Recombinant Mouse SPARC-like 1/SPARCL1 Protein, CF

Name: Recombinant Mouse SPARC-like 1/SPARCL1 Protein, CF
Brand: R&D Systems
SKU: 4547-SL
Rating: 5 (1 reviews)

Catalog #: 4547-SL
1 Citation 1 Review Datasheet / COA / SDS

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4547-SL-050

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Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Mouse SPARC-like 1/SPARCL1 Protein, CF Summary

Product Specifications

Purity

>90%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

Activity

Measured by its ability to inhibit the cell growth of Mv1Lu mink lung epithelial cells. Schiemann, B.J. et al. (2003) Mol. Biol. Cell. 14:3977. The ED₅₀ for this effect is 0.5-2.0 µg/mL.

Source

Mouse myeloma cell line, NS0-derived mouse SPARC-like 1/SPARCL1 protein
Ile17-Phe650, with a C-terminal 10-His tag

Accession #

P70663

N-terminal Sequence
Analysis

Ile17

Predicted Molecular Mass

72.1 kDa

SDS-PAGE

110-140 kDa, reducing conditions

Product Datasheets

4547-SL

Product Datasheet

COA

SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

4547-SL

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution	Reconstitute at 100 μg/mL in sterile PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Reconstitution Calculator

Background: SPARC-like 1/SPARCL1

SPARCL1 (Secreted Protein, Acidic and Rich in Cysteines-like 1), also known as hevin, SC1 or MAST9, is a member of the SPARC family of extracellular glycoproteins (1, 2). SPARCL1 is an anti-adhesive protein that is widely expressed in tissues such as brain, heart, lung, muscle and kidney, but not liver (3, 4). Mouse SPARCL1 contains a 16 amino acid (aa) signal sequence and a 634 aa mature region that contains four domains: a 403 aa N-terminal acidic region, a 23 aa
follistatin-like domain, a 55 aa kazal-like segment and a 148 aa calcium binding domain that contains two EF hand motifs (3, 4). Mouse mature SPARCL1 shares 89%, 67%, 63%, 61%, 60% and 58% aa identity with rat, human, equine, canine, porcine and bovine SPARCL1, respectively. The follistatin-like, kazal-like and
calcium-binding domains of SPARCL1 show 61% aa identity with corresponding regions of SPARC. SPARCL1 is predicted at 75 kDa, but migrates at ~130 kDa, which has been explained either by disulfide-linked homodimerization or by glycosylation and high acidity (3 - 5). Some truncated forms have been reported. In mouse, a 55 kDa C-terminal fragment is the only form in kidney and represent a portion of SPARCL1 in other tissues (6). In humans, a 25 kDa form is increased in liver tumors that are encapsulated, while the full-length form is down-regulated in many epithelial cell-derived tumors (7, 8). SPARCL1 inhibits adhesion and spreading on a variety of substrates (5, 9). It is thought to cause antiadhesive signaling that terminates neuronal migration, consistent with production by glial and neuronal cells during development or in response to trauma (10). In tonsillar high endothelial venules (HEV), SPARCL1 may induce endothelial cell dissociation, promoting extravasation (3). SPARCL1 binds collagen; in mice, deletion causes dermal collagen fibrils that are smaller in diameter and deficient in decorin (6, 11).

References

Framson, P. E. and E. H. Sage (2004) J. Cell. Biochem. 92:679.
Sullivan, M. M. and E. H. Sage (2004) Int. J. Biochem. Cell Biol. 36:991.
Girard, J. P. and T. A. Springer (1995) Immunity 2:113.
Bendik, I. et al. (1998) Cancer Res. 58:626.
Brekken, R. A. et al. (2004) J. Histochem. Cytochem. 52:735.
Hambrock, H. O. et al. (2003) J. Biol. Chem. 278:11351.
Lau, C. P. et al. (2006) J. Pathol. 210:469.
Isler, S. G. et al. (2001) Int. J. Oncol. 18:521.
Girard, J. P. and T. A. Springer (1996) J. Biol. Chem. 271:4511.
Gongidi, V. et al. (2004) Neuron 41:57.
Sullivan, M. M. et al. (2006) J. Biol. Chem. 281:27621.

Entrez Gene IDs

8404 (Human); 13602 (Mouse)

Alternate Names

Hevin; High endothelial venule protein; MAST 9; MAST9; PIG33; proliferation-inducing protein 33; SC1; SPARC like 1; SPARCL1; SPARC-like 1 (hevin); SPARC-like 1 (mast9, hevin); SPARC-like 1; SPARC-like protein 1

Related Research Areas

Citation for Recombinant Mouse SPARC-like 1/SPARCL1 Protein, CF

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

Hevin/Sparcl1 drives pathological pain through spinal cord astrocyte and NMDA receptor signaling
Authors: G Chen, J Xu, H Luo, X Luo, SK Singh, JJ Ramirez, ML James, JP Mathew, M Berger, C Eroglu, RR Ji
JCI Insight, 2022-12-08;7(23):.
Species: Mouse
Sample Types: In Vivo
Applications: In Vivo