Recombinant Mouse Leptin Protein, CF Summary
Product Specifications
Val22-Cys167, with an N-terminal Met
Analysis
Product Datasheets
Carrier Free
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
498-OB
Formulation | Lyophilized from a 0.2 μm filtered solution in Tris-HCl and NaCl. |
Reconstitution | Reconstitute at 1 mg/mL in sterile 20 mM Tris-HCI, pH 8.0. |
Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
Stability & Storage: | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
Reconstitution Calculator
Background: Leptin/OB
Leptin is a protein product of the mouse obese gene. Mice with mutations in the obese gene that block the synthesis of Leptin have been found to be obese and diabetic and to have reduced activity, metabolism and body temperature. cDNA clones encoding Leptin have been isolated from human, simian, mouse and rat cells. Mouse Leptin shares approximately 96% and 84% sequence identity with the rat and human protein, respectively. Mouse Leptin cDNA encodes a 167 amino acid residue protein with a 21 amino acid residue signal sequence that is cleaved to yield the 146 amino acid residue mature protein. The expression of Leptin mRNA has been shown to be restricted to adipose tissue.
A high-affinity receptor for Leptin (OB-R) with homology to gp130 and the G-CSF receptor has been cloned. OB-R mRNA has been shown to be expressed in the choroid plexus and in the hypothalamus. OB-R has also been identified as an isoform of B219, a sequence that is expressed in at least four isoforms in very primitive hematopoietic cell populations and in a variety of lymphohematopoietic cell lines (1-3). The possible roles of Leptin in body weight regulation, hematopoiesis and reproduction are being investigated.
- Considine, R. and J. Caro (1996) Clinical Chemistry 42:843.
- Tartaglia, L.A. et al. (1995) Cell 83:1263.
- Cioffi, J.A. et al. (1996) Nature Medicine 2:585.
Citations for Recombinant Mouse Leptin Protein, CF
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 10
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Central activation of catecholamine-independent lipolysis drives the end-stage catabolism of all adipose tissues
Authors: Zhang, X;Majumdar, A;Kim, C;Kleiboeker, B;Magee, KL;Learman, BS;Thomas, SA;Lodhi, IJ;MacDougald, OA;Scheller, EL;
bioRxiv : the preprint server for biology
Species: Transgenic Mouse
Sample Types: In Vivo
Applications: In vivo assay -
AZGP1 in POMC neurons modulates energy homeostasis and metabolism through leptin-mediated STAT3 phosphorylation
Authors: Qiu, S;Wu, Q;Wang, H;Liu, D;Chen, C;Zhu, Z;Zheng, H;Yang, G;Li, L;Yang, M;
Nature communications
Species: Mouse
Sample Types: Whole Cells, In Vivo
Applications: Bioassay, In vivo assay -
SEL1L-HRD1 ER-associated degradation regulates leptin receptor maturation and signaling in POMC neurons in diet-induced obesity
Authors: Mao, H;Kim, GH;Qi, L;
Research square
Species: Mouse
Sample Types:
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Designer Fat Cells: Adipogenic Differentiation of CRISPR-Cas9 Genome-Engineered Induced Pluripotent Stem Cells
Authors: Ely, EV;Kapinski, AT;Paradi, SG;Tang, R;Guilak, F;Collins, KH;
bioRxiv : the preprint server for biology
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay -
Maternal emulsifier consumption programs offspring metabolic and neuropsychological health in mice
Authors: Milà-Guasch, M;Ramírez, S;Llana, SR;Fos-Domènech, J;Dropmann, LM;Pozo, M;Eyre, E;Gómez-Valadés, AG;Obri, A;Haddad-Tóvolli, R;Claret, M;
PLoS biology
Species: Mouse
Sample Types: In Vivo
Applications: In Vivo -
Neural stem cell self-renewal stimulation by store-operated calcium entries in adult mouse area postrema: influence of leptin
Authors: Ben Dhaou, C;Terrié, E;Déliot, N;Harnois, T;Cousin, L;Arnault, P;Constantin, B;Moyse, E;Coronas, V;
Frontiers in cellular neuroscience
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay -
Inter-organ insulin-leptin signal crosstalk from the liver enhances survival during food shortages
Authors: K Takahashi, T Yamada, S Hosaka, K Kaneko, Y Asai, Y Munakata, J Seike, T Horiuchi, S Kodama, T Izumi, S Sawada, K Hoshikawa, J Inoue, A Masamune, Y Ueno, J Imai, H Katagiri
Cell Reports, 2023-04-27;0(0):112415.
Species: Mouse
Sample Types:
Applications: In Vivo -
The mitochondrial UPR regulator ATF5 promotes intestinal barrier function via control of the satiety response
Authors: D Chamseddin, SA Mahmud, AK Westfall, TA Castoe, RE Berg, MW Pellegrino
Cell Reports, 2022-12-13;41(11):111789.
