Recombinant Mouse CD300c2 Fc Chimera Protein, CF

Catalog #: 5838-LM Datasheet / COA / SDS

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5838-LM-050

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Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Mouse CD300c2 Fc Chimera Protein, CF Summary

Product Specifications

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

Activity

Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated human T cells. Human T lymphocytes cultured for 72 hours with PHA were incubated for an additional 3 days in 96 well plates coated with 500 ng/mL anti‑CD3 and serially diluted Recombinant Mouse CD300c2 Fc Chimera.
The ED₅₀ for this effect is 0.6‑3 µg/mL.
Optimal dilutions should be determined by each laboratory for each application.

Source

Chinese Hamster Ovary cell line, CHO-derived mouse protein

Mouse CD300c2 (Cys22-Ser193) Accession # BAC80269	IEGRMDP	Mouse IgG_2A (Glu98-Lys330)
N-terminus		C-terminus

Accession #

BAC80269

N-terminal Sequence
Analysis

Cys22

Structure / Form

Disulfide-linked homodimer

Predicted Molecular Mass

46 kDa (monomer)

SDS-PAGE

55-65 kDa, reducing conditions

Product Datasheets

5838-LM

Product Datasheet

COA

SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

5838-LM

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution	Reconstitute at 100 μg/mL in PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Reconstitution Calculator

Background: CD300c2

Leukocyte mono-Ig-like receptor 2 (LMIR2), also known as CMRF-35A, CD300c2, CLM4, DIgR1, and MAIR-II, is a 35 kDa type I transmembrane glycoprotein that belongs to the immunoregulatory signaling (IRS) family of immunoglobulin-like molecules (1). Mature mouse LMIR2 consists of a 165 amino acid (aa) extracellular domain (ECD) with one Ig-like domain, a 21 aa transmembrane segment, and a 20 aa cytoplasmic tail (2). An alternate splice form lacks two amino acids C-terminal to the Ig-like domain (3). Within the ECD, mouse LMIR2 shares 45% aa sequence identity with human LMIR2. The CMRF-35 antibody detects both LMIR2 and LMIR1/CMRF-35H which are 92% identical in their Ig-like domains (4-7). LMIR2 is expressed on the surface of subpopulations of bone marrow derived dendritic cells and mast cells, monocytes, peritoneal macrophages, marginal zone B cells, and T cells (2, 3, 6, 8). It contains at least one positively charged amino acid in its transmembrane segment which may mediate LMIR2 association with the adaptor signaling proteins DAP10, DAP12, and FcR gamma (3, 8). Cross-linking of LMIR2 on macrophages induces the secretion of the proinflammatory molecules TNF-alpha, IL-6, and CCL2 (3).

References