Recombinant Human MSPR/Ron His-tag Avi-tag Protein, CF

Catalog #: AVI1947 Datasheet / COA / SDS

Biotinylated

Catalog #	Availability	Size / Price	Qty
AVI1947-050

Recombinant Human MSPR/Ron His-tag Avi-tag Protein Binding Activity

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Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Human MSPR/Ron His-tag Avi-tag Protein, CF Summary

Learn more about Avi-tag Biotinylated Proteins

Product Specifications

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

Activity

Measured by its binding ability in a functional ELISA. When Recombinant Human MSP/MST1 (Cys672Ala) (Catalog # 4306-MS) is immobilized at 0.5 μg/mL, 100 μL/well, the concentration of Biotinylated Recombinant Human MSPR/Ron His-tag Avi-tag that produces 50% of the optimal binding response is approximately 40‑240 ng/mL.

Source

Human embryonic kidney cell, HEK293-derived human MSPR/Ron protein

Human MSPR/Ron (Glu25-Leu571) Accession # CAA49634.1	HHHHHH	Avi-tag
N-terminus		C-terminus

Accession #

CAA49634.1

N-terminal Sequence
Analysis

Glu25 ( alpha chain) and Gly310 ( beta chain)

Structure / Form

Biotinylated via Avi-tag

Predicted Molecular Mass

30 kDa ( alpha chain) & 31 kDa ( beta chain)

SDS-PAGE

34-42 kDa & 64-78 kDa, under reducing conditions.

Product Datasheets

AVI1947

Product Datasheet

COA

SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

AVI1947

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution	Reconstitute at 200 μg/mL in PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Scientific Data

Binding Activity

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When Recombinant Human MSP/MST1 (Cys672Ala) Protein (Catalog # 4306-MS) is immobilized at 0.5 µg/mL, 100 µL/well, the concentration of Biotinylated Recombinant Human MSPR/Ron His-tag Avi-tag that produces 50% of the optimal binding response is approximately 40-240 ng/mL.

SDS-PAGE

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2 μg/lane of Recombinant Human MSPR/Ron His-tag Avi-tag Protein (Catalog # AVI1947) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 34-42 and 64-78 kDa.

Reconstitution Calculator

Background: MSPR/Ron

Macrophage stimulating protein receptor (MSPR), encoded by the human Ron and the mouse Stk, is one of a small family of receptor tyrosine kinases (RTKs) that also includes human Met (the receptor for hepatocyte growth factor) and chicken Sea (1, 2). This family of receptors is synthesized as a single-chain precursors that are cleaved into a mature disulfide-linked heterodimers composed of an extracellular alpha chain and a membrane spanning beta chain with intrinsic tyrosine kinase activity. Biologically active ligands (MSP and HGF) for this family of receptors are also disulfide-linked alpha -beta heterodimers. Human MSPR cDNA encodes a 1400 amino acid (aa) residue precursor protein with a 24 aa signal peptide, a 285 aa residue alpha chain (Glu25-Arg309) and a 1091 aa residue transmembrane beta chain (Gly310-Thr1400). The extracellular domain of MSPR is comprised of an N-terminal sema domain, a PSI (plexin semaphorins integrins) domain, followed by four immunoglobulin-like folds shared by plexins and transcription factors (3). The soluble sema domain binds MSP and inhibits the MSPR-dependent signaling pathways. MSP receptor is expressed in multiple tissues including specific areas of the central and peripheral nervous systems, epithelial cells along the digestive tract, skin and lung, and in subpopulations of the mononuclear phagocyte lineage (1, 2). Although free MSP alpha or beta chains have been shown to bind MSPR, only the heterodimeric MSP can induce receptor activation and cause biological activity (4, 5). MSPR associates with other transmembrane molecules including integrins, cadherins and other cytokine receptors. Transactivation and signaling crosstalk between MSPR and its associated transmembrane receptors have been demonstrated (6-8). Human MSPR shares 72% amino acid sequence identity with mouse MSPR.

References

Gaudino, G. et al. (1994) EMBO J. 13:3524.
Wang, M-H. et al. (1994) Science 266:117.
Angelonis, D. et al. (2004) J. Biol. Chem. 279:3726.
Wang, M-H. et al. (1997) J. Biol. Chem. 272:16999.
Danilkovitch, A. et al. (1999) J. Biol. Chem. 274:29937.
Danilkovitch-Miagkova, A. et al. (2000) J. Biol. Chem. 275:14783.
Danilkovitch-Miagkova, A. and A. Leonard (2001) Histol. Histopathol. 16:623.
Santora, M. et al. (2003) Devel. Cell 2:257.

Long Name

Macrophage Stimulating Protein Receptor

Entrez Gene IDs

4486 (Human); 19882 (Mouse)

Alternate Names

CD136 antigen; CD136; CDw136c-met-related tyrosine kinase; EC 2.7.10; EC 2.7.10.1; macrophage stimulating 1 receptor (c-met-related tyrosine kinase); MSP R; MSP receptor; MSPR; MST1R; p185-Ron; Protein-tyrosine kinase 8; PTK8 protein tyrosine kinase 8; PTK8; Ron; RONmacrophage-stimulating protein receptor; soluble RON variant 1; soluble RON variant 2; soluble RON variant 3; soluble RON variant 4