Recombinant Human Siglec-7/CD328 Fc Chimera Protein, CF

Catalog #: 1138-SL
8 Citations Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
1138-SL-050

Best Seller

R&D Systems Recombinant Proteins and Enzymes

1 Image

Product Details

Citations (8)

Supplemental Products

Reviews

Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Human Siglec-7/CD328 Fc Chimera Protein, CF Summary

Product Specifications

Purity

>85%, by SDS-PAGE under reducing conditions and visualized by silver stain

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

Activity

Measured by the ability of the immobilized protein to support the adhesion of human red blood cells. Kelm, S. et al. (1994) Current Biology 4:965. The ED₅₀ for this effect is 0.5‑2 µg/mL.

Source

Mouse myeloma cell line, NS0-derived human Siglec-7/CD328 protein

Human Siglec-7 (Gln19-Gly357) Accession # Q9Y286	DIEGRMD	Human IgG₁ (Pro100-Lys330)
N-terminus		C-terminus

Accession #

Q9Y286

N-terminal Sequence
Analysis

No results obtained: Gln19 predicted

Structure / Form

Disulfide-linked homodimer

Predicted Molecular Mass

64 kDa (monomer)

SDS-PAGE

90-100 kDa, reducing conditions

Product Datasheets

1138-SL

Product Datasheet

COA

SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

1138-SL

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS and NaCl.
Reconstitution	Reconstitute at 100 μg/mL in sterile PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Reconstitution Calculator

Background: Siglec-7/CD328

Siglecs (1) (sialic acid binding Ig-like lectins) are I-type (Ig-type) lectins belonging to the Ig superfamily. They are characterized by an N-terminal Ig-like V-type domain which mediates sialic acid binding, followed by varying numbers of Ig-like C2-type domains (1, 2). Eleven human Siglecs have been cloned and characterized. They are sialoadhesin/CD169/Siglec-1, CD22/Siglec-2, CD33/Siglec-3, Myelin-Associated Glycoprotein (MAG/Siglec-4a) and Siglecs 5 to 11 (1 - 4). To date, no Siglec has been shown to recognized any cell surface ligand other than sialic acids, suggesting that interactions with glycans containing this carbohydrate are important in mediating the biological functions of Siglecs. Siglecs 5 to 11 share a high degree of sequence similarity with CD33/Siglec-3 both in their extracellular and intracellular regions. They are collectively referred to as CD33-related Siglecs. One remarkable feature of the CD33-related Siglecs is their differential expression pattern within the hematopoietic system (2, 3). This fact, together with the presence of two conserved immunoreceptor tyrosine-based inhibition motifs (ITIMs) in their cytoplasmic tails, suggests that CD33-related Siglecs are involved in the regulation of cellular activation within the immune system.

Human Siglec-7 encodes a 467 amino acid (aa) polypeptide with a hydrophobic signal peptide, an N-terminal Ig-like V-type domain, two Ig-like C2-type domains, a transmembrane region and a cytoplasmic tail (5). Siglec-7 exists as a monomer on the cell surface and is expressed on natural killer cells, CD8⁺ T cells and monocytes (3, 5). It binds equally well to both alpha 2,3- and alpha 2,6-linked sialic acid (5). The gene encoding Siglec-7 was mapped to chromosome 19q13.3.

References

Crocker, P.R. et al. (1998) Glycobiology 8:v.
Crocker, P.R. and A. Varki (2001) Trends Immunol. 22:337.
Crocker, P.R. and A. Varki (2001) Immunology 103:137.
Angata, T. et al. (2002) J. Biol. Chem. 277:24466.
Nicoll, G. et al. (1999) J. Biol. Chem. 274:34089.

