Does IL-1 beta induce NO in smooth muscle?

Name: Recombinant Rabbit IL-1 beta/IL-1F2 Protein
Brand: R&D Systems
SKU: 7464-RB
Price: 365 USD

Yes it does, based on the literature.

Recombinant Rabbit IL-1 beta/IL-1F2 Protein

Catalog #: 7464-RB
1 Citation Datasheet / COA / SDS

Carrier Free

Catalog #	Availability	Size / Price	Qty
7464-RB-010/CF

With Carrier

Catalog #	Availability	Size / Price	Qty
7464-RB-010

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Product Details

Citations (1)

FAQs

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Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Rabbit IL-1 beta/IL-1F2 Protein Summary

Product Specifications

Purity

>95%, by SDS-PAGE under reducing conditions and visualized by silver stain

Endotoxin Level

<0.1 EU per 1 μg of the protein by the LAL method.

Activity

Measured in a cell proliferation assay using D10.G4.1 mouse helper T cells. Symons, J.A. et al. (1987) in Lymphokines and Interferons, a Practical Approach. Clemens, M.J. et al. (eds): IRL Press. 272. The ED₅₀ for this effect is 3-18 pg/mL.

Source

E. coli-derived rabbit IL-1 beta/IL-1F2 protein
Ala117-Ser268

Accession #

NP_001075670

N-terminal Sequence
Analysis

Ala117

Predicted Molecular Mass

17.5 kDa

SDS-PAGE

19 kDa, reducing conditions

Product Datasheets

7464-RB (with carrier)

Product Datasheet

COA

SDS

7464-RB/CF (carrier free)

Product Datasheet

COA

SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

7464-RB

Formulation	Lyophilized from a 0.2 μm filtered solution in MES, NaCl, DTT and EDTA with BSA as a carrier protein.
Reconstitution	Reconstitute at 100 μg/mL in sterile, deionized water.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

7464-RB/CF

Formulation	Lyophilized from a 0.2 μm filtered solution in MES, NaCl, DTT and EDTA.
Reconstitution	Reconstitute at 100 μg/mL in sterile, deionized water.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Reconstitution Calculator

Background: IL-1 beta/IL-1F2

IL‑1 is a name that designates two pleiotropic cytokines, IL‑1 alpha (IL‑1F1) and IL‑1 beta (IL‑1F2), which are the products of distinct genes. IL‑1 alpha and IL‑1 beta are structurally related polypeptides that share approximately 25% amino acid (aa) identity in rabbit. Both proteins are produced by a wide variety of cells in response to inflammatory agents, infections, or microbial endotoxins. While IL‑1 alpha and IL‑1 beta are regulated independently, they bind to the same receptor and exert identical biological effects. IL‑1 RI binds directly to IL‑1 alpha or IL‑1 beta and then associates with IL‑1 R accessory protein (IL‑1 R3/IL‑1 RAcP) to form a high-affinity receptor complex that is competent for signal transduction. IL‑1 RII has high affinity for IL‑1 beta but functions as a decoy receptor and negative regulator of IL‑1 beta activity. IL‑1ra functions as a competitive antagonist by preventing IL‑1 alpha and IL‑1 beta from interacting with IL‑1 RI (1‑3). The rabbit IL‑1 beta cDNA encodes a 268 aa precursor. A 116 aa propeptide is cleaved intracellularly by the cysteine protease IL‑1 beta ‑converting enzyme (Caspase‑1/ICE) to generate the active cytokine (4, 5). The 17 kDa mature rabbit IL‑1 beta shares 78%‑81% aa sequence identity with human, mouse, and rat and 64%‑72% with bovine, equine, feline, goat, human, ovine, and porcine IL‑1 beta.

References

Dinarello, C.A. (2009) Annu. Rev. Immunol. 27:519.
Boraschi, D. and A. Tagliabue (2006) Vitam. Horm. 74:229.
Weber, A. et al. (2010) Sci. Signal. 3:cm1.
Cannon, J.G. et al. (1989) J. Immunol. 142:2299.
Martinon, F. and J. Tschopp (2007) Cell Death Differ. 14:10.

Long Name

Interleukin 1 beta

Entrez Gene IDs

3553 (Human); 16176 (Mouse); 24494 (Rat); 397122 (Porcine); 403974 (Canine); 100034237 (Equine); 100135556 (Guinea Pig)

Alternate Names

catabolin; IL1 beta; IL-1 beta; IL-1; IL1B; IL-1b; IL1-BETA; IL-1F2; IL1F2IL-1 beta; interleukin 1, beta; interleukin-1 beta; preinterleukin 1 beta; pro-interleukin-1-beta

Related Research Areas

Citation for Recombinant Rabbit IL-1 beta/IL-1F2 Protein

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1