Recombinant Human NPDC-1 Protein, CF

Catalog #: 9724-NP Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
9724-NP-050

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Summary Product Datasheets Carrier Free Reconstitution Calculator Background Related Research Areas

Recombinant Human NPDC-1 Protein, CF Summary

Product Specifications

Purity

>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

Activity

Bioassay data are not available.

Source

Mouse myeloma cell line, NS0-derived human NPDC-1 protein
Gly35-Asp181, with a C-terminal 6-His tag

Accession #

Q9NQX5

N-terminal Sequence
Analysis

Gly35

Predicted Molecular Mass

16 kDa

SDS-PAGE

25 - 39 Kda, reducing conditions

Product Datasheets

9724-NP

Product Datasheet

COA

SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

9724-NP

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution	Reconstitute at 250 μg/mL in PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Reconstitution Calculator

Background: NPDC-1

Neural proliferation and differentiation control protein 1 (NPDC1) is an approximately 34 kDa protein that is mainly expressed in lung and neural tissues and whose expression is developmentally regulated and persists in the adult (1, 2). It binds a variety of cyclins and regulate differentiation events in neuronal precursor cells (3). NPDC1 also binds the transcription factor, E2F-1, which results in the inhibition of E2F-1 binding to DNA and the corresponding transcription events (4). The encoded protein of the human NPDC1 includes a 34 aa signal peptide, a 147 aa extracellular domain (ECD), a 21 aa transmembrane region, followed by a carboxyl terminus containing a PEST motif (rich in proline, glutamine, serine, and threonine) which targets the protein for degradation (1). Alignment of the mature protein shows that human NPDC1 shares 76% and 75% sequence identity with mouse and rat NPDC1, respectively.

References

Spencer, M.L. et al. (2004) J. Biol. Chem. 279:37069.
Evrard, C. and P. Rouget (2005) J. Neurosci. Res. 79:747.
Galiana, E. et al. (1995) Proc. Natl. Acad. Sci. USA. 92:1560.
Sansal, I. et al. (2000) Oncogene 19:5000.

Long Name

Neural Proliferation, Differentiation and Control, 1

Entrez Gene IDs

56654 (Human); 18146 (Mouse); 296562 (Rat)

Alternate Names

CAB-; CAB1; CAB-1; CAB1CAB; DKFZp586J0523; neural proliferation differentiation and control protein 1; neural proliferation, differentiation and control, 1; NPDC1; NPDC-1