Species: Mouse
Sample Types: In Vivo
Applications: Bioassay -
Ras drives malignancy through stem cell crosstalk with the microenvironment
Authors: S Yuan, KS Stewart, Y Yang, MD Abdusselam, SM Parigi, TY Feinberg, K Tumaneng, H Yang, JM Levorse, L Polak, D Ng, E Fuchs
Nature, 2022-11-30;0(0):.
Species: Mouse
Sample Types: In Vivo
Applications: In Vivo -
Hypothalamic TTF-1 orchestrates the sensitivity of leptin
Authors: BS Park, D Kang, KK Kim, B Jeong, TH Lee, JW Park, S Kimura, JY Yeh, GS Roh, CJ Lee, S Yang, S Yang, JG Kim, BJ Lee
Molecular Metabolism, 2022-11-11;0(0):101636.
Species: Mouse
Sample Types: In Vivo
Applications: In Vivo -
Leptin enhances social motivation and reverses chronic unpredictable stress-induced social anhedonia during adolescence
Authors: Y Lei, D Wang, Y Bai, J Nougaisse, NL Weintraub, M Guo, XY Lu
Molecular Psychiatry, 2022-09-22;0(0):.
Species: Mouse
Sample Types: In Vivo
Applications: In Vivo -
Maternal and perinatal obesity induce bronchial obstruction and pulmonary hypertension via IL-6-FoxO1-axis in later life
Authors: J Selle, K Dinger, V Jentgen, D Zanetti, J Will, T Georgomano, C Vohlen, R Wilke, B Kojonazaro, O Klymenko, J Mohr, S V Koningsb, CJ Rhodes, A Ulrich, D Hirani, T Nestler, M Odenthal, E Mahabir, S Nayakanti, S Dabral, T Wunderlich, J Priest, W Seeger, J Dötsch, SS Pullamsett, MA Alejandre
Nature Communications, 2022-07-27;13(1):4352.
Species: Human, Mouse
Sample Types: Whole Cells
Applications: Bioassay -
Hypothyroidism confers tolerance to cerebral malaria
Authors: D Rodriguez-, Á Sánchez, S Pérez-Bena, C Contreras-, A Montero-Pe, M Toledo-Cas, M Gutiérrez-, R Rodrigues-, C Folgueira, AM Briones, G Sabio, I Monedero-C, I Chávez-Coi, D Castejón, E Fernández-, J Regadera, JM Bautista, A Aranda, S Alemany
Science Advances, 2022-04-06;8(14):eabj7110.
Species: Mouse
Sample Types: In Vivo
Applications: In Vivo -
Oncostatin M suppresses browning of white adipocytes via gp130-STAT3 signaling
Authors: PP van Krieke, TS Odermatt, M Borsigova, M Blüher, S Wueest, D Konrad
Molecular Metabolism, 2021-09-20;0(0):101341.
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay -
Milk Osteopontin for Gut, Immunity and Brain Development in Preterm Pigs
Authors: K Aasmul-Ols, NL Henriksen, DN Nguyen, AB Heckmann, T Thymann, PT Sangild, SB Bering
Nutrients, 2021-07-31;13(8):.
Species: Porcine
Sample Types: Plasma
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Leptin Improves Parameters of Brown Adipose Tissue Thermogenesis in Lipodystrophic Mice
Authors: A Hoffmann, T Ebert, MK Hankir, G Flehmig, N Klöting, B Jessnitzer, U Lössner, M Stumvoll, M Blüher, M Fasshauer, A Tönjes, K Miehle, S Kralisch
Nutrients, 2021-07-22;13(8):.
Species: Mouse
Sample Types: In Vivo
Applications: In Vivo -
A critical role of hepatic GABA in the metabolic dysfunction and hyperphagia of obesity
Authors: CE Geisler, S Ghimire, SM Bruggink, KE Miller, SN Weninger, JM Kronenfeld, J Yoshino, S Klein, FA Duca, BJ Renquist
Cell Reports, 2021-06-29;35(13):109301.
Species: Mouse
Sample Types: In Vivo
Applications: In Vivo -
BACE2 suppression in mice aggravates the adverse metabolic consequences of an obesogenic diet
Authors: D Díaz-Catal, G Alcarraz-V, C Castaño, S de Pablo, J Rodríguez-, A Fernández-, M Vallejo, S Ramírez, M Claret, M Parrizas, A Novials, JM Servitja
Molecular Metabolism, 2021-05-17;0(0):101251.
Species: Mouse
Sample Types: In Vivo
Applications: In Vivo -
A synergy of estradiol with leptin modulates the long non-coding RNA NEAT1/ mmu-miR-204-5p/IGF1 axis in the uterus of high-fat-diet-induced obese ovariectomized mice
Authors: A Shetty, PS Suresh
The Journal of steroid biochemistry and molecular biology, 2021-02-12;209(0):105843.
Species: Mouse
Sample Types: In Vivo
Applications: Bioassay -
Leptin regulates exon-specific transcription of the Bdnf gene via epigenetic modifications mediated by an AKT/p300 HAT cascade
Authors: C Li, F Meng, Y Lei, J Liu, J Liu, J Zhang, F Liu, C Liu, M Guo, XY Lu
Mol Psychiatry, 2020-10-26;0(0):.
