing conditions

Product Datasheets

10093-MS

Product Datasheet

COA

SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

10093-MS

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution	Reconstitute at 500 μg/mL in PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Scientific Data

SDS-PAGE

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2 μg/lane of Recombinant Human Mesothelin N-Terminal (aa 35-289) His-tag (Catalog# 10093-MS) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 27-39 kDa.

Reconstitution Calculator

Background: Mesothelin

Megakaryocyte-Potentiating Factor (MPF), is derived from a 70 kDa Mesothelin precursor (1-3). The 70 kDa precursor is expressed on the cell surface where it is cleaved at a dibasic furin cleavage site between aa 288-293. The cleavage releases soluble N-terminal MPF (aa 37-286) and C-terminal Mesothelin (aa 296-606) remains attached to the cell surface via a GPI linkage. (3, 4). MPF is a cytokine that potentiates IL-3 induced megakaryocyte colony formation (2, 5). This recombinant human MPF shares 56% sequence identity with mouse and rat MPF. Apart from its role as a megakaryocyte potentiating cytokine, MPF may have the potential to serve as a biomarker for detection and monitoring of malignant mesothelioma and related cancers (6).

References

Hassan, R. et al. (2004) Clin. Cancer Res. 10:3937.
Kojima, T. et al. (1995) J. Biol. Chem. 270:21984.
Chang, K. and I. Pastan (1996) Proc. Natl. Acad. Sci. 93:136.
Onda, M. et al. (2006) Clin. Cancer Res. 12:4225.
Yamaguchi, N. et al. (1994) J. Biol. Chem. 269:805.
Raiko, I. et al. (2017) Biochem. Biophy. Res. Com. 486:526.

Entrez Gene IDs

10232 (Human); 56047 (Mouse); 60333 (Rat); 102130460 (Cynomolgus Monkey)

Alternate Names

CAK1 antigen; CAK1; megakaryocyte potentiating factor; Mesothelin; MPF; MPFSMRP; MSLN; Pre-pro-megakaryocyte-potentiating factor; SMR; soluble MPF mesothelin related protein