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Recombinant Mouse E-Cadherin Protein, CF

Catalog #: 8875-EC
5 Citations 1 Review Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
8875-EC-050
R&D Systems Recombinant Proteins and Enzymes
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Citations (5)
FAQs
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Reviews (1)
Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Mouse E-Cadherin Protein, CF Summary

Product Specifications

Purity
>95%, by SDS-PAGE with silver staining
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by the ability of the immobilized protein to support the adhesion of the MCF‑7 human breast cancer cells. The ED50 for this effect is 0.25-1 μg/mL.
Source
Mouse myeloma cell line, NS0-derived mouse E-Cadherin protein
Asp157-Val709, with a C-terminal 6-His tag
Accession #
P09803
N-terminal Sequence
Analysis
Asp157
Predicted Molecular Mass
62 kDa
SDS-PAGE
75-87 kDa, reducing conditions

Product Datasheets

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8875-EC

Product Datasheet
COA
SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

8875-EC

Formulation Lyophilized from a 0.2 μm filtered solution in MES, NaCl and CaCl2.
Reconstitution Reconstitute at 250 μg/mL in water.
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: E-Cadherin

E-Cadherin/Cadherin-1, also known as Uvomorulin in the mouse and rat, is a 120 kDa member of the Cadherin family of cell surface glycoproteins that mediate cell adhesion (1). Mouse E-Cadherin shares 92% and 81% amino acid sequence identity with the rat and human proteins, respectively (2). It is a single-pass transmembrane protein that mediates calcium-dependent epithelial cell adhesion. E-Cadherin has five extracellular EC domains that form homophilic cis-clusters between adjacent epithelial cells and trans-clusters within the same cell. E-Cadherin clusters are critical components of adherens junctions between epithelial cells and act in the formation and maintenance of the epithelial cell barrier (3, 4). The intracellular domain of E-Cadherin binds to the Catenin cytoskeletal complex, which includes p120 Catenin, beta-Catenin, alpha-Catenin, and Vinculin. E-Cadherin expression is critical for epithelial tissue homeostasis. Decreased E-Cadherin is associated with physiological and pathological epithelial-to-mesenchymal transition and cell migration, and E-Cadherin loss contributes to cancer metastasis (5). The extracellular E-Cadherin N-terminal domain can be cleaved by several proteases and is released as a soluble factor that enhances cancer cell motility and EGFR-dependent survival and proliferation (6).

References
  1. Gumbiner, B.M. (2005) Nat. Rev. Mol. Cell Biol. 6:622.
  2. Nagafuchi, A. et al. (1987) Nature 329:341.
  3. Guillot, C. and T. Lecuit (2013) Science 340:1185.
  4. Tian, X. et al. (2011) J. Biomed. Biotechnol. 2011:567305.
  5. Stemmler, M.P. (2008) Mol. Biosyst. 4:835.
  6. David, J.M. and A.K. Rajasekaran (2012) Cancer Res. 72:2917.
Entrez Gene IDs
999 (Human); 12550 (Mouse); 83502 (Rat)
Alternate Names
Arc-1; CAD1; cadherin 1, E-cadherin (epithelial); cadherin 1, type 1, E-cadherin (epithelial); Cadherin-1; calcium-dependent adhesion protein, epithelial; CAM 120/80; CD324 antigen; CD324; CDH1; CDHE; cell-CAM 120/80; Cell-CAM120/80; ECAD; ECadherin; E-Cadherin; Epithelial cadherin; LCAM; L-CAM; UVOE-Cadherin; Uvomorulin

Citations for Recombinant Mouse E-Cadherin Protein, CF

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

5 Citations: Showing 1 - 5
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  1. Targeting breast and pancreatic cancer metastasis using a dual-cadherin antibody
    Authors: DS Micalizzi, D Che, BT Nicholson, JF Edd, N Desai, ER Lang, M Toner, S Maheswaran, DT Ting, DA Haber
    Proceedings of the National Academy of Sciences of the United States of America, 2022-10-18;119(43):e2209563119.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot Control
  2. In vivo self-assembled siRNA as a modality for combination therapy of ulcerative colitis
    Authors: X Zhou, M Yu, L Ma, J Fu, J Guo, J Lei, Z Fu, Y Fu, Q Zhang, CY Zhang, X Chen
    Nature Communications, 2022-09-28;13(1):5700.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  3. Stem cell-derived synthetic embryos self-assemble by exploiting cadherin codes and cortical tension
    Authors: M Bao, J Cornwall-S, E Sanchez-Va, DY Chen, J De Jonghe, S Shadkhoo, F Hollfelder, M Thomson, DM Glover, M Zernicka-G
    Nature Cell Biology, 2022-09-13;24(9):1341-1349.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  4. Three-dimensional geometry controls division symmetry in stem cell colonies
    Authors: A Chaigne, MB Smith, RL Cavestany, E Hannezo, KJ Chalut, EK Paluch
    Journal of Cell Science, 2021-01-01;0(0):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  5. Novel peptides for deciphering structural and signalling functions of E-cadherin in mouse embryonic stem cells
    Authors: JM Segal, CM Ward
    Sci Rep, 2017-02-07;7(0):41827.
    Species: Mouse
    Sample Types:
    Applications: Surface Plasmon Resonance

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Recombinant Mouse E-Cadherin Protein, CF
By Do-Hyun Kim on 05/27/2022
Application: In vivo study