Recombinant Mouse Galectin-3 Protein Summary
Product Specifications
Ala2-Ile264
Analysis
Product Datasheets
Carrier Free
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
1197-GA
Formulation | Lyophilized from a 0.2 μm filtered solution in PBS, EDTA and DTT with BSA as a carrier protein. |
Reconstitution | Reconstitute at 250 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin. |
Shipping | The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below. |
Stability & Storage: | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
1197-GA/CF
Formulation | Lyophilized from a 0.2 μm filtered solution in PBS, EDTA and DTT. |
Reconstitution | Reconstitute at 250 μg/mL in sterile PBS. |
Shipping | The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below. |
Stability & Storage: | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
Scientific Data
Recombinant Mouse Galectin-3 (Catalog # 1197-GA) agglutinates human red blood cells. The ED50 for this effect is 5‑20 µg/mL.
Reconstitution Calculator
Background: Galectin-3
Galectin-3, also known as Mac-2, L29, CBP35, and epsilon BP, is classified as a chimeric member of the Galectin superfamily and contains one carbohydrate recognition domain (CRD) linked to a nonlectin domain (1, 2). Mature mouse Galectin-3 shares 80% and 86% amino acid (aa) sequence identity with human and rat Galectin-3, respectively. Galectin-3 is a 26 kDa protein that can be nuclear, cytoplasmic, or secreted (3, 4). Nuclear Galectin-3 can modulate gene expression, while cytosolic Galectin-3 can inhibit apoptosis and can participate in exocytosis, Caveolin-mediated endocytosis, and macrophage-mediated clearance of apoptotic cells (5-7). Extracellular Galectin-3 has been shown to form high-order oligomers that promote the cross-linking of cell surface oligosacchraides as well as integrin-dependent cell adhesion and apoptosis (8-11). Galectin-3 contributes to the innate immune response against Candida albicans and Streptococcus pneumoniae, and it can facilitate acute inflammatory responses via neutrophil activation and opsonization, macrophage recruitment, and mast cell activation (12-14). Galectin-3 can also contribute to chronic inflammation and fibrosis (15). It is implicated in neuroinflammatory disorders of the central nervous system, cardiac fibrosis, and heart failure, as well as tumor growth, progression, and metastasis (16-18).
- Robertson, M.W. et al. (1990) Biochemistry 29:8093.
- Elola, M.T. et al. (2007) Cell. Mol. Life Sci. 64:1679.
- Haudek, K.C. et al. (2010) Biochim. Biophys. Acta 1800:181.
- Hughes, R.C. (1999) Biochim. Biophys. Acta 1473:172.
- Krzeslak, A. and A. Lipinska (2004) Cell. Mol. Biol. Lett. 9:305.
- Dumic, J. et al. (2006) Biochim. Biophys. Acta 1760:616.
- Sano, H. et al. (2003) J. Clin. Invest. 112:389.
- Ahmad, N. et al. (2004) J. Biol. Chem. 279:10841.
- Friedrichs, J. et al. (2008) J. Biol. Chem. 283:32264.
- Wen, Y. et al. (2006) J. Cell. Biochem. 98:115.
- Zhuo, Y. et al. (2008) J. Biol. Chem. 283:22177.
- Henderson, N.C. and T. Sethi (2009) Immunol. Rev. 230:160.
- Kohatsu, L. et al. (2006) J. Immunol. 177:4718.
- Nieminen, J. et al. (2008) J. Immunol. 180:2466.
- Henderson, N.C. et al. (2008) Am. J. Pathol. 172:288.
- Newlaczyl, A.U. and L.G. Yu (2011) Cancer Lett. 313:123.
- Sherwi, N. et al. (2012) Future Cardiol. 8:885.
- Shin, T. (2013) Acta Histochem. 115:407.
Citations for Recombinant Mouse Galectin-3 Protein
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 8
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Galectin-3 activates spinal microglia to induce inflammatory nociception in wild type but not in mice modelling Alzheimer's disease
Authors: Sideris-Lampretsas, G;Oggero, S;Zeboudj, L;Silva, R;Bajpai, A;Dharmalingam, G;Collier, DA;Malcangio, M;
Nature communications
Species: Transgenic Mouse
Sample Types: In Vivo
Applications: In Vivo -
Proteolytic processing of galectin-3 by meprin metalloproteases is crucial for host-microbiome homeostasis
Authors: C Bülck, EEL Nyström, T Koudelka, M Mannbar-Fr, G Andresen, M Radhouani, F Tran, F Scharfenbe, F Schrell, F Armbrust, E Dahlke, B Zhao, A Vervaeke, F Theilig, P Rosenstiel, P Starkl, SP Rosshart, H Fickensche, A Tholey, GC Hansson, C Becker-Pau
Science Advances, 2023-03-31;9(13):eadf4055.
Species: Human
Sample Types: Protein
Applications: Bioassay -
Diurnal Photoreceptor Outer Segment Renewal in Mice Is Independent of Galectin-3
Authors: NJ Esposito, F Mazzoni, JA Vargas, SC Finnemann
Investigative Ophthalmology & Visual Science, 2021-02-01;62(2):7.
Species: Porcine
Sample Types: Whole Cells
Applications: Bioassay -
Galectin-3 accelerates M2 macrophage infiltration and angiogenesis in tumors.
Authors: Jia W, Kidoya H, Yamakawa D, Naito H, Takakura N
Am J Pathol, 2013-03-13;182(5):1821-31.
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay -
Inhibition of galectin-3 reduces atherosclerosis in apolipoprotein E-deficient mice.
Authors: Mackinnon, Alison C, Liu, Xiaojun, Hadoke, Patrick, Miller, Mark R, Newby, David E, Sethi, Tariq
Glycobiology, 2013-02-19;23(6):654-63.
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay -
Galectin-3 is a negative regulator of lipopolysaccharide-mediated inflammation.
Authors: Li Y, Komai-Koma M, Gilchrist DS, Hsu DK, Liu FT, Springall T, Xu D
J. Immunol., 2008-08-15;181(4):2781-9.
Applications: Bioassay -
Altered expression of galectin-3 induces cortical thymocyte depletion and premature exit of immature thymocytes during Trypanosoma cruzi infection.
Authors: Silva-Monteiro E, Reis Lorenzato L, Kenji Nihei O, Junqueira M, Rabinovich GA, Hsu DK, Liu FT, Savino W, Chammas R, Villa-Verde DM
Am. J. Pathol., 2007-02-01;170(2):546-56.
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay -
Presentation of galectin-1 by extracellular matrix triggers T cell death.
Authors: He J, Baum LG
J. Biol. Chem., 2003-11-14;279(6):4705-12.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay
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