Recombinant Mouse Cadherin-4/R-Cadherin Protein, CF
Recombinant Mouse Cadherin-4/R-Cadherin Protein, CF Summary
Product Specifications
Met1-Ala731 with a C-terminal 6-His tag
Analysis
Product Datasheets
Carrier Free
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
6677-CA
Formulation | Lyophilized from a 0.2 μm filtered solution in PBS. |
Reconstitution | Reconstitute at 400 μg/mL in PBS. |
Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
Stability & Storage: | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
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Reconstitution Calculator
Background: Cadherin-4/R-Cadherin
Cadherin‑4, also known as R‑Cadherin, is a 120‑140 kDa type I transmembrane protein belonging to the Cadherin superfamily of calcium‑dependent adhesion molecules. Cadherins are involved in multiple processes including embryonic development, cell migration, and maintenance of epithelial integrity (1, 2). Mouse Cadherin‑4 is synthesized with a 20 amino acid (aa) signal peptide and a 146 aa N‑terminal propeptide. The mature cell surface‑expressed protein consists of a 565 amino acid (aa) extracellular domain (ECD) that contains five Cadherin repeats, a 22 aa transmembrane segment, and a 160 aa cytoplasmic domain (3, 4). Within the propeptide and ECD, mouse Cadherin‑4 shares 92% and 98% aa sequence identity with human and rat Cadherin‑4, respectively. Cadherin‑4 is expressed in epithelial cells, vascular smooth muscle cells, glial and neuronal cells, pancreatic beta ‑cells, thyroid follicular cells, and bone marrow Lin‑ hematopoietic stem cells (4 ‑ 10). It interacts in cis to form homodimers as well as heterodimers with N‑Cadherin which function as adhesion multimers in trans-configuration (3, 11, 12). It is down‑regulated in invasive breast cancer but up‑regulated in rhabdomyosarcoma and has been shown to exert both positive and negative effects on cell migration and tumor cell invasiveness (5, 13, 14). Cadherin‑4 is involved in a variety of homing processes including guidance of the optic nerve and pioneer axons in early brain development, branching and guidance of the retinal vasculature, and targeting of hematopoietic stem cells to sites of ischemia (7, 10, 15, 16). Cadherin‑4 additionally binds to KLRG1, an inhibitory receptor expressed on NK cells (17).
- Pokutta, S. and W.I. Weis (2007) Annu. Rev. Cell Dev. Biol. 23:237.
- Gumbiner, B.M. (2005) Nat. Rev. Mol. Cell Biol. 6:622.
- Matsunami, H. et al. (1993) J. Cell Sci. 106:401.
- Hutton, J.C. et al. (1993) Mol. Endocrinol. 7:1151.
- Agiostratidou, G. et al. (2009) Cancer Res. 69:5030.
- Slater, S.C. et al. (2004) Arterioscler. Thromb. Vasc. Biol. 24:1204.
- Gerhardt, H. et al. (2000) Glia 31:131.
- Shibuya, Y. et al. (2005) Kobe J. Med. Sci. 51:35.
- Fagman, H. et al. (2003) Endocrinology 144:3618.
- Dorrell, M.I. et al. (2004) Blood 103:3420.
- Shan, W-S. et al. (2000) J. Cell Biol. 148:579.
- Murase, S. et al. (2000) Biochem. Biophys. Res. Commun. 276:1191.
- Kucharczak, J. et al. (2008) Cancer Res. 68:6559.
- Johnson, E. et al. (2004) J. Biol. Chem. 279:31041.
- Andrews, G.L. and G.S. Mastick (2003) J. Neurosci. 23:9873.
- Dorrell, M.I. et al. (2002) Invest. Ophthalmol. Vis. Sci. 43:3500.
- Ito, M. et al. (2006) J. Exp. Med. 203:289.
Citation for Recombinant Mouse Cadherin-4/R-Cadherin Protein, CF
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
1 Citation: Showing 1 - 1
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Secretomics reveals gelatinase substrates at the blood-brain barrier that are implicated in astroglial barrier function
Authors: Burmeister, M;Fraunenstein, A;Kahms, M;Arends, L;Gerwien, H;Deshpande, T;Kuhlmann, T;Gross, CC;Naik, VN;Wiendl, H;Klingauf, J;Meissner, F;Sorokin, L;
Science advances
Species: Mouse
Sample Types: Recombinant Protein
Applications: Bioassay
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