Recombinant Human NGFR/TNFRSF16 Fc Chimera Protein

Catalog #: 367-NR
11 Citations Datasheet / COA / SDS

Carrier Free

Catalog #	Availability	Size / Price	Qty
367-NR-050/CF

With Carrier

Catalog #	Availability	Size / Price	Qty
367-NR-050

R&D Systems Recombinant Proteins and Enzymes

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Product Details

Citations (11)

Reviews

Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Human NGFR/TNFRSF16 Fc Chimera Protein Summary

Product Specifications

Purity

>95%, by SDS-PAGE under reducing conditions and visualized by silver stain

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

Activity

Measured by its ability to inhibit beta -NGF-dependent proliferation of TF‑1 human erythroleukemic cells. Kitamura, T. et al. (1989) J. Cell Physiol. 140:323. The ED₅₀ for this effect is 0.2-0.6 µg/mL.

Source

Spodoptera frugiperda, Sf 21 (baculovirus)-derived human NGF R/TNFRSF16 protein

Human NGF R Lys29-Asn250 Accession # P08138	DIEGRMD	Human IgG₁ (Pro100-Lys330)	6-His tag
N-terminus			C-terminus

Accession #

P08138

N-terminal Sequence
Analysis

Lys29

Structure / Form

Disulfide-linked homodimer

Predicted Molecular Mass

51 kDa (monomer)

SDS-PAGE

70-80 kDa, reducing conditions

Product Datasheets

367-NR (with carrier)

Product Datasheet

COA

SDS

367-NR/CF (carrier free)

Product Datasheet

COA

SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

367-NR

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Reconstitution	Reconstitute at 50 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

367-NR/CF

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution	Reconstitute at 100 μg/mL in sterile PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Reconstitution Calculator

Background: NGFR/TNFRSF16

p75 neurotrophin receptor, also named low affinity NGF receptor (NGF R), is a type I transmembrane protein that belongs to the tumor necrosis factor receptor family. NGF R cDNA encodes a 427 amino acid (aa) residue precursor protein with a 28 aa residue signal peptide, a 222 aa residue extracellular domain, a 22 aa residue transmembrane domain and a 155 aa residue intracellular domain. The extracellular region contains four cysteine-rich domains and binds NGF, BDNF, NT-3 and NT-4 approximately equally with low affinity. The cytoplasmic region contains a subtype 2 death domain.

NGF R expression has been shown to occur widely during development and in the adult. Expression has been detected in both neuronal and non-neuronal cells. NGF R was originally reported to function as a positive regulator of TrkA activity. NGF R has also been shown to signal by itself. Depending on its cellular environment, NGF R has now been shown to regulate cell migration, gene expression and to mediate apoptosis. Recombinant NGF R/Fc chimera binds NGF with high affinity and is a potent NGF antagonist. Naturally occurring truncated NGF R containing the extracellular domain and lacking the transmembrane or intracellular domain has been detected in vivo in urine, plasma and in amniotic fluid of humans and rats.

References

Barker, P.A. and R.A. Murphy (1992) Molecular and Cellular Biochemistry 110:1.
Bamji, A.X. et al. (1998) J. Cell Biol. 140:911.
Feinstein, E. et al. (1995) Trends Biochem. Sci. 20:342.

Long Name

Nerve Growth Factor Receptor

Entrez Gene IDs

4804 (Human); 18053 (Mouse); 117089 (Rat)

Alternate Names

CD271 antigen; CD271; Gp80-LNGFR; member 16; nerve growth factor receptor; NGF R; NGF receptor; NGFR; p75 ICD; p75 NTR; TNFRSF16; TNFRSF16nerve growth factor receptor (TNFR superfamily, member 16); tumor necrosis factor receptor superfamily member 16

