Recombinant Mouse CCL24/Eotaxin-2/MPIF-2 (aa 24-119) Protein

Catalog #: 528-MP Datasheet / COA / SDS

Carrier Free

Catalog #	Availability	Size / Price	Qty
528-MP-025/CF

With Carrier

Catalog #	Availability	Size / Price	Qty
528-MP-025

R&D Systems Recombinant Proteins and Enzymes

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Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Mouse CCL24/Eotaxin-2/MPIF-2 (aa 24-119) Protein Summary

Product Specifications

Purity

>95%, by SDS-PAGE under reducing conditions and visualized by silver stain

Endotoxin Level

<0.01 EU per 1 μg of the protein by the LAL method.

Activity

Measured by its ability to chemoattract BaF3 mouse pro‑B cells transfected with mouse CCR3. The ED₅₀ for this effect is 150-600 ng/mL.
In the same assay, the ED₅₀ of the aa 27-119 mouse Eotaxin-2 variant (R&D Systems, Catalog # 1017-MP) is 1-5 ng/mL. The aa 24-119 mouse Eotaxin-2 variant is about 150-fold less active than the aa 27-119 mouse Eotaxin-2 variant.

Source

E. coli-derived mouse CCL24/Eotaxin-2/MPIF-2 protein
Ile24-Val119

Accession #

Q9JKC0.1

N-terminal Sequence
Analysis

Ile24

Predicted Molecular Mass

10.6 kDa

Product Datasheets

528-MP (with carrier)

Product Datasheet

COA

SDS

528-MP/CF (carrier free)

Product Datasheet

COA

SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

528-MP

Formulation	Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA with BSA as a carrier protein.
Reconstitution	Reconstitute at 25 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

528-MP/CF

Formulation	Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA.
Reconstitution	Reconstitute at 100 μg/mL in sterile PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Reconstitution Calculator

Background: CCL24/Eotaxin-2/MPIF-2

Eotaxin-2, also named myeloid progenitor inhibitory factor (MPIF-2), is a member of the CC chemokine subfamily and is designated CCL24. Eotaxin-2 is constitutively expressed in the jejunum and spleen. It can also be induced in the lung by allergen challenge and IL-4. LPS and IL-4 also differentially regulate the expression of Eotaxin-2 in monocytes and macrophages. Mouse Eotaxin-2 cDNA encodes a 119 amino acid (aa) residue precursor protein that shares approximately 58% aa sequence identity with human Eotaxin-2. Functionally, Eotaxin-2 is most closely related to eotaxin/CCL11 and Eotaxin-3/CCL26. The three proteins share low sequence homology but have been shown to be potent eosinophil chemoattractants that bind and activate the chemokine receptor CCR3, a receptor that is highly expressed in eosinophils. Eotaxin-2 also has the ability to suppress myeloid cell proliferation, a biological function not shared by eotaxin. Two amino-terminally-truncated isoforms of mouse Eotaxin-2 have been produced at R&D Systems. The Val 27 - Val 119 mouse Eotaxin-2 isoform has been shown to be 150-fold more potent than the Ile 24 - Val 119 isoform as a chemoattractant for mouse BaR/3 cells transfected with mouse CCR3 (1 - 3).

References

Zimmermann, N. et al. (2000) J. Immunol. 165:5839.
Watanabe, K. et al. (2002) J. Immunol. 168:1911.
Grzegorzewski, K.J. et al. (2001) Cytokine 13:209.

Entrez Gene IDs

6369 (Human); 56221 (Mouse)

Alternate Names

CCL24; chemokine (C-C motif) ligand 24; Ckb-6; Eosinophil chemotactic protein 2; Eotaxin-2; member 24; MPIF2; MPIF-2; MPIF2Myeloid progenitor inhibitory factor 2; MPIF-2Small-inducible cytokine A24; SCYA24CK-beta-6