Recombinant Human SIRP beta 2 Fc Chimera Protein, CF

Catalog #: 9998-SB Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
9998-SB-050

Recombinant Human SIRP beta 2 Fc Chimera Protein Binding Activity

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Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Human SIRP beta 2 Fc Chimera Protein, CF Summary

Product Specifications

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

Activity

Measured by its binding ability in a functional ELISA. When Recombinant Human SIRP beta 2 Fc Chimera is immobilized at 1 µg/mL (100 µL/well), Biotinylated Recombinant Human CD300B/LIMR5 Fc Chimera binds with an ED₅₀ of 2-10 μg/mL.

Source

Human embryonic kidney cell, HEK293-derived human SIRP beta 2 protein

Human SIRP beta 2 (Gln33-Gly287) Accession # Q5JXA9	IEGRMD	Human IgG₁ (Pro100-Lys330)
N-terminus		C-terminus

Accession #

Q5JXA9

N-terminal Sequence
Analysis

No results obtained. Gln33 inferred from enzymatic pyroglutamate treatment revealing Ser34.

Structure / Form

Disulfide-linked homodimer

Predicted Molecular Mass

55 kDa

SDS-PAGE

77-85 kDa, reducing conditions

Product Datasheets

9998-SB

Product Datasheet

COA

SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

9998-SB

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution	Reconstitute at 500 μg/mL in PBS.
Shipping	The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, ≤ -20 °C under sterile conditions after reconstitution.

Scientific Data

Binding Activity

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When Recombinant Human SIRP beta 2 Fc Chimera (Catalog # 9998-SB) is immobilized at 1 µg/mL, Biotinylated Recombinant CD300B/LMIR-5 Fc Chimera binds with an ED₅₀ of 2-10 μg/mL.

SDS-PAGE

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2 μg/lane of Recombinant Human SIRP beta 2 Fc Chimera was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 77-85 kDa and 150-170 kDa, respectively.

Reconstitution Calculator

Background: SIRP beta 2

Signal-regulatory protein beta-2(SIRP-beta-2), is a ~37 kDa monomeric single pass type I membrane glycoprotein. It belongs to the SIRP/SHPS (CD172) family of the immunoglobulin (Ig) superfamily (1). The SIRP family are paired receptors that have similar extracellular domains but differing C-terminal domains and functions (1). SIRP-beta-2 contains an N-terminal signal peptide (aa1-32), two extracellular Ig-like domains: a V-type 1 (aa 33-143) and a V-type 2 (aa 157-258) containing three potential N-linked glycosylation sites, a helical transmembrane domain (aa 288-308), and a cytoplasmic domain (aa 309-342) (1). A positively charged residue within the transmembrane domain, in analogy to SIRP-beta-1, is implicated to mediate interaction with the adaptor DAP12 protein, which contains immunoreceptor tyrosine-based activation motifs (ITAMs) (2). Proteins in the SIRP family are typically expressed in immune cells, especially in the myeloid lineages (3). Based on expression patterns, SIRPs are thought to have roles in immune regulation (4). SIRP family members role in innate immunity and host defense has potential significance as a therapeutic target in cancer and inflammation (5, 6). There are currently no known mouse or rat homologs for this protein.

References

van Beek, E.M. et al. (2005) J. Immunol. 175:7781.
Liu, Y. et al. (2005) Journal of Biological Chemistry. 280:36132.
Matozaki, T. et al. (2009) Trends in Cell Biology. 19:72.
Barclay A.N. et al. (2006) Nat Rev Immunol. 6:457.
Barclay A.N. et al. (2014) Annu Rev Immunol. 32:25.
Veillette A. (2018) Trends Immunol. 39:173.

Long Name

Signal-regulatory Protein beta 2

Entrez Gene IDs

284759 (Human)

Alternate Names

dJ776F14.2; Protein Tyrosine Phosphatase Non-Receptor Type Substrate 1-Like 3; Protein Tyrosine Phosphatase Non-Receptor Type Substrate Protein; Protein Tyrosine Phosphatase, Non-Receptor Type 1-Like; Protein Tyrosine Phosphatase, Non-Receptor Type Substrate 1-Like 3; PTPN1L; PTPNS1L3; Signal-Regulatory Protein Beta 2; Signal-Regulatory Protein Beta-2; SIRP beta 2; SIRP-beta-2; SIRPG