Recombinant Mouse LILRB4/CD85k/ILT3 Protein, CF

Catalog #: 9095-T4 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
9095-T4-050

Recombinant Mouse LILRB4/CD85k/ILT3 Protein Bioactivity

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All LILRB4/CD85k/ILT3 Proteins and Enzymes

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Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Mouse LILRB4/CD85k/ILT3 Protein, CF Summary

Product Specifications

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

Activity

Measured by its binding ability in a functional ELISA. When Recombinant Mouse LILRB4/CD85k/ILT3 is coated onto a microplate at 2 μg/mL, Recombinant Human Angiopoietin-like Protein 7/ANGPTL7 (Catalog # 914-AN) binds with a typical ED₅₀ = 20-120 ng/mL.

Source

Mouse myeloma cell line, NS0-derived mouse LILRB4/CD85k/ILT3 protein
Gly24-Lys238, with a C-terminal 6-His tag

Accession #

Q64281

N-terminal Sequence
Analysis

Gly24 & Ser37 (minor)

Predicted Molecular Mass

25 kDa

SDS-PAGE

34-42 kDa, reducing conditions

Product Datasheets

9095-T4

Product Datasheet

COA

SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

9095-T4

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution	Reconstitute at 500 μg/mL in PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Scientific Data

Bioactivity

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When Recombinant Mouse LILRB4/CD85k/ ILT3 is coated onto a microplate at 2 µg/mL, Recombinant Human Angiopoietin-like Protein 7 (Catalog # 914-AN) binds with a typical ED₅₀ of 20-120 ng/mL.

Reconstitution Calculator

Background: LILRB4/CD85k/ILT3

LILRB4, also known as ILT3, CD85k, and LIR-5, is an approximately 60 kDa transmembrane glycoprotein that negatively regulates immune cell activation (1). Mature mouse LILRB4 consists of a 215 amino acid (aa) extracellular domain with two Ig-like domains, a 22 aa transmembrane segment, and a 75 aa cytoplasmic domain with 3 immunoreceptor tyrosine-based inhibitory motifs (ITIM) (2). Within the ECD, mouse LILRB4 shares 45% and 77% aa sequence identity with human and rat LILRB4, respectively. Alternative splicing of mouse LILRB4 generates a potentially soluble isoform that lacks the transmembrane segment (2). LILRB4 is expressed on dendritic cells (DC), monocytes, macrophages, and vascular endothelial cells (EC) (3, 6, 7). Ligation of LILRB4 triggers ITIM-mediated inhibition of cell-activating signaling, leading to enhanced immune tolerance and reduced allogeneic graft rejection (3, 5, 8, 9). Soluble LILRB4 induces the differentiation of CD8⁺ T suppressor cells (Ts) that can inhibit the effector functions of CD4⁺ Th cells and CD8⁺ CTL (5, 8, 10). In turn, CD8⁺ Ts cells induce LILRB4 up-regulation and a tolerogenic phenotype in monocytes, DC, and EC (6, 7, 9, 11, 12).

References

Vlad, G. et al. (2010) Int. Rev. Immunol. 29:119.
Castells, M.C. et al. (1994) J. Biol. Chem. 269:8393.
Cella, M. et al. (1997) J. Exp. Med. 185:1743.
Heinzmann, A. et al. (2000) Eur. J. Immunogenet. 27:121.
Suciu-Foca, N. et al. (2007) J. Immunol. 178:7432.
Gleissner, C.A. et al. (2007) Eur. J. Immunol. 37:177.
Manavalan, J.S. et al. (2004) Int. Immunol. 16:1055.
Vlad, G. and N. Suciu-Foca (2012) Exp. Mol. Pathol. 93:294.
Chang, C.C. et al. (2002) Nat. Immunol. 3:237.
Vlad, G. et al. (2006) Int. Immunopharmacol. 6:1889.
Manavalan, J.S. et al. (2003) Transpl. Immunol. 11:245.
Brenk, M. et al. (2009) J. Immunol. 183:145.

Long Name

Leukocyte Immunoglobulin-like Receptor, Subfamily B (with TM and ITIM Domains), Member 4

Entrez Gene IDs

11006 (Human); 14728 (Mouse); 107128256 (Cynomolgus Monkey)

Alternate Names

CD85 antigen-like family member K; CD85k antigen; CD85k; HM18; ILT3; ILT-3; ILT3CD85K; leukocyte immunoglobulin-like receptor, subfamily B (with TM and ITIM domains); LILRB4; LIR5; LIR5LILRB5; LIR-5subfamily B, member 4; member 4