Recombinant Porcine IL-1 beta/IL-1F2 Protein Summary
Product Specifications
Ala115-Pro267
Analysis
Product Datasheets
Carrier Free
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
681-PI
| Formulation | Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein. |
| Reconstitution | Reconstitute at 100 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin. |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage: |
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681-PI/CF
| Formulation | Lyophilized from a 0.2 μm filtered solution in PBS. |
| Reconstitution | Reconstitute at 100 μg/mL in sterile PBS. |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage: |
|
Reconstitution Calculator
Background: IL-1 beta/IL-1F2
IL-1 is a name that designates two pleiotropic cytokines, IL-1 alpha (IL-1F1) and IL-1 beta (IL-1F2), which are the products of distinct genes. IL-1 alpha and IL-1 beta are structurally related polypeptides that share approximately 27% amino acid (aa) identity in porcine. Both proteins are produced by a wide variety of cells in response to inflammatory agents, infections, or microbial endotoxins. While IL-1 alpha and IL-1 beta are regulated independently, they bind to the same receptor and exert identical biological effects. IL-1 RI binds directly to IL-1 alpha or IL-1 beta and then associates with IL-1 R accessory protein (IL-1 R3/IL-1 R AcP) to form a high-affinity receptor complex that is competent for signal transduction. IL-1 RII has high affinity for IL-1 beta but functions as a decoy receptor and negative regulator of IL-1 beta activity. IL-1ra functions as a competitive antagonist by preventing IL-1 alpha and IL-1 beta from interacting with IL-1 RI (1 - 4). The porcine IL-1 beta cDNA encodes a 267 aa precursor. A 114 aa propeptide is cleaved intracellularly by the cysteine protease IL-1 beta -converting enzyme (Caspase-1/ICE) to generate the active cytokine (5, 6). The 17 kDa mature porcine IL-1 beta shares 63%-70% aa sequence identity with canine, cotton rat, equine, feline, human, mouse, rat, and rhesus IL-1 beta.
- Allan, S.M. et al. (2005) Nat. Rev. Immunol. 5:629.
- Boraschi, D. and A. Tagliabue (2006) Vitam. Horm. 74:229.
- Kornman, K.S. (2006) Am. J. Clin. Nutr. 83:475S.
- Isoda, K. and F. Ohsuzu (2006) J. Atheroscler. Thromb. 13:21.
- Huether, M. et al. (1993) Gene 129:285.
- Martinon, F. and J. Tschopp (2007) Cell Death Differ. 14:10.
Citations for Recombinant Porcine IL-1 beta/IL-1F2 Protein
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
11
Citations: Showing 1 - 10
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Streptococcus suis Induces Expression of Cyclooxygenase-2 in Porcine Lung Tissue
Authors: M Dresen, J Schenk, Y Berhanu We, D Vötsch, W Baumgärtne, P Valentin-W, A Nerlich
Microorganisms, 2021-02-12;9(2):.
Species: Porcine
Sample Types: Whole Cells
Applications: Bioassay -
Nanosome-Mediated Delivery Of Protein Kinase D Inhibitor Protects Chondrocytes From Interleukin-1beta-Induced Stress And Apoptotic Death
Authors: H Cho, FU Bhatti, KA Hasty, AK Yi
Int J Nanomedicine, 2019-11-11;14(0):8835-8846.
Species: Porcine
Sample Types: Whole Cells
Applications: Bioassay -
Development and validation of a multiplex fluorescent microsphere immunoassay assay for detection of porcine cytokines
Authors: SA Hall, SH Ison, C Owles, J Coe, DA Sandercock, AJ Zanella
MethodsX, 2019-05-17;6(0):1218-1227.
Species: Porcine
Sample Types: Tissue Homogenates
Applications: ELISA Standard -
IL-1? Damages Fibrocartilage and Upregulates MMP-13 Expression in Fibrochondrocytes in the Condyle of the Temporomandibular Joint
Authors: H Tabeian, BF Betti, C Dos Santos, TJ de Vries, F Lobbezoo, AV Ter Linde, B Zandieh-Do, MI Koenders, V Everts, AD Bakker
Int J Mol Sci, 2019-05-07;20(9):.
Species: Porcine
Sample Types: Whole Cells
Applications: Bioassay -
Swine Influenza Virus Induces RIPK1/DRP1-Mediated Interleukin-1 Beta Production.
Authors: Park H, Liu G, Liu Q, Zhou Y
Viruses, 2018-08-09;10(8):.
Species: Porcine
Sample Types: Cell Culture Supernates
Applications: ELISA Developmet -
NS1 protein of 2009 pandemic influenza A virus inhibits porcine NLRP3 inflammasome-mediated interleukin-1 beta production by suppressing ASC ubiquitination
Authors: HS Park, G Liu, SN Thulasi Ra, SL Landreth, Q Liu, Y Zhou
J. Virol., 2018-03-28;0(0):.
Species: Porcine
Sample Types: Cell Culture Supernates, Recombinant Protein
Applications: ELISA Standard, ELISA Developmet -
Curcuminoids extract, hydrolyzed collagen and green tea extract synergically inhibit inflammatory and catabolic mediator's synthesis by normal bovine and osteoarthritic human chondrocytes in monolayer.
Authors: Comblain F, Sanchez C, Lesponne I, Balligand M, Serisier S, Henrotin Y
PLoS ONE, 2015-03-23;10(3):e0121654.
Species: Bovine
Sample Types: Whole Cells
Applications: Bioassay -
IFN-alpha treatment enhances porcine Arterivirus infection of monocytes via upregulation of the porcine Arterivirus receptor sialoadhesin.
Authors: Delputte PL, Van Breedam W, Barbe F, Van Reeth K, Nauwynck HJ
J. Interferon Cytokine Res., 2007-09-01;27(9):757-66.
Species: Porcine
Sample Types: Whole Cells
Applications: Bioassay -
Synergism of TNF and IL-1 in the induction of matrix metalloproteinase-3 in trabecular meshwork.
Authors: Kelley MJ, Rose AY, Song K, Chen Y, Bradley JM, Rookhuizen D, Acott TS
Invest. Ophthalmol. Vis. Sci., 2007-06-01;48(6):2634-43.
Species: Porcine
Sample Types: Whole Cells
Applications: Bioassay -
Simultaneous detection of porcine proinflammatory cytokines using multiplex flow cytometry by the xMAP technology.
Authors: Johannisson A, Jonasson R, Dernfalk J, Jensen-Waern M
Cytometry A, 2006-05-01;69(5):391-5.
Applications: ELISA (Standard) -
Detection of changes in articular cartilage proteoglycan by T(1rho) magnetic resonance imaging.
Authors: Wheaton AJ, Dodge GR, Borthakur A, Kneeland JB, Schumacher HR, Reddy R
J. Orthop. Res., 2005-01-01;23(1):102-8.
Species: Porcine
Sample Types: In Vivo
Applications: In Vivo
FAQs
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Does IL-1 beta induce NO in smooth muscle?
Yes it does, based on the literature.
Reviews for Recombinant Porcine IL-1 beta/IL-1F2 Protein
Average Rating: 5 (Based on 2 Reviews)
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Recombinant Porcine IL-1b (681-PI) was used as the immunoassay standard in a sandwich ELISA using the R&D antibodies MAB6811 as capture antibody and AF681 as the detection antibody.
