Recombinant Human Ret His-tag Protein, CF

Catalog #: 10302-CR Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
10302-CR-050

Recombinant Human Ret His-tag Protein Binding Activity

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Product Details

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Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Human Ret His-tag Protein, CF Summary

Product Specifications

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

Activity

Measured by its binding ability in a functional ELISA. When Recombinant Human Ret His-tag (Catalog # 10302-CR) is immobilized at 1 µg/mL (100 µL/well), Recombinant Human GDNF (Catalog # 212-GD) binds with an ED₅₀ of 0.03-0.3 μg/mL in the presence of Recombinant Human GFR alpha ‑1/GDNF R alpha‑1 Fc Chimera (Catalog # 714-GR).

Source

Mouse myeloma cell line, NS0-derived human Ret protein
Leu29-Arg635, with a C-terminal 10-His tag

Accession #

P07949

N-terminal Sequence
Analysis

Leu29

Predicted Molecular Mass

69 kDa

SDS-PAGE

101-113 kDa, under reducing conditions

Product Datasheets

10302-CR

Product Datasheet

COA

SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

10302-CR

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution	Reconstitute at 500 μg/mL in PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Scientific Data

Binding Activity

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When Recombinant Human Ret His-tag (Catalog # 10302-CR) is immobilized at 1 µg/mL, 100 µL/well, Recombinant Human GDNF (Catalog # 212-GD) binds with an ED₅₀ of 0.03-0.3 µg/mL in the presence of Recombinant Human GFR alpha-1 Fc Chimera (Catalog # 714-GR).

SDS-PAGE

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2 μg/lane of Recombinant Human Ret His-tag (Catalog # 10302-CR) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 101-113 kDa.

Reconstitution Calculator

Background: Ret

Receptor tyrosine-protein kinase, also known as Ret, is encoded by the C-RET proto-oncogene. Mutations of the C-RET gene have been associated with various human diseases affecting tissues derived from the neural crest, including Hirschsprung's disease, multiple endocrine neoplasia MEN2A and MEN2B, and familial medullary thyroid carcinoma (1, 2, 3). Human C-RET cDNA encodes a 1115 amino acid (aa) with a 28 aa signal peptide, a 679 aa cysteine-rich extracellular domain that contains an membrane anchored Ret-Cadherin 120 Da fragment, and a 456 aa rcytoplasmic domain with a soluble Ret kinase fragment (4). Human and mouse Ret share 83% amino acid sequence homology (77% homology in the extracellular domain and 93% homology in the cytoplasmic domain). Although Ret does not bind GDNF ligands directly, the extracellular domain of Ret binds the GDNF-GFR-alpha complex with high affinity and is a potent GDNF antagonist in the presence of soluble GFR-alpha (5).

References

Ma, Q. (2009) Neuron 64:773.
Carlomagno, F. et al. (1998) Endocrinology 139:3613.
Cabrera, J.R. et al. (2011) J. Biol. Chem. 286:14628.
Iwamoto, T. et al. (1993) Oncogene 8:1087.
Yang, L. et al. (2017) Nat. Med. 23:1158.

Long Name

Proto-oncogene Tyrosine-protein Kinase Receptor Ret

Entrez Gene IDs

5979 (Human); 19713 (Mouse)

Alternate Names

CDHF12MTC1; CDHR16; EC 2.7.10; Hirschsprung disease 1; Hirschsprung disease); MEN2A; MEN2B; multiple endocrine neoplasia and medullary thyroid carcinoma 1; ret proto-oncogene; RET transforming sequence; Ret