Recombinant Human NF-L Protein, CF

Catalog #: 9715-NF Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
9715-NF-050

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Summary Product Datasheets Carrier Free Reconstitution Calculator Background Related Research Areas

Recombinant Human NF-L Protein, CF Summary

Product Specifications

Purity

>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

Activity

Bioassay data are not available.

Source

E. coli-derived human NF-L protein
Ser2-Asp338, with a C-terminal 6-His tag

Accession #

P07196

N-terminal Sequence
Analysis

Ser2

Predicted Molecular Mass

39 kDa

SDS-PAGE

41 kDa, reducing conditions

Product Datasheets

9715-NF

Product Datasheet

COA

SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

9715-NF

Formulation	Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA.
Reconstitution	Reconstitute at 500 μg/mL in 4 mM HCl..
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Reconstitution Calculator

Background: NF-L

Neurofilament light polypeptide (NF-L), also known as 68 kDa neurofilament protein, and neurofilament triplet L protein, is an approximately 68 kDa member of the neuronal intermediate filament family that plays a role in the maintenance of neuronal caliber and possibly the pathogenesis of motor neuron diseases (1, 2). Out of the three intermediate filament proteins (NF-L, NF-M, NF-H), NF-L is the most abundant. NF-L is a 542 amino acid (aa) cytoskeletal protein which consists of a 91 aa N-terminal head, a 304 aa alpha -helical rod, and a 147 aa C-terminal tail (3). Within the extracellular domains, human NF-L shares 95% and 96% sequence identity with mouse and rat NF-L, respectively.

References

Monteiro, MJ. et al. (1990) J. Cell. Biol. 111:1543.
Xu, Z. et al. (1993) Cell. 73:23.
Adebola, A. et al. (2015) Hum. Mol. Genet. 24:2163.

Long Name

Neurofilament Protein, Light Chain

Entrez Gene IDs

4747 (Human)

Alternate Names

68 kDa neurofilament protein; CMT2E; NEFL; neurofilament protein, light chain; neurofilament subunit NF-L; Neurofilament triplet L protein; neurofilament, light polypeptide; neurofilament-light; NF68; NF68FLJ53642; NFL; NF-L; NFLlight polypeptide 68kDa