Recombinant Mouse Galectin-3 Protein, CF

Name: Recombinant Mouse Galectin-3 Protein, CF
Brand: R&D Systems
SKU: 9039-GA

Catalog #: 9039-GA
1 Citation Datasheet / COA / SDS

Newer Version Available: 9039-GAB

NEW

To be discontinued when inventory is sold

Discontinued Product

9039-GA has been discontinued and is replaced by 9039-GAB.

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All Galectin-3 Proteins and Enzymes

Product Details

Citations (1)

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Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Mouse Galectin-3 Protein, CF Summary

Product Specifications

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

Activity

Measured by the ability of the immobilized protein to support the adhesion of D10.G4.1 mouse helper T cells. The ED₅₀ for this effect is 1-5 μg/mL.

Source

Human embryonic kidney cell, HEK293-derived mouse Galectin-3 protein
Ala10-Ile264

Accession #

Q8C253

N-terminal Sequence
Analysis

Ala10

Predicted Molecular Mass

26 kDa

SDS-PAGE

25-35 kDa, reducing conditions

Product Datasheets

9039-GA

Product Datasheet

COA

SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

9039-GA

Formulation	Lyophilized from a 0.2 μm filtered solution in HEPES, NaCl, TCEP, PEG-8000 and Trehalose.
Reconstitution	Reconstitute at 100 μg/mL in PBS.
Shipping	The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Scientific Data

Bioactivity

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Recombinant Mouse Galectin-3 (Catalog # 9039-GA) supports the adhesion of D10.G4.1 mouse helper T cells. The ED₅₀ for this effect is 1-5 μg/mL, while competitor's protein does not have activity.

Reconstitution Calculator

Background: Galectin-3

Galectin-3, also known as Mac-2, L29, CBP35, and epsilon BP, is classified as a chimeric member of the Galectin superfamily and contains one carbohydrate recognition domain (CRD) linked to a nonlectin domain (1, 2). Mature mouse Galectin-3 shares 80% and 86% amino acid (aa) sequence identity with human and rat Galectin-3, respectively. Galectin-3 is a 26 kDa protein that can be nuclear, cytoplasmic, or secreted (3, 4). Nuclear Galectin-3 can modulate gene expression, while cytosolic Galectin-3 can inhibit apoptosis and can participate in exocytosis, Caveolin-mediated endocytosis, and macrophage-mediated clearance of apoptotic cells (5-7). Extracellular Galectin-3 has been shown to form high-order oligomers that promote the crosslinking of cell surface oligosacchraides as well as integrin-dependent cell adhesion and apoptosis (8-11). Galectin-3 contributes to the innate immune response against Candida albicans and Streptococcus pneumoniae, and it can facilitate acute inflammatory responses via neutrophil activation and opsonization, macrophage recruitment, and mast cell activation (12-14). Galectin-3 can also contribute to chronic inflammation and fibrosis (15). It is implicated in neuroinflammatory disorders of the central nervous system, cardiac fibrosis, and heart failure, as well as tumor growth, progression, and metastasis (16-18).

References

Robertson, M.W. et al. (1990) Biochemistry 29:8093.
Elola, M.T. et al. (2007) Cell. Mol. Life Sci. 64:1679.
Haudek, K.C. et al. (2010) Biochim. Biophys. Acta 1800:181.
Hughes, R.C. (1999) Biochim. Biophys. Acta 1473:172.
Krzeslak, A. and A. Lipinska (2004) Cell. Mol. Biol. Lett. 9:305.
Dumic, J. et al. (2006) Biochim. Biophys. Acta 1760:616.
Sano, H. et al. (2003) J. Clin. Invest. 112:389.
Ahmad, N. et al. (2004) J. Biol. Chem. 279:10841.
Friedrichs, J. et al. (2008) J. Biol. Chem. 283:32264.
Wen, Y. et al. (2006) J. Cell. Biochem. 98:115.
Zhuo, Y. et al. (2008) J. Biol. Chem. 283:22177.
Henderson, N.C. and T. Sethi (2009) Immunol. Rev. 230:160.
Kohatsu, L. et al. (2006) J. Immunol. 177:471814. Nieminen, J. et al. (2008) J. Immunol. 180:2466.
Henderson, N.C. et al. (2008) Am. J. Pathol. 172:288.
Newlaczyl, A.U. and L.G. Yu (2011) Cancer Lett. 313:123.
Sherwi, N. et al. (2012) Future Cardiol. 8:885.
Shin, T. (2013) Acta Histochem. 115:407.

Entrez Gene IDs

3958 (Human); 16854 (Mouse)

Alternate Names

AGE-R3; Carbohydrate-binding protein 35; CBP 35,35 kDa lectin; CBP35; GAL3; Gal-3; galactin-3; Galactose-specific lectin 3; Galactoside-binding protein; GALBPCBP35; Galectin3; Galectin-3; GALIG; IgE-binding protein; L29; L31; L-31; Laminin-binding protein; Lectin L-29; lectin, galactoside-binding, soluble, 3; LGALS2; LGALS3; Mac-2 antigen; Mac-2; MAC2GAL3

Related Research Areas

Citation for Recombinant Mouse Galectin-3 Protein, CF

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1