Species: Mouse
Sample Types: In Vivo
Applications: Bioassay -
Effect of Lactobacillus rhamnosus GG on Energy Metabolism, Leptin Resistance, and Gut Microbiota in Mice with Diet-Induced Obesity
Authors: YC Cheng, JR Liu
Nutrients, 2020-08-24;12(9):.
Species: Mouse
Sample Types: In Vivo
Applications: Bioassay, In Vivo -
Chronic Gq signaling in AgRP neurons does not cause obesity
Authors: SN Ewbank, CA Campos, JY Chen, AJ Bowen, SL Padilla, JL Dempsey, JY Cui, RD Palmiter
Proc. Natl. Acad. Sci. U.S.A., 2020-08-06;0(0):.
Species: Mouse
Sample Types: In Vivo
Applications: Bioassay -
Inhibition of Plasminogen Activator Inhibitor-1 Activation Suppresses High Fat Diet-Induced Weight Gain via Alleviation of Hypothalamic Leptin Resistance
Authors: S Hosaka, T Yamada, K Takahashi, T Dan, K Kaneko, S Kodama, Y Asai, Y Munakata, A Endo, H Sugawara, Y Kawana, J Yamamoto, T Izumi, S Sawada, J Imai, T Miyata, H Katagiri
Front Pharmacol, 2020-06-24;11(0):943.
Species: Mouse
Sample Types: In Vivo
Applications: In Vivo -
Function of astrocyte MyD88 in high-fat-diet-induced hypothalamic inflammation
Authors: S Jin, KK Kim, BS Park, DH Kim, B Jeong, D Kang, TH Lee, JW Park, JG Kim, BJ Lee
J Neuroinflammation, 2020-06-19;17(1):195.
Species: Mouse
Sample Types: In Vivo
Applications: Bioassay -
Neuroprotective effects of leptin on cerebral ischemia through JAK2/STAT3/PGC-1-mediated mitochondrial function modulation
Authors: W Zhang, Y Jin, D Wang, J Cui
Brain Res. Bull., 2020-01-11;156(0):118-130.
Species: Mouse
Sample Types: In Vivo
Applications: In Vivo -
Exercise reduces inflammatory cell production and cardiovascular inflammation via instruction of hematopoietic progenitor cells
Authors: V Frodermann, D Rohde, G Courties, N Severe, MJ Schloss, H Amatullah, CS McAlpine, S Cremer, FF Hoyer, F Ji, ID van Koever, F Herisson, L Honold, GS Masson, S Zhang, J Grune, Y Iwamoto, SP Schmidt, GR Wojtkiewic, IH Lee, K Gustafsson, G Pasterkamp, SCA de Jager, RI Sadreyev, J MacFadyen, P Libby, P Ridker, DT Scadden, K Naxerova, KL Jeffrey, FK Swirski, M Nahrendorf
Nat. Med., 2019-11-07;25(11):1761-1771.
Species: Mouse
Sample Types: In Vivo
Applications: In Vivo -
Agrp-Specific Ablation of Scly Protects against Diet-Induced Obesity and Leptin Resistance
Authors: DJ Torres, MW Pitts, AC Hashimoto, MJ Berry
Nutrients, 2019-07-23;11(7):.
Species: Mouse
Sample Types: In Vivo
Applications: In Vivo -
Gs? deficiency in the dorsomedial hypothalamus leads to obesity, hyperphagia, and reduced thermogenesis associated with impaired leptin signaling
Authors: M Chen, EA Wilson, Z Cui, H Sun, YB Shrestha, B Podyma, CH Le, B Naglieri, K Pacak, O Gavrilova, LS Weinstein
Mol Metab, 2019-04-12;0(0):.
Species: Mouse
Sample Types: In Vivo
Applications: In Vivo -
Adipocyte OGT governs diet-induced hyperphagia and obesity
Authors: MD Li, NB Vera, Y Yang, B Zhang, W Ni, E Ziso-Qejva, S Ding, K Zhang, R Yin, S Wang, X Zhou, EX Fang, T Xu, DM Erion, X Yang
Nat Commun, 2018-11-30;9(1):5103.
Species: Mouse
Sample Types: In Vivo
Applications: In Vivo -
Paradoxical effects of obesity on T cell function during tumor progression and PD-1 checkpoint blockade
Authors: Z Wang, EG Aguilar, JI Luna, C Dunai, LT Khuat, CT Le, A Mirsoian, CM Minnar, KM Stoffel, IR Sturgill, SK Grossenbac, SS Withers, RB Rebhun, DJ Hartigan-O, G Méndez-Lag, AF Tarantal, RR Isseroff, TS Griffith, KA Schalper, A Merleev, A Saha, E Maverakis, K Kelly, R Aljumaily, S Ibrahimi, S Mukherjee, M Machiorlat, SK Vesely, DL Longo, BR Blazar, RJ Canter, WJ Murphy, AM Monjazeb
Nat. Med., 2018-11-12;0(0):.
