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Recombinant Human Ret His-tag Protein

Catalog #: 1168-CR
2 Citations Datasheet / COA / SDS
Newer Version Available: 10302-CR
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Discontinued Product

1168-CR has been discontinued and is replaced by 10302-CR.

Recombinant Human Ret Protein Binding Activity
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Product Details
Citations (2)
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Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Human Ret His-tag Protein Summary

Product Specifications

Purity
>90%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its binding ability in a functional ELISA.

When Recombinant Human Ret His-tag is immobilized at 1 µg/mL (100 µL/well), Recombinant Human GDNF (Catalog # 212-GD) binds with an ED50 of 0.05-0.3 μg/mL in the presence of Recombinant Human GFR alpha‑1/GDNF R alpha‑1 Fc Chimera (Catalog # 714-GR).

Source
Spodoptera frugiperda, Sf 21 (stably transfected)-derived human Ret protein
Leu29-Arg635, with a C-terminal 10-His tag
Accession #
P07949
N-terminal Sequence
Analysis
Leu29
Predicted Molecular Mass
69 kDa
SDS-PAGE
90 kDa, reducing conditions

Product Datasheets

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1168-CR (with carrier)

Product Datasheet
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1168-CR/CF (carrier free)

Discontinued Product

1168-CR

Formulation Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Scientific Data

Binding Activity Recombinant Human Ret Protein Binding Activity View Larger

When Recombinant Human Ret His-tag (Catalog # 1168-CR) is immobilized at 1 μg/mL (100 μL/well), Recombinant Human GDNF (Catalog # 212-GD) binds with an ED50 of 0.05-0.3 μg/mL in the presence of Recombinant Human GFR alpha‑1/GDNF R alpha‑1 Fc Chimera (Catalog # 714-GR).

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Ret

The GDNF family of neurotrophic factors forms a subfamily within the TGF-beta superfamily. These proteins are potent survival factors for various central and peripheral neurons during development and the adult animal. The GDNF family members (GDNF, neurturin, artemin and persephin) signal through multicomponent receptors that consist of the Ret receptor tyrosine kinase and one of four glycosyl-phosphatidylinositol (GPI)-linked ligand-binding subunits (GFR alpha -1 - 4). GFR alpha -1 -2, -3 and -4 are the preferred ligand-binding subunits for GDNF, neurturin, artemin and persephin, respectively. The Ret tyrosine-kinase receptor is encoded by the c-ret proto-oncogene. Mutations of the ret gene have been associated with various human diseases affecting tissues derived from the neural crest, including Hirschsprung’s disease, multiple endocrine neoplasia MEN2A and MEN2B, and familial medullary thyroid carcinoma. Human and mouse Ret share 83% amino acid sequence homology (77% homology in the extracellular domain and 93% homology in the cytoplasmic domain). Although Ret does not bind GDNF ligands directly, the extracellular domain of Ret binds the GDNF-GFR-alpha complex with high affinity and is a potent GDNF antagonist in the presence of soluble GFR-alpha (1 - 4).

References
  1. Trupp, M. et al. (1998) Mol. Cell. Neurosci. 11:47.
  2. Enokido, Y. et al. (1998) Curr. Biol. 8:1019.
  3. Carlomagno, F. et al. (1998) Endocrinology 139:3613.
  4. Baloh, R. et al. (1998) Neuron 21:1291.
Long Name
Proto-oncogene Tyrosine-protein Kinase Receptor Ret
Entrez Gene IDs
5979 (Human); 19713 (Mouse)
Alternate Names
CDHF12MTC1; CDHR16; EC 2.7.10; Hirschsprung disease 1; Hirschsprung disease); MEN2A; MEN2B; multiple endocrine neoplasia and medullary thyroid carcinoma 1; ret proto-oncogene; RET transforming sequence; Ret

Citations for Recombinant Human Ret His-tag Protein

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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  1. Long-term post-mortem studies following neurturin gene therapy in patients with advanced Parkinson's disease.
    Authors: Chu Y, Bartus R, Manfredsson F, Olanow C, Kordower J
    Brain, 2020-03-01;143(3):960-975.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: Adsorption
  2. A novel immunoprecipitation strategy identifies a unique functional mimic of the glial cell line-derived neurotrophic factor family ligands in the pathogen Trypanosoma cruzi.
    Authors: Lu B, PereiraPerrin M
    Infect. Immun., 2008-06-09;76(8):3530-8.
    Species: Bacteria
    Sample Types: Trypanosoma
    Applications: PcIP

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