Long Name

Sialic Acid Binding Ig-like Lectin 7

Entrez Gene IDs

27036 (Human)

Alternate Names

Adhesion inhibitory receptor molecule 1; adhesion inhibitory receptor molecule 1, siglec-7; AIRM1; AIRM-1; AIRM1QA79 membrane protein; CD328 antigen; CD328; CDw328; D-siglec; p75; p75/AIRM1; QA79; sialic acid binding Ig-like lectin 7; sialic acid binding immunoglobulin-like lectin 7; sialic acid-binding Ig-like lectin 7; Siglec7; Siglec-7

Related Research Areas

Citations for Recombinant Human Siglec-7/CD328 Fc Chimera Protein, CF

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

8 Citations: Showing 1 - 8
Filter your results:

Filter by:

Siglec-7 glyco-immune binding mAbs or NK cell engager biologics induce potent antitumor immunity against ovarian cancers
Authors: Bordoloi, D;Kulkarni, AJ;Adeniji, OS;Pampena, MB;Bhojnagarwala, PS;Zhao, S;Ionescu, C;Perales-Puchalt, A;Parzych, EM;Zhu, X;Ali, AR;Cassel, J;Zhang, R;Betts, MR;Abdel-Mohsen, M;Weiner, DB;
Science advances
Species: Human
Sample Types: Whole Cells
Applications: ICC
Sialic Acids on Tumor Cells Modulate IgA Therapy by Neutrophils via Inhibitory Receptors Siglec-7 and Siglec-9
Authors: Chan, C;Lustig, M;Jansen, JHM;Garcia Villagrasa, L;Raymakers, L;Daamen, LA;Valerius, T;van Tetering, G;Leusen, JHW;
Cancers
Species: Human
Sample Types: Whole Cells
Applications: Bioassay
Targeting stromal cell sialylation reverses T cell-mediated immunosuppression in the tumor microenvironment
Authors: Egan, H;Treacy, O;Lynch, K;Leonard, NA;O'Malley, G;Reidy, E;O'Neill, A;Corry, SM;De Veirman, K;Vanderkerken, K;Egan, LJ;Ritter, T;Hogan, AM;Redmond, K;Peng, L;Che, J;Gatlin, W;Jayaraman, P;Sheehan, M;Canney, A;Hynes, SO;Kerr, EM;Dunne, PD;O'Dwyer, ME;Ryan, AE;
Cell reports
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry
Decidual glycodelin-A polarizes human monocytes towards a decidual macrophage-like phenotype via siglec-7
Authors: M Vijayan, CL Lee, VHH Wong, X Wang, K Bai, J Wu, H Koistinen, M Seppälä, KF Lee, WSB Yeung, EHY Ng, PCN Chiu
J. Cell. Sci., 2020-07-23;0(0):.
Species: Human
Sample Types: Protein
Applications: Immunoprecipitation
Broad and direct interaction between TLR and Siglec families of pattern recognition receptors and its regulation by Neu1.
Authors: Chen, Guo-Yun, Brown, Nicholas, Wu, Wei, Khedri, Zahra, Yu, Hai, Chen, Xi, van de Vlekkert, Diantha, D'Azzo, Alessand, Zheng, Pan, Liu, Yang
Elife, 2014-09-03;3(0):e04066.
Species: Human
Sample Types: Cell Lysates
Applications: Bioassay
Glycocalyx engineering reveals a Siglec-based mechanism for NK cell immunoevasion.
Authors: Hudak, Jason E, Canham, Stephen, Bertozzi, Carolyn
Nat Chem Biol, 2013-11-24;10(1):69-75.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry
Engagement of Siglec-7 receptor induces a pro-inflammatory response selectively in monocytes.
Authors: Varchetta S, Brunetta E, Roberto A, Mikulak J, Hudspeth K, Mondelli M, Mavilio D
PLoS ONE, 2012-09-28;7(9):e45821.
Species: Bacteria - E. Coli, C. albicans
Sample Types: Whole Cells
Applications: Binding Assay
Siglecs facilitate HIV-1 infection of macrophages through adhesion with viral sialic acids.
Authors: Zou Z, Chastain A, Moir S
PLoS ONE, 2011-09-08;6(9):e24559.
Species: Virus
Sample Types: Virus
Applications: Surface Plasmon Resonance