Recombinant Human VEGFR3/Flt-4 His-tag Avi-tag Protein, CF

Catalog #: AVI10384 Datasheet / COA / SDS

Biotinylated

Catalog #	Availability	Size / Price	Qty
AVI10384-050

Recombinant Human VEGFR3/Flt-4 His-tag Avi-tag Protein Binding Activity

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Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Human VEGFR3/Flt-4 His-tag Avi-tag Protein, CF Summary

Learn more about Avi-tag Biotinylated Proteins

Product Specifications

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

Activity

The biotin to protein ratio is greater than 0.7 as determined by the HABA assay. Measured by its binding ability in a functional ELISA. When Recombinant Human VEGF-C (Catalog # 9199-VC) is immobilized at 0.5 µg/mL (100 µL/well), Biotinylated Recombinant Human VEGFR3/Flt-4 His-tag Avi-tag (Catalog # AVI10384) binds with an ED₅₀ of 15-90 ng/mL.

Source

Human embryonic kidney cell, HEK293-derived human VEGFR3/Flt-4 protein

Human VEGFR3/Flt-4 (Tyr25-Glu775) Accession # P35916.3	HHHHHH	Avi-tag
N-terminus		C-terminus

Accession #

P35916.3

N-terminal Sequence
Analysis

Tyr25

Structure / Form

Monomer, biotinylated via Avi-tag

Predicted Molecular Mass

87 kDa

SDS-PAGE

115-140 kDa, 72-89 kDa and 60-70 kDa

Product Datasheets

AVI10384

Product Datasheet

COA

SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

AVI10384

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution	Reconstitute at 500 μg/mL in PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Scientific Data

Binding Activity

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When Recombinant Human VEGF-C (Catalog # 9199-VC) is immobilized at 0.5 µg/mL (100 µL/well), Biotinylated Recombinant Human VEGFR3/Flt-4 His-tag Avi-tag (Catalog # AVI10384) binds with an ED₅₀ of 15-90 ng/mL.

Reconstitution Calculator

Background: VEGFR3/Flt-4

VEGFR2 (KDR/Flk-1), VEGFR1 (Flt‑1) and VEGFR3 (Flt‑4) belong to the class III subfamily of receptor tyrosine kinases (RTKs). All three receptors contain seven immunoglobulin-like repeats in their extracellular domains and kinase insert domains in their intracellular regions. The expression of VEGFR1, 2, and 3 is almost exclusively restricted to the endothelial cells. These receptors are likely to play essential roles in vasculogenesis and angiogenesis. VEGFR3 cDNA encodes a 1298 amino acid (aa) residue precursor protein with a 24 aa residue signal peptide. Mature VEGFR3 is composed of a 751 aa residue extracellular domain, a 22 aa residue transmembrane domain and a 482 aa residue cytoplasmic domain. Both VEGF-C and VEGF-D have been shown to bind and activate VEGFR3 (Flt‑4). VEGFR3 is widely expressed in the early embryo but becomes restricted to lymphatic endothelia at later stages of development. It is likely that VEGFR3 may be important for lymph angiogenesis.

References

Ferra, N. and R. Davis-Smyth (1997) Endocrine Reviews 18:4.

Long Name

Vascular Endothelial Growth Factor Receptor 3

Entrez Gene IDs

2324 (Human); 14257 (Mouse)

Alternate Names

EC 2.7.10; EC 2.7.10.1; FLT4; Flt-4; fms-related tyrosine kinase 4; LMPH1A; PCLFLT41; soluble VEGFR3 variant 1; soluble VEGFR3 variant 2; soluble VEGFR3 variant 3; Tyrosine-protein kinase receptor FLT4; vascular endothelial growth factor receptor 3; VEGF R3; VEGFR3; VEGFR-3; VEGFR3Fms-like tyrosine kinase 4