Recombinant Mouse SEZ6L Protein, CF

Catalog #: 4804-S6 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
4804-S6-050

R&D Systems Recombinant Proteins and Enzymes

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Recombinant Mouse SEZ6L Protein, CF Summary

Product Specifications

Purity

>95%, by SDS-PAGE with silver staining

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

Activity

Measured by its ability to induce cell clumping and inhibit neurite outgrowth of Neuro‑2A mouse neuroblastoma cells. 2 μg/mL of Recombinant Mouse SEZ6L is able to signifcantly induce cell clumping and inhibit neurite outgrowth.

Source

Mouse myeloma cell line, NS0-derived mouse SEZ6L protein
Glu32-Ser889, with a C-terminal 6-His tag

Accession #

NP_064366

N-terminal Sequence
Analysis

Glu32

Predicted Molecular Mass

94 kDa

SDS-PAGE

115-185 kDa, reducing conditions

Product Datasheets

4804-S6

Product Datasheet

COA

SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

4804-S6

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution	Reconstitute at 250 μg/mL in sterile PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Reconstitution Calculator

Background: SEZ6L

Seizure 6-like Protein (SEZ6L), also known as Brain-specific Receptor-like Protein B (BSRP-B), is a member of the SEZ6 family of proteins with a molecular weight of approximately 200 kDa (1). Mouse SEZ6L is a type I transmembrane glycoprotein composed of an extracellular domain (ECD) that contains three CUB domains and five short consensus repeats (SCRs) (1). Alternative splicing generates an isoform that contains a phenylalanine substitution for amino acids (aa) 807-872 of the fifth SCR domain (1). Mouse SEZ6L shares 80% and 97% aa sequence identity with the human and rat orthologs, respectively. It is expressed in the plasma membrane and endoplasmic reticulum of neurons and lung bronchiolar epithelium and likely regulates PKC alpha activation (1, 2). In mouse pancreatic beta cells, SEZ6L has been shown to be a substrate for BACE-2, which cleaves its ECD at a site 16 aa upstream from its transmembrane domain (3). Additionally, human SEZ6L has been shown to be involved in the maintenance of cellular cholesterol homeostasis (4). BSRP-C also known as SEZ6, has been reported to inhibit neurite outgrowth in mice (5). R&D Systems in-house testing shows that mouse SEZ6L, also exhibits this function. Genetic variations in human Sez6l have been associated with bipolar disorder I and an increased risk for lung cancer (2, 6, 7).

References