Species: Mouse
Sample Types: In Vivo
Applications: In Vivo -
Hypothalamic Macrophage Inducible Nitric Oxide Synthase Mediates Obesity-Associated Hypothalamic Inflammation
Authors: CH Lee, HJ Kim, YS Lee, GM Kang, HS Lim, SH Lee, DK Song, O Kwon, I Hwang, M Son, K Byun, YH Sung, S Kim, JB Kim, EY Choi, YB Kim, K Kim, MN Kweon, JW Sohn, MS Kim
Cell Rep, 2018-10-23;25(4):934-946.e5.
Species: Mouse
Sample Types: In Vivo
Applications: In Vivo -
Calcium Channel CaV2.3 Subunits Regulate Hepatic Glucose Production by Modulating Leptin-Induced Excitation of Arcuate Pro-opiomelanocortin Neurons
Authors: MA Smith, L Katsouri, S Virtue, AI Choudhury, A Vidal-Puig, MLJ Ashford, DJ Withers
Cell Rep, 2018-10-09;25(2):278-287.e4.
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay -
Leptin Increases Particle-Induced Osteolysis in Female ob/ob Mice
Authors: KA Philbrick, AJ Branscum, CP Wong, RT Turner, UT Iwaniec
Sci Rep, 2018-10-04;8(1):14790.
Species: Mouse
Sample Types: In Vivo
Applications: In Vivo -
Augmented Insulin and Leptin Resistance of High Fat Diet-Fed APPswe/PS1dE9 Transgenic Mice Exacerbate Obesity and Glycemic Dysregulation
Authors: YH Lee, HC Hsu, PC Kao, YJ Shiao, SH Yeh, FS Shie, SM Hsu, CW Yeh, HK Liu, SB Yang, HJ Tsay
Int J Mol Sci, 2018-08-08;19(8):.
Species: Mouse
Sample Types: In Vivo
Applications: In Vivo -
Neuronatin regulates pancreatic ? cell insulin content and secretion
Authors: SJ Millership, G da Silva X, AI Choudhury, S Bertazzo, P Chabosseau, SM Pedroni, EE Irvine, A Montoya, P Faull, WR Taylor, J Kerr-Conte, F Pattou, J Ferrer, M Christian, RM John, M Latreille, M Liu, GA Rutter, J Scott, DJ Withers
J. Clin. Invest., 2018-07-09;0(0):.
Species: Mouse
Sample Types: In Vivo
Applications: In Vivo -
Leptin/Osteopontin Axis Regulated Type 2T Helper Cell Response in Allergic Rhinitis with Obesity
Authors: Q Zeng, X Luo, M Han, W Liu, H Li
EBioMedicine, 2018-06-07;0(0):.
Species: Mouse
Sample Types: In Vivo
Applications: In Vivo -
Time-resolved hypothalamic open flow micro-perfusion reveals normal leptin transport across the blood-brain barrier in leptin resistant mice
Authors: M Kleinert, P Kotzbeck, T Altendorfe, T Birngruber, MH Tschöp, C Clemmensen
Mol Metab, 2018-04-27;13(0):77-82.
Species: Mouse
Sample Types: In Vivo
Applications: In Vivo -
HIV-1 Uncoating and Reverse Transcription Require eEF1A Binding to Surface-Exposed Acidic Residues of the Reverse Transcriptase Thumb Domain
Authors: DJ Rawle, D Li, JE Swedberg, L Wang, DC Soares, D Harrich
MBio, 2018-03-27;9(2):.
Species: Mouse
Sample Types: Recombinant Protein
Applications: Bioassay -
Hypothalamic ER-associated degradation regulates POMC maturation, feeding, and age-associated obesity
Authors: GH Kim, G Shi, DR Somlo, L Haataja, S Song, Q Long, EA Nillni, MJ Low, P Arvan, MG Myers, L Qi
J. Clin. Invest., 2018-02-19;0(0):.
Species: Mouse
Sample Types: In Vivo
Applications: In Vivo -
Loss of pericyte smoothened activity in mice with genetic deficiency of leptin
Authors: G Xie, M Swiderska-, ML Jewell, MV Machado, GA Michelotti, RT Premont, AM Diehl
BMC Cell Biol., 2017-04-20;18(1):20.
Species: Mouse
Sample Types: In Vivo, Whole Cells
Applications: Bioassay, In Vivo -
Intranasal delivery of N-terminal modified leptin-pluronic conjugate for treatment of obesity
Authors: D Yuan, X Yi, Y Zhao, CD Poon, KM Bullock, KM Hansen, TS Salameh, SA Farr, WA Banks, AV Kabanov
J Control Release, 2017-03-24;0(0):.
