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Recombinant Human PDGF R alpha Protein

Catalog #: 322-PR
4 Citations Datasheet / COA / SDS
Newer Version Available: 322-PR/CF
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Discontinued Product

322-PR has been discontinued and is replaced by 322-PR/CF.

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Citations (4)
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Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Human PDGF R alpha Protein Summary

Product Specifications

Purity
>97%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Level
<1.0 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its binding ability in a functional ELISA. When Recombinant Human PDGF R alpha is immobilized at 2 µg/mL (100 µL/well), Recombinant Human PDGF-AB (Catalog # 222-AB) binds with an ED50 of 2-12 ng/mL.
Source
Mouse myeloma cell line, NS0-derived human PDGF R alpha protein
Gln24-Glu524
Accession #
P16234.1
N-terminal Sequence
Analysis
No results obtained: Gln24 predicted
Predicted Molecular Mass
56 kDa
SDS-PAGE
95-107 kDa, reducing conditions

Product Datasheets

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322-PR (with carrier)

Product Datasheet
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SDS

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322-PR/CF (carrier free)

Product Datasheet
COA
SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

322-PR

Formulation Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose and with BSA as a carrier protein.
Reconstitution Reconstitute at 50 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

322-PR/CF

Formulation Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: PDGF R alpha

PDGF R alpha (platelet-derived growth factor receptor alpha) is a type I transmembrane glycoprotein in the class III subfamily of receptor tyrosine kinases (RTK) (1‑4). PDGF R alpha and PDGF R beta can form homo- or hetero-dimeric receptors when engaged by dimers of the PDGF family of growth factors, which include disulfide-linked homodimers of PDGF-A, B, C or D, or the heterodimer PDGF-AB that is mainly found in human platelets. While multiple in vitro ligand-receptor combinations have been identified, in vivo evidence indicates that PDGF R alpha primarily binds PDGF-AA and PDGF-CC, while PDGF R beta primarily binds PDGF-BB and probably PDGF-DD. Like all class III RTKs, the extracellular domain (ECD) of human PDGF R alpha (aa 24‑524) contains five immunoglobulin-like domains, while the intracellular region contains a split tyrosine kinase domain (aa 593‑954) (1‑4). Within the ECD, human PDGF R alpha shares 85%, 83%, 95%, 93%, and 88% aa sequence identity with mouse, rat, equine, canine and bovine PDGF R alpha  respectively. PDGF R alpha autophosphorylates upon dimerization, activating signaling cascades in PI 3-kinase Ras-MAP kinase, and PLC-gamma pathways (1, 2). Signaling is down‑regulated by SHP-2 phosphatase activity and by receptor endocytosis and lysosomal degradation. PDGF R alpha is expressed at low levels in most mesenchymal cells, but is strongly expressed in oligodendrocyte, lung, skin and intestinal progenitor cells and induced by inflammation or growth in culture (1‑4). During development, mesenchymal cells expressing PDGF R alpha respond to local gradients of epithelially produced PDGF-AA or PDGF-CC during formation of the cranial and cardiac neural crest, retina, gonads, lung alveoli, intestinal villi, skin, hair follicles, skeleton, teeth, palate, and interstitial kidney mesenchyme (1, 5). Deletion of PDGF R alpha in mice severely impairs mesenchymal derivatives in both embryo and extraembryonic tissues, and high or low PDGF R alpha signaling in humans may result in spina bifida or cleft palate‑type malformations. Postnatally, PDGF R alpha is implicated in gliomas and fibrotic disorders of lung, heart and skin (scleroderma) (6‑8).

References
  1. Andrae, J. et al. (2008) Genes Dev. 22:1276.
  2. Heldin, C-H. and B. Westermark (1999) Physiol. Rev. 79:1283.
  3. Claesson-Welsh, L. et al. (1989) Proc. Natl. Acad. Sci. USA 86:4917.
  4. Matsui, T. et al. (1989) Science 243:800.
  5. Klinghoffer, R.A. et al. (2002) Dev. Cell 2:103.
  6. Martinho, O. (2009) Br. J. Cancer 101:973.
  7. Olson, L.E. and P. Soriano (2009) Dev. Cell 16:303.
  8. Baroni, S.S. et al. (2006) N. Engl. J. Med. 354:2667.
Long Name
Platelet-derived Growth Factor Receptor alpha
Entrez Gene IDs
5156 (Human); 18595 (Mouse)
Alternate Names
alpha-type platelet-derived growth factor receptor; CD140 antigen-like family member A; CD140a antigen; CD140a; EC 2.7.10; EC 2.7.10.1; MGC74795; PDGF R alpha; PDGFR alpha; PDGFR2; PDGFRA; PDGFRA/BCR fusion; PDGF-R-alpha; platelet-derived growth factor receptor, alpha polypeptide; rearranged-in-hypereosinophilia-platelet derived growth factor receptor alphafusion protein; RHEPDGFRA

Citations for Recombinant Human PDGF R alpha Protein

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

4 Citations: Showing 1 - 4
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  1. Fibroblast, Epithelial and Endothelial Cell-Derived Human Cytomegalovirus Strains Display Distinct Neutralizing Antibody Responses and Varying Levels of gH/gL Complexes
    Authors: C Fornara, E Schultz, D Lilleri, F Baldanti, B Ryckman, G Gerna
    International Journal of Molecular Sciences, 2023-02-23;24(5):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  2. Discovery of High-Affinity PDGF-VEGFR Interactions: Redefining RTK Dynamics
    Authors: SB Mamer, S Chen, JC Weddell, A Palasz, A Wittenkell, M Kumar, PI Imoukhuede
    Sci Rep, 2017-11-27;7(1):16439.
    Species: Human
    Sample Types: Recombinant Protein
    Applications: Surface Plasmon Resonance
  3. Binding of CD157 protein to fibronectin regulates cell adhesion and spreading.
    Authors: Morone S, Augeri S, Cuccioloni M, Mozzicafreddo M, Angeletti M, Lo Buono N, Giacomino A, Ortolan E, Funaro A
    J Biol Chem, 2014-04-21;289(22):15588-601.
    Species: Human
    Sample Types: Protein
    Applications: Bioassay
  4. Inhibition of receptor signaling and of glioblastoma-derived tumor growth by a novel PDGFRbeta aptamer.
    Authors: Camorani S, Esposito C, Rienzo A, Catuogno S, Iaboni M, Condorelli G, de Franciscis V, Cerchia L
    Mol Ther, 2014-01-02;22(4):828-41.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay

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