Recombinant Mouse MDL-1/CLEC5A Protein, CF

Catalog #: 8438-CL Datasheet / COA / SDS

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8438-CL-050

R&D Systems Recombinant Proteins and Enzymes

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Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Mouse MDL-1/CLEC5A Protein, CF Summary

Product Specifications

Purity

>90%, by SDS-PAGE with silver staining

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

Activity

Measured by its binding ability in a functional ELISA. When Recombinant Mouse Galectin-9 is immobilized at 0.5 μg/mL (100 μL/well), the concentration of Recombinant Mouse MDL-1/CLEC5A that produces 50% optimal binding response is approximately 0.1-0.5 μg/mL.

Source

Mouse myeloma cell line, NS0-derived mouse MDL-1/CLEC5A protein
Tyr26-Lys190, with an N-terminal 9-His tag

Accession #

Q9R007

N-terminal Sequence
Analysis

His

Predicted Molecular Mass

20 kDa

SDS-PAGE

34-45 kDa, reducing conditions

Product Datasheets

8438-CL

Product Datasheet

COA

SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

8438-CL

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution	Reconstitute at 100 μg/mL in PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Reconstitution Calculator

Background: MDL-1/CLEC5A

MDL-1 (Myeloid DAP12-associating lectin 1), also known as CLEC5A, is an approximately 40 kDa member of the C-type lectin family (1). Mature mouse MDL-1 is a glycosylated type 2 transmembrane protein that associates into a homodimer on the cell surface (2). It consists of a 165 amino acid (aa) extracellular domain (ECD) with one C-type lectin (CTL) domain and a juxtamembrane stalk region, a 21 aa transmembrane segment, and a 4 aa cytoplasmic domain (3). Within the ECD, mouse MDL-1 shares 67% and 81% aa sequence identity with human and rat MDL-1, respectively. The transmembrane segment contains a lysine residue that mediates interactions with the signaling protein DAP12 (3). Alternative splicing generates a short isoform of mouse MDL-1 that lacks 25 aa of the stalk region (3, 4). MDL-1 is expressed on monocytes, macrophages, and neutrophils (3, 4). Its expression is up-regulated on monocytes in rheumatoid arthritis (5). MDL-1 functions as a cell attachment receptor for all four serotypes of dengue virus as well as Japanese encephalitis virus, although the short isoform binds significantly more weakly (2, 6, 7). These interactions trigger DAP12 phosphorylation, the production of multiple inflammatory cytokines, vascular leakage, and disruption of the blood-brain barrier (6-8). This recombinant protein corresponds to the long isoform of mouse MDL-1.

References

Hoving, J.C. et al. (2014) Cell. Microbiol. 16:185.
Watson, A.A. et al. (2011) J. Biol. Chem. 286:24208.
Bakker, A.B.H. et al. (1999) Proc. Natl. Acad. Sci. USA 96:9792.
Aoki, N. et al. (2009) J. Leukoc. Biol. 85:508.
Chen, D.-Y. et al. (2014) PLoS ONE 9:e86105.
Chen, S.-T. et al. (2012) PLoS Pathogens 8:e1002655.
Chen, S.-T. et al. (2008) Nature 453:672.
Wu, M.-F. et al. (2013) Blood 121:95.

Long Name

Myeloid DAP12-associating Lectin

Entrez Gene IDs

23601 (Human); 23845 (Mouse)

Alternate Names

CLEC5A; CLECSF5; CLECSF5carbohydrate-recognition domain) lectin, superfamilymember 5; C-type lectin domain family 5, member A; C-type lectin superfamily member 5; MDL1; MDL-1; MDL-1MGC138304; Myeloid DAP12-associating lectin 1; myeloid DAP12-associating lectin-1