Species: Mouse
Sample Types: In Vivo
Applications: In Vivo -
MC4R-dependent suppression of appetite by bone-derived lipocalin 2
Authors: I Mosialou, S Shikhel, JM Liu, A Maurizi, N Luo, Z He, Y Huang, H Zong, RA Friedman, J Barasch, P Lanzano, L Deng, RL Leibel, M Rubin, T Nicholas, W Chung, LM Zeltser, KW Williams, JE Pessin, S Kousteni
Nature, 2017-03-08;543(7645):385-390.
Species: Human
Sample Types: Whole Cells
Applications: Control -
Specific CpG hyper-methylation leads to Ankrd26 gene down-regulation in white adipose tissue of a mouse model of diet-induced obesity
Authors: GA Raciti, R Spinelli, A Desiderio, M Longo, L Parrillo, C Nigro, V D'Esposito, P Mirra, F Fiory, V Pilone, P Forestieri, P Formisano, I Pastan, C Miele, F Beguinot
Sci Rep, 2017-03-07;7(0):43526.
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay -
An ATF4/ATG5 Signaling in Hypothalamic POMC Neurons Regulates Fat Mass via Affecting Energy Expenditure
Authors: Y Xiao, Y Deng, F Yuan, T Xia, H Liu, Z Li, Z Liu, H Ying, Y Liu, Q Zhai, S Chen, F Guo
Diabetes, 2017-02-17;0(0):.
Species: Mouse
Sample Types: In Vivo
Applications: In Vivo -
Fasting selectively blocks development of acute lymphoblastic leukemia via leptin-receptor upregulation
Nat. Med, 2016-12-12;0(0):.
Species: Mouse
Sample Types: In Vivo
Applications: In Vivo -
High aminopeptidase A activity contributes to blood pressure control in ob/ob mice by AT2 receptor-dependent mechanism
Authors: Rafael Leite Morais
Am. J. Physiol. Heart Circ. Physiol, 2016-12-09;0(0):ajpheart.0048.
Species: Mouse
Sample Types: In Vivo
Applications: In Vivo -
Leptin directly promotes T-cell glycolytic metabolism to drive effector T-cell differentiation in a mouse model of autoimmunity
Eur J Immunol, 2016-06-13;0(0):.
Species: Mouse
Sample Types: In Vivo
Applications: In Vivo -
Salidroside improves glucose homeostasis in obese mice by repressing inflammation in white adipose tissues and improving leptin sensitivity in hypothalamus
Sci Rep, 2016-05-05;6(0):25399.
Species: Mouse
Sample Types: In Vivo
Applications: In Vivo -
Rapid linkage of innate immunological signals to adaptive immunity by the brain-fat axis.
Authors: Kim, Min Soo, Yan, Jingqi, Wu, Wenhe, Zhang, Guo, Zhang, Yalin, Cai, Dongshen
Nat Immunol, 2015-04-06;16(5):525-33.
Species: Mouse
Sample Types: In Vivo
Applications: In Vivo -
Leptin promotes wound healing in the skin.
Authors: Tadokoro, Susumu, Ide, Shinji, Tokuyama, Reiko, Umeki, Hirochik, Tatehara, Seiko, Kataoka, Shiki, Satomura, Kazuhito
PLoS ONE, 2015-03-23;10(3):e0121242.
Species: Mouse
Sample Types: Whole Tissue
Applications: Bioassay -
Dietary restriction protects against experimental cerebral malaria via leptin modulation and T-cell mTORC1 suppression.
Authors: Mejia P, Trevino-Villarreal J, Hine C, Harputlugil E, Lang S, Calay E, Rogers R, Wirth D, Duraisingh M, Mitchell J
Nat Commun, 2015-01-30;6(0):6050.
Species: Mouse
Sample Types: Whole Organism
Applications: In Vivo -
Three months of high-fructose feeding fails to induce excessive weight gain or leptin resistance in mice.
Authors: Tillman, Erik J, Morgan, Donald A, Rahmouni, Kamal, Swoap, Steven J
PLoS ONE, 2014-09-11;9(9):e107206.
Species: Mouse
Sample Types: In Vivo
Applications: In Vivo -
Intranasal administration as a route for drug delivery to the brain: evidence for a unique pathway for albumin.
Authors: Falcone, Joseph A, Salameh, Therese, Yi, Xiang, Cordy, Benjamin, Mortell, William, Kabanov, Alexande, Banks, William
J Pharmacol Exp Ther, 2014-07-15;351(1):54-60.
Species: Mouse
Sample Types: In Vivo
Applications: In Vivo -
Protection against high-fat diet-induced obesity in Helz2-deficient male mice due to enhanced expression of hepatic leptin receptor.
Authors: Yoshino S, Satoh T, Yamada M, Hashimoto K, Tomaru T, Katano-Toki A, Kakizaki S, Okada S, Shimizu H, Ozawa A, Tuchiya T, Ikota H, Nakazato Y, Mori M, Matozaki T, Sasaki T, Kitamura T, Mori M
Endocrinology, 2014-07-08;155(9):3459-72.
