Recombinant Human VEGF 165b Protein

Name: Recombinant Human VEGF 165b Protein
Brand: R&D Systems
SKU: 3045-VE
Price: 420 USD

Catalog #: 3045-VE
6 Citations Datasheet / COA / SDS

Newer Version Available: BT-VEGF

NEW

Carrier Free

Catalog #	Availability	Size / Price	Qty
3045-VE-025/CF

With Carrier

Catalog #	Availability	Size / Price	Qty
3045-VE-025

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Product Details

Citations (6)

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Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Human VEGF 165b Protein Summary

Product Specifications

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

Activity

Measured by its binding ability in a functional ELISA. Serially diluted Recombinant Human (rh) VEGF_165b is added to a Recombinant Human VEGF R2/KDR/Flk‑1 Fc Chimera (Catalog # 357-KD) coated plate (2 μg/mL, 100 μL/well). Bound rhVEGF_165b is detected by a human VEGF_165b specific antibody (Catalog # MAB3045). The concentration of rhVEGF_165b that produces half-maximum response is approximately 0.12-0.6 nM.

Source

Spodoptera frugiperda, Sf 21 (stably transfected)-derived human VEGF protein
Ala27-Asp191

Accession #

AAL27435

N-terminal Sequence
Analysis

Ala27

Structure / Form

Disulfide-linked homodimer

Predicted Molecular Mass

19.1 kDa (monomer)

SDS-PAGE

19-24 kDa, reducing conditions

Product Datasheets

3045-VE (with carrier)

Product Datasheet

COA

SDS

3045-VE/CF (carrier free)

Product Datasheet

COA

SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

3045-VE

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Reconstitution	Reconstitute at 100 μg/mL in PBS containing at least 0.1% human or bovine serum albumin.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

3045-VE/CF

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution	Reconstitute at 100 μg/mL in PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Reconstitution Calculator

Background: VEGF

Vascular endothelial growth factor (VEGF or VEGF-A), also known as vascular permeability factor (VPF), is a potent mediator of both angiogenesis and vasculogenesis in the fetus and adult (1-3). It is a member of the PDGF family that is characterized by the presence of eight conserved cysteine residues and a cystine knot structure (4). Humans express two sets of alternately spliced isoforms of 121, 145, 165, 183, 189, and 206 amino acids (aa) in length (4). VEGF₁₆₅ appears to be the most abundant and potent of the angiogenic isoform set, followed by VEGF₁₂₁ and VEGF₁₈₉ (3, 4). The anti-angiogenic or “b” set of isoforms is differentially spliced to contain six alternate amino acids at the C-terminus, and are the more highly expressed isoforms in normal adult tissue (5). VEGF_165b, like VEGF₁₂₁ but unlike most angiogenic isoforms, does not bind heparins and is therefore diffusible (4, 6). Human VEGF_165b shares 88% aa sequence identity with corresponding regions of mouse and rat, 96% with porcine, 95% with canine, and 93% with feline, equine and bovine VEGF_165b, respectively. VEGFs bind the type I transmembrane receptor tyrosine kinases VEGF R1 (also called Flt-1) and VEGF R2 (Flk-1/KDR) on endothelial cells (4). Although VEGF affinity is highest for binding to VEGF R1, VEGF R2 appears to be the primary mediator of VEGF angiogenic activity (3, 4). The affinity of VEGF_165b for VEGF R2 is similar to that of VEGF₁₆₅, but VEGF_165b only partially activates VEGF R2 such that the kinase regulatory site Y1054 is not phosphorylated (6). VEGF_165b also does not bind neuropilin-1, suggesting that the functional difference between VEGF₁₆₅ and VEGF_165b maybe due to either the lack of neuropilin-1 co-signaling or unique downstream signaling activated by VEGF_165b (8). Since VEGF_165b may compete with angiogenic VEGFs for VEGF R2 sites, its ectopic expression in tumors has been shown to inhibit their growth (7).