Related Research Areas

Citations for Recombinant Human NGFR/TNFRSF16 Fc Chimera Protein

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

11 Citations: Showing 1 - 10
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Prognostic clinical and biological markers for amyotrophic lateral sclerosis disease progression: validation and implications for clinical trial design and analysis
Authors: Benatar, M;Macklin, EA;Malaspina, A;Rogers, ML;Hornstein, E;Lombardi, V;Renfrey, D;Shepheard, S;Magen, I;Cohen, Y;Granit, V;Statland, JM;Heckmann, JM;Rademakers, R;McHutchison, CA;Petrucelli, L;McMillan, CT;Wuu, J;CReATe Consortium PGB1 Study Investigators, ;
EBioMedicine
Species: Human
Sample Types: Recombinant Protein
Applications: ELISA Standard
Prognostic Clinical and Biological Markers for Amyotrophic Lateral Sclerosis Disease Progression: Validation and Implications for Clinical Trial Design and Analysis.
Authors: Benatar, M;Macklin, EA;Malaspina, A;Rogers, ML;Hornstein, E;Lombardi, V;Renfrey, D;Shepheard, S;Magen, I;Cohen, Y;Granit, V;Statland, JM;Heckmann, JM;Rademakers, R;McHutchison, CA;Petrucelli, L;McMillan, CT;Wuu, J;
medRxiv : the preprint server for health sciences
Species: Human
Sample Types: Recombinant Protein
Applications: ELISA Standard
Vemurafenib induces a noncanonical senescence-associated secretory phenotype in melanoma cells which promotes vemurafenib resistance
Authors: Peng, J;Lin, Z;Chen, W;Ruan, J;Deng, F;Yao, L;Rao, M;Xiong, X;Xu, S;Zhang, X;Liu, X;Sun, X;
Heliyon
Species: Human
Sample Types: Cell Culture Supernates
Applications: Bioassay
Urinary Extracellular Domain of Neurotrophin Receptor p75 as a Biomarker for Amyotrophic Lateral Sclerosis in a Chinese cohort
Authors: R Jia, S Shepheard, J Jin, F Hu, X Zhao, L Xue, L Xiang, H Qi, Q Qu, F Guo, ML Rogers, J Dang
Sci Rep, 2017-07-11;7(1):5127.
Species: Human
Sample Types: Urine
Applications: ELISA (Standard)
Urinary p75(ECD): A prognostic, disease progression, and pharmacodynamic biomarker in ALS
Authors: SR Shepheard, J Wuu, M Cardoso, L Wiklendt, PG Dinning, T Chataway, D Schultz, M Benatar, ML Rogers
Neurology, 2017-02-22;88(12):1137-1143.
Species: Human
Sample Types: Urine
Applications: ELISA (Standard)
Discovery of a junctional epitope antibody that stabilizes IL-6 and gp80 protein:protein interaction and modulates its downstream signaling
Authors: R Adams, RJ Burnley, CR Valenzano, O Qureshi, C Doyle, S Lumb, M Del Carmen, R Griffin, D McMillan, RD Taylor, C Meier, P Mori, LM Griffin, U Wernery, J Kinne, S Rapecki, TS Baker, AD Lawson, M Wright, A Ettorre
Sci Rep, 2017-01-30;7(0):37716.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay
Inhibitory Effects of Polyacetylene Compounds from Panax ginseng on Neurotrophin Receptor-Mediated Hair Growth
Authors: A Suzuki, D Matsuura, H Kanatani, S Yano, M Tsunakawa, S Matsuyama, H Shigemori
Biol. Pharm. Bull., 2017-01-01;40(10):1784-1788.
Species: Human
Sample Types: Protein
Applications: Bioassay
The extracellular domain of neurotrophin receptor p75 as a candidate biomarker for amyotrophic lateral sclerosis.
Authors: Shepheard S, Chataway T, Schultz D, Rush R, Rogers M
PLoS ONE, 2014-01-27;9(1):e87398.
Species: Human
Sample Types: Urine
Applications: ELISA (Standard)
Peptides derived from the solvent-exposed loops 3 and 4 of BDNF bind TrkB and p75(NTR) receptors and stimulate neurite outgrowth and survival.
Authors: Fobian K, Owczarek S, Budtz C
J. Neurosci. Res., 2010-05-01;88(6):1170-81.
Species: Human
Sample Types: Recombinant Protein
Applications: Surface Plasmon Resonance
A novel immunoprecipitation strategy identifies a unique functional mimic of the glial cell line-derived neurotrophic factor family ligands in the pathogen Trypanosoma cruzi.
Authors: Lu B, PereiraPerrin M
Infect. Immun., 2008-06-09;76(8):3530-8.
Species: Bacteria
Sample Types: Trypanosoma
Applications: PcIP

Suppression of p75NTR does not promote regeneration of injured spinal cord in mice.
Authors: Song XY, Zhong JH, Wang X, Zhou XF
J. Neurosci., 2004-01-14;24(2):542-6.
Species: Mouse
Sample Types: In Vivo
Applications: In Vivo