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay -
Evidence that diet-induced hyperleptinemia, but not hypothalamic gliosis, causes ghrelin resistance in NPY/AgRP neurons of male mice.
Authors: Briggs, Dana I, Lockie, Sarah H, Benzler, Jonas, Wu, Qunli, Stark, Romana, Reichenbach, Alex, Hoy, Andrew J, Lemus, Moyra B, Coleman, Harold A, Parkington, Helena C, Tups, Alex, Andrews, Zane B
Endocrinology, 2014-04-17;155(7):2411-22.
Species: Mouse
Sample Types: In Vivo
Applications: In Vivo -
Kinetics of leptin binding to the Q223R leptin receptor.
Authors: Verkerke H, Naylor C, Zabeau L, Tavernier J, Petri W, Marie C
PLoS ONE, 2014-04-17;9(4):e94843.
Species: Human
Sample Types: Antibody
Applications: Surface Plasmon Resonance -
Leptin-promoted cilia assembly is critical for normal energy balance.
Authors: Han Y, Kang G, Byun K, Ko H, Kim J, Shin M, Kim H, Gil S, Yu J, Lee B, Kim M
J Clin Invest, 2014-03-25;124(5):2193-7.
Species: Mouse
Sample Types: In Vivo
Applications: In Vivo -
Hyperleptinemia directly affects testicular maturation at different sexual stages in mice, and suppressor of cytokine signaling 3 is involved in this process.
Authors: Yuan, Miao, Huang, Guizhen, Li, Jun, Zhang, Jie, Li, Fei, Li, Kai, Gao, Bo, Zeng, Li, Shan, Wei, Lin, Ping, Huang, Lugang
Reprod Biol Endocrinol, 2014-02-06;12(0):15.
Species: Mouse
Sample Types: Whole Tissue
Applications: Bioassay -
Flurbiprofen ameliorated obesity by attenuating leptin resistance induced by endoplasmic reticulum stress.
Authors: Hosoi, Toru, Yamaguchi, Rie, Noji, Kikuko, Matsuo, Suguru, Baba, Sachiko, Toyoda, Keisuke, Suezawa, Takahiro, Kayano, Takaaki, Tanaka, Shinpei, Ozawa, Koichiro
EMBO Mol Med, 2014-01-13;6(3):335-46.
Species: Mouse
Sample Types: In Vivo
Applications: In Vivo -
Leptin deficiency impairs maturation of dendritic cells and enhances induction of regulatory T and Th17 cells.
Authors: Moraes-Vieira P, Larocca R, Bassi E, Peron J, Andrade-Oliveira V, Wasinski F, Araujo R, Thornley T, Quintana F, Basso A, Strom T, Camara N
Eur J Immunol, 2014-01-13;44(3):794-806.
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay -
Deficient leptin signaling ameliorates systemic lupus erythematosus lesions in MRL/Mp-Fas lpr mice.
Authors: Fujita Y, Fujii T, Mimori T, Sato T, Nakamura T, Iwao H, Nakajima A, Miki M, Sakai T, Kawanami T, Tanaka M, Masaki Y, Fukushima T, Okazaki T, Umehara H
J Immunol, 2014-01-03;192(3):979-84.
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay -
Thrombospondin-1 induction in the diabetic myocardium stabilizes the cardiac matrix in addition to promoting vascular rarefaction through angiopoietin-2 upregulation.
Authors: Gonzalez-Quesada C, Cavalera M, Biernacka A, Kong P, Lee D, Saxena A, Frunza O, Dobaczewski M, Shinde A, Frangogiannis N
Circ Res, 2013-09-30;113(12):1331-44.
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay -
PAQR3 has modulatory roles in obesity, energy metabolism, and leptin signaling.
Authors: Wang, Lingdi, Wang, Xiao, Li, Zhenghu, Xia, Tingting, Zhu, Lu, Liu, Bin, Zhang, Yongxian, Xiao, Fei, Pan, Yi, Liu, Yong, Guo, Feifan, Chen, Yan
Endocrinology, 2013-09-13;154(12):4525-35.
Species: Mouse
Sample Types: In Vivo
Applications: Bioassay -
BBS mutations modify phenotypic expression of CEP290-related ciliopathies.
Authors: Zhang, Yan, Seo, Seongjin, Bhattarai, Sajag, Bugge, Kevin, Searby, Charles, Zhang, Qihong, Drack, Arlene V, Stone, Edwin M, Sheffield, Val C
Hum Mol Genet, 2013-08-13;23(1):40-51.
Species: Mouse
Sample Types: In Vivo
Applications: In Vivo -
Carbon monoxide-releasing molecules reverse leptin resistance induced by endoplasmic reticulum stress.
Authors: Zheng M, Zhang Q, Joe Y, Kim S, Uddin M, Rhew H, Kim T, Ryter S, Chung H
Am J Physiol Endocrinol Metab, 2013-02-12;304(7):E780-8.
