Recombinant Human VSIG1 Fc Chimera Protein, CF

Catalog #: 9708-VS Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
9708-VS-050

Recombinant Human VSIG1 Fc Chimera Protein Bioactivity

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Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Human VSIG1 Fc Chimera Protein, CF Summary

Product Specifications

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

Activity

Measured by its ability to inhibit anti-CD3 antibody induced IL-17 secretion by human peripheral blood mononuclear cells (PBMC). The ED₅₀ for this effect is 5-25 μg/mL.

Source

Mouse myeloma cell line, NS0-derived human VSIG1 protein

Human VSIG1 (Val22-Gly234) Accession # Q86XK7-1	IEGRMD	Human IgG₁ (Pro100-Lys330)
N-terminus		C-terminus

Accession #

Q86XK7-1

N-terminal Sequence
Analysis

Val22

Structure / Form

Disulfide-linked homodimer

Predicted Molecular Mass

50 kDa

SDS-PAGE

68-84 kDa, reducing conditions

Product Datasheets

9708-VS

Product Datasheet

COA

SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

9708-VS

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution	Reconstitute at 200 μg/mL in PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Scientific Data

Bioactivity

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Recombinant Human VSIG1 Fc Chimera (Catalog # 9708‑VS) inhibits IL-17 secretion by human peripheral blood mononuclear cells (PBMC) in the presence of anti-CD3 antibody. The ED₅₀ for this effect is 5-25 μg/mL.

Reconstitution Calculator

Background: VSIG1

VSIG1 (V-set and immunoglobulin domain-containing protein 1), also known as Glycoprotein A34, is a member of the JAM (junctional adhesion molecule) family of proteins (1, 2). It is predominantly expressed in stomach and testis, and was also detected in gastric, esophageal and ovarian cancers (2). VSIG1 is required for the proper differentiation of glandular gastric epithelia, and also plays a role as tumor suppressor (3, 4). This type I transmembrane glycoprotein consists of a 21 aa signal peptide, a 211 aa extracellular domain (ECD), a 21 aa transmembrane domain and 134 aa cytoplasmic domain. Within the ECD, human VSIG1 shares 81% and 83% amino acid sequence identity with mouse and rat VSIG1, respectively. Our studies at R&D Systems show that VSIG1 inhibits T cell activation, including IL-17 and Interferon gamma production.

References

Kim, E. et al. (2010) Mol. Cells 30:443.
Scanlan, M.J. et al. (2006) Cancer Immun. 6:2.
Oidovasambuu, O. (2011) PLoS One 10:e25908.
Inoue, Y. et al. (2017) Cancer Sci. 8:1701.

Long Name

V-Set and Immunoglobulin Domain Containing 1

Entrez Gene IDs

340547 (Human); 78789 (Mouse); 315920 (Rat); 102123142 (Cynomolgus Monkey)

Alternate Names

Cell surface A33 antigen; dJ889N15.1; Glycoprotein A34; GPA34; MGC44287,1700062D20Rik; V-set and immunoglobulin domain containing 1; V-set and immunoglobulin domain-containing protein 1; VSIG1