References

Leung, D.W. et al. (1989) Science 246:1306.
Keck, P.J. et al. (1989) Science 246:1309.
Byrne, A.M. et al. (2005) J. Cell. Mol. Med. 9:777.
Robinson, C.J. and S.E. Stringer (2001) J. Cell. Sci. 114:853.
Nowak, D.G. et al. (2008) J. Cell Sci. 121:3487.
Kawamura, H. et al. (2008) Cancer Res. 68:4683.
Rennel, E.S. et al. (2008) Br. J. Cancer 98:1250.
Harper S.J. and Bates D.O. (2008) Nat. Rev. Cancer 8(11):880.

Long Name

Vascular Endothelial Growth Factor

Entrez Gene IDs

7422 (Human); 22339 (Mouse); 83785 (Rat); 281572 (Bovine); 403802 (Canine); 493845 (Feline); 30682 (Zebrafish)

Alternate Names

MVCD1; VAS; vascular endothelial growth factor A; Vascular permeability factor; Vasculotropin; VEGF; VEGFA; VEGF-A; VEGFMGC70609; VPF; VPFvascular endothelial growth factor

Related Research Areas

Citations for Recombinant Human VEGF 165b Protein

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

6 Citations: Showing 1 - 6
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CNTF Prevents Development of Outer Retinal Neovascularization Through Upregulation of CxCl10
Authors: F Bucher, E Aguilar, KV Marra, J Rapp, J Arnold, S Diaz-Aguil, C Lange, H Agostini, G Schlunck, A Stahl, M Friedlande
Invest. Ophthalmol. Vis. Sci., 2020-08-03;61(10):20.
Species: Human, Mouse
Sample Types: Spheroids, Whole Cells
Applications: Bioassay
VEGF165b, a splice variant of VEGF-A, promotes lung tumor progression and escape from anti-angiogenic therapies through a ?1 integrin/VEGFR autocrine loop
Authors: A Boudria, C Abou Fayca, T Jia, S Gout, M Keramidas, C Didier, N Lemaître, S Manet, JL Coll, AC Toffart, D Moro-Sibil, C Albiges-Ri, V Josserand, E Faurobert, C Brambilla, E Brambilla, S Gazzeri, B Eymin
Oncogene, 2018-09-07;0(0):.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay
Cross-activating c-Met/?1 integrin complex drives metastasis and invasive resistance in cancer
Authors: A Jahangiri, A Nguyen, A Chandra, MK Sidorov, G Yagnik, J Rick, SW Han, W Chen, PM Flanigan, D Schneidman, S Mascharak, M De Lay, B Imber, CC Park, K Matsumoto, K Lu, G Bergers, A Sali, WA Weiss, MK Aghi
Proc. Natl. Acad. Sci. U.S.A., 2017-09-26;0(0):.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay
Inhibition of Single Routes of Intracellular Signaling is Not Sufficient to Neutralize the Biphasic Disturbance of a Retinal Endothelial Cell Barrier Induced by VEGF-A165
Authors: HL Deissler, GK Lang, GE Lang
Cell. Physiol. Biochem., 2017-07-18;42(4):1493-1513.
Species: Bovine
Sample Types: Whole Cells
Applications: Bioassay
The control of alternative splicing by SRSF1 in myelinated afferents contributes to the development of neuropathic pain
Neurobiol Dis, 2016-09-09;96(0):186-200.
Species: Rat
Sample Types: In Vivo
Applications: In Vivo
Detection of VEGF-A(xxx)b isoforms in human tissues.
Authors: Bates, David O, Mavrou, Athina, Qiu, Yan, Carter, James G, Hamdollah-Zadeh, Maryam, Barratt, Shaney, Gammons, Melissa, Millar, Ann B, Salmon, Andrew H, Oltean, Sebastia, Harper, Steven J
PLoS ONE, 2013-07-31;8(7):e68399.
Applications: Western Blot