Species: Mouse
Sample Types: In Vivo
Applications: In Vivo -
Adipocyte-specific protein tyrosine phosphatase 1B deletion increases lipogenesis, adipocyte cell size and is a minor regulator of glucose homeostasis.
Authors: Owen C, Czopek A, Agouni A, Grant L, Judson R, Lees EK, Mcilroy GD, Goransson O, Welch A, Bence KK, Kahn BB, Neel BG, Mody N, Delibegovic M
PLoS ONE, 2012-02-28;7(2):e32700.
Species: Mouse
Sample Types: In Vivo
Applications: In Vivo -
Nutrient-sensing hypothalamic TXNIP links nutrient excess to energy imbalance in mice.
Authors: Blouet C, Schwartz GJ
J. Neurosci., 2011-04-20;31(16):6019-27.
Species: Mouse
Sample Types: In Vivo
Applications: In Vivo -
Intracerebroventricular Leptin Infusion Improves Glucose Homeostasis in Lean Type 2 Diabetic MKR Mice via Hepatic Vagal and Non-Vagal Mechanisms.
Authors: Li X, Wu X, Camacho R
PLoS ONE, 2011-02-17;6(2):e17058.
Species: Mouse
Sample Types: In Vivo
Applications: In Vivo -
Leptin exacerbates sepsis-mediated morbidity and mortality.
Authors: Shapiro NI, Khankin EV, van Meurs M
J. Immunol., 2010-06-02;185(1):517-24.
Species: Mouse
Sample Types: In Vivo
Applications: In Vivo -
Enhanced hypothalamic leptin signaling in mice lacking dopamine D2 receptors.
Authors: Kim KS, Yoon YR, Lee HJ, Yoon S, Kim SY, Shin SW, An JJ, Kim MS, Choi SY, Sun W, Baik JH
J. Biol. Chem., 2010-01-15;285(12):8905-17.
Species: Human, Mouse
Sample Types: In Vivo, Whole Cells
Applications: Bioassay, In Vivo -
Functional role of c-Jun-N-terminal kinase in feeding regulation.
Authors: Unger EK, Piper ML, Olofsson LE, Xu AW
Endocrinology, 2009-12-18;151(2):671-82.
Species: Mouse
Sample Types: In Vivo
Applications: In Vivo -
Enhanced production of IL-17A during zymosan-induced peritonitis in obese mice.
Authors: Pini M, Fantuzzi G
J. Leukoc. Biol., 2009-09-10;87(1):51-8.
Species: Mouse
Sample Types: In Vivo
Applications: In Vivo -
Leptin receptor is expressed in thymus medulla and leptin protects against thymic remodeling during endotoxemia-induced thymus involution.
Authors: Gruver AL, Ventevogel MS, Sempowski GD
J. Endocrinol., 2009-07-08;203(1):75-85.
Species: Mouse
Sample Types:
Applications: In Vivo -
Keratinocyte-derived chemokine in obesity: expression, regulation, and role in adipose macrophage infiltration and glucose homeostasis.
Authors: Neels JG, Badeanlou L, Hester KD, Samad F
J. Biol. Chem., 2009-06-03;284(31):20692-8.
Species: Mouse
Sample Types: In Vivo
Applications: In Vivo -
Leptin enhances CC-chemokine ligand expression in cultured murine macrophage.
Authors: Kiguchi N, Maeda T, Kobayashi Y, Fukazawa Y, Kishioka S
Biochem. Biophys. Res. Commun., 2009-05-03;384(3):311-5.
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay -
Liver-specific loss of lipolysis-stimulated lipoprotein receptor triggers systemic hyperlipidemia in mice.
Authors: Narvekar P, Berriel Diaz M, Krones-Herzig A, Hardeland U, Strzoda D, Stohr S, Frohme M, Herzig S
Diabetes, 2009-02-02;58(5):1040-9.
Species: Mouse
Sample Types: In Vivo
Applications: In Vivo -
Stearoyl-CoA desaturase 1 deficiency protects mice from immune-mediated liver injury.
Authors: Feng D, Wang Y, Mei Y, Xu Y, Xu H, Lu Y, Luo Q, Zhou S, Kong X, Xu L
Lab. Invest., 2008-11-10;89(2):222-30.
Species: Mouse
Sample Types: In Vivo
Applications: In Vivo -
Adiponectin blocks multiple signaling cascades associated with leptin-induced cell proliferation in Apc Min/+ colon epithelial cells.
Authors: Fenton JI, Birmingham JM, Hursting SD, Hord NG
Int. J. Cancer, 2008-06-01;122(11):2437-45.
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay -
Adipose tissue gene expression profiles in ob/ob mice treated with leptin.
Authors: Zhang W, Della-Fera MA, Hartzell DL, Hausman D, Baile CA
Life Sci., 2008-05-10;83(1):35-42.
Species: Mouse
Sample Types: In Vivo
Applications: In Vivo -
Hyperleptinemia without obesity in male mice lacking androgen receptor in adipose tissue.
