Recombinant Mouse PDGFRL Fc Chimera Protein, CF

Catalog #: 9739-PR Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
9739-PR-050

Recombinant Mouse PDGFRL Fc Chimera Protein Bioactivity

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Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Mouse PDGFRL Fc Chimera Protein, CF Summary

Product Specifications

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

Activity

Measured by its ability to inhibit proliferation of HCT-116 human colorectal carcinoma cells. The ED₅₀ for this effect is 1-6 μg/mL.

Source

Human embryonic kidney cell, HEK293-derived mouse PDGFRL protein

Mouse PDGFRL (Gln22-Ser375) Accession # Q6PE55	IEGRMDP	Mouse IgG_2a (Glu98-Lys330)
N-terminus		C-terminus

Accession #

Q6PE55

N-terminal Sequence
Analysis

No results obtained. Gln22 inferred from enzymatic pyroglutamate treatment revealing His23

Structure / Form

Disulfide-linked homodimer

Predicted Molecular Mass

67 kDa

SDS-PAGE

65-82 kDa, reducing conditions

Product Datasheets

9739-PR

Product Datasheet

COA

SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

9739-PR

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution	Reconstitute at 500 μg/mL in PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Scientific Data

Bioactivity

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Recombinant Mouse PDGFRL Fc Chimera (Catalog # 9739‑PR) inhibits HCT‑116 human Colorectal carcinoma proliferation. The ED₅₀ for this effect is 1‑6 μg/mL.

Reconstitution Calculator

Background: PDGFRL

Platelet-derived growth factor receptor-like protein (PDGFRL) is a 67 kDa glycoprotein protein consisting of two Ig-like C2-type domains. By sequence similarity, mouse PDGFRL is 90% similar to the human version and 95% similar to that of the rat. It is a secreted protein related to the class III subfamily of receptor tyrosine kinases (RTK), the platelet-derived growth factor receptors (1-5). The high frequency of loss of heterozygosity (LOH) near the gene of PDGFRL indicated in several studies that PDGFRL can be a tumor suppressor gene (TSG) in breast cancer (6-9), colorectal cancer, prostate cancer (10), non-small cell lung cancers (11) and hepatocellular carcinoma (12). A variant of PDGFRL is found to play a role in the development of Behçet disease, a complex immunoregulatory disease (13). The autoimmune role of PDGFRL is also supported by its up-regulation in a mouse model for Rheumatoid Arthritis (14). Another study also showed that PDGFRL may play a role in chondrocyte proliferation and differentiation (15).

References

Guo, F-J. et al. (2010). World J Gastroenterol. 16:1465.
Andrae, J. et al. (2008) Genes Dev. 22:1276.
Heldin, C-H. and B. Westermark (1999) Physiol. Rev. 79:1283.
Claesson-Welsh, L. et al. (1989) Proc. Natl. Acad. Sci. USA 86:4917.
Matsui, T. et al. (1989) Science 243:800.
Seitz, S. et al. (2006). Genes Chromosomes Cancer 45:612.
Yaremko, M.L. et al. (1996). Genes, Chromosome Cancer 16:189.
Seitz, S. et al. (2000). Eur J Cancer. 36:1507.
Rennstam, K. et al. (2003). Cancer Res. 63:8861.
Komiya, A. et al. (1997). Jpn J Cancer Res. 88:389.
Lerebours, F. et al. (1999). Int J Cancer. 81:854.
Kahng, Y.S. et al. (2003) J Gastroenterol Hepatol. 18:430.
Hou, S. et al. (2013). Hum Mutat. 34:74.
Fujikado N. et al. (2006). Arthritis Res.& Ther. 8:R100.
Kawata, K. et al. (2017) J Cell Biochemistry. 118:4033.

Long Name

Platelet-derived Growth Factor Receptor-like

Entrez Gene IDs

5157 (Human); 68797 (Mouse); 290771 (Rat)

Alternate Names

PDGFRL; PDGFR-Like Protein; PDGRL; platelet-derived growth factor receptor-like protein; platelet-derived growth factor receptor-like; platelet-derived growth factor-beta-like tumor suppressor; PRLTS; PRLTSPDGF receptor beta-like tumor suppressor