Authors: Yu IC, Lin HY, Liu NC, Wang RS, Sparks JD, Yeh S, Chang C
Endocrinology, 2008-02-14;149(5):2361-8.
Species: Mouse
Sample Types: In Vivo
Applications: In Vivo -
FXR-deficiency confers increased susceptibility to torpor.
Authors: Cariou B, Bouchaert E, Abdelkarim M, Dumont J, Caron S, Fruchart JC, Burcelin R, Kuipers F, Staels B
FEBS Lett., 2007-10-12;581(27):5191-8.
Species: Mouse
Sample Types: In Vivo
Applications: In Vivo -
Leptin induces elongation of cardiac myocytes and causes eccentric left ventricular dilatation with compensation.
Authors: Abe Y, Ono K, Kawamura T, Wada H, Kita T, Shimatsu A, Hasegawa K
Am. J. Physiol. Heart Circ. Physiol., 2007-01-12;292(5):H2387-96.
Species: Mouse
Sample Types: In Vivo
Applications: In Vivo -
Inactivation of signal transducer and activator of transcription 3 in proopiomelanocortin (Pomc) neurons causes decreased pomc expression, mild obesity, and defects in compensatory refeeding.
Authors: Xu AW, Ste-Marie L, Kaelin CB, Barsh GS
Endocrinology, 2006-10-05;148(1):72-80.
Species: Mouse
Sample Types: In Vivo
Applications: In Vivo -
Activation of the cardiac ciliary neurotrophic factor receptor reverses left ventricular hypertrophy in leptin-deficient and leptin-resistant obesity.
Authors: Raju SV, Zheng M, Schuleri KH, Phan AC, Bedja D, Saraiva RM, Yiginer O, Vandegaer K, Gabrielson KL, O'Donnell CP, Berkowitz DE, Barouch LA, Hare JM
Proc. Natl. Acad. Sci. U.S.A., 2006-03-06;103(11):4222-7.
Species: Mouse
Sample Types: In Vivo
Applications: In Vivo -
Leptin neutralization interferes with pathogenic T cell autoreactivity in autoimmune encephalomyelitis.
Authors: De Rosa V, Procaccini C, La Cava A, Chieffi P, Nicoletti GF, Fontana S, Zappacosta S, Matarese G
J. Clin. Invest., 2006-01-12;116(2):447-55.
Species: Mouse
Sample Types: In Vivo
Applications: In Vivo -
BiaCore analysis of leptin-leptin receptor interaction: evidence for 1:1 stoichiometry.
Authors: Mistrik P, Moreau F, Allen JM
Anal. Biochem., 2004-04-15;327(2):271-7.
Species: Human, Mouse
Sample Types: Recombinant Protein
Applications: Surface Plasmon Resonance -
Role of leptin deficiency in early acute renal failure during endotoxemia in ob/ob mice.
Authors: Wang W, Poole B, Mitra A, Falk S, Fantuzzi G, Lucia S, Schrier R
J. Am. Soc. Nephrol., 2004-03-01;15(3):645-9.
Species: Mouse
Sample Types: In Vivo
Applications: In Vivo -
The increased bone mass in deltaFosB transgenic mice is independent of circulating leptin levels.
Authors: Kveiborg M, Chiusaroli R, Sims NA, Wu M, Sabatakos G, Horne WC, Baron R
Endocrinology, 2002-11-01;143(11):4304-9.
Species: Mouse
Sample Types: In Vivo
Applications: In Vivo -
Leptin accelerates autoimmune diabetes in female NOD mice.
Authors: Matarese G, Sanna V, Lechler RI, Sarvetnick N, Fontana S, Zappacosta S, La Cava A
Diabetes, 2002-05-01;51(5):1356-61.
Species: Mouse
Sample Types: In Vivo
Applications: In Vivo -
Interferon-alpha suppresses leptin levels: studies in interferon-alpha treated patients with hepatitis C virus infection and murine adipocytes.
Authors: Kaser S, Kaser A, Vogel W, Patsch JR, Tilg H
Eur. Cytokine Netw., 2002-04-01;13(2):225-9.
Applications: ELISA (Standard) -
Development of high fat diet-induced obesity and leptin resistance in C57Bl/6J mice.
Authors: Lin S, Thomas TC, Storlien LH
Int. J. Obes. Relat. Metab. Disord., 2000-05-01;24(5):639-46.
Species: Mouse
Sample Types: In Vivo
Applications: In Vivo
FAQs
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Can I reconstitute this in PBS?
We strongly recommend reconstituting this protein in 20 mM Tris-HCI, pH 8.0 as indicated on the product insert. This is the most stable buffer we have found for reconstitution and subsequent storage of this protein.
Reviews for Recombinant Mouse Leptin Protein, CF
Average Rating: 4.5 (Based on 4 Reviews)
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Reason for Rating: Administered 3 ug/g to B6 mice 2x a day for two days prior to tissue harvesting to examine protein expression. This lead to a significant decrease in body weight in leptin compared to vehicle treated mice which confirms the leptins efficiency.