Recombinant Rat EGFR mFc Chimera Protein, CF

Catalog #: 11522-ER Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
11522-ER-050

Recombinant Rat EGFR mFc Chimera Protein Binding Activity.

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Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Rat EGFR mFc Chimera Protein, CF Summary

Product Specifications

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

Activity

Measured by its binding ability in a functional ELISA. Recombinant Rat EGFR mFc Chimera binds to Recombinant Rat EGF Protein (Catalog # 3214-EG) with an ED₅₀ of 0.250-2.50 μg/mL.

Source

Mouse myeloma cell line, NS0-derived rat EGFR protein

Rat EGFR (Leu25-Ile647) Accession # NP_113695.1	IEGRMDP	Mouse IgG_2a (Glu98-Lys330)
N-terminus		C-terminus

Accession #

NP_113695.1

N-terminal Sequence
Analysis

Leu25

Structure / Form

Disulfide-linked homodimer

Predicted Molecular Mass

97 kDa

SDS-PAGE

110-130 kDa, under reducing conditions.

Product Datasheets

11522-ER

Product Datasheet

COA

SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

11522-ER

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution	Reconstitute at 500 μg/mL in PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Scientific Data

Binding Activity

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Measured by its binding ability in a functional ELISA. Recombinant Rat EGFR mFc Chimera Protein (Catalog # 11522-ER) binds to Recombinant Rat EGF Protein (3214-EG) with an ED₅₀ of 0.250‑2.50 μg/mL.

SDS-PAGE

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2 μg/lane of Recombinant Rat EGFR mFc Chimera Protein (Catalog # 11522-ER) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 110 - 130 kDa and 220-260 kDa, respectively.

Reconstitution Calculator

Background: EGFR

Epidermal growth factor receptor (EGFR) is a member of a subfamily of receptor tyrosine kinases comprised of four members: EGFR (also known as HER-1, ErbB1, or ErbB), ErbB2 (Neu, HER-2), ErbB3 (HER-3), and ErbB4 (HER-4). All family members are type I transmembrane glycoproteins with an extracellular domain (ECD) containing two cysteine-rich domains separated by a spacer region and a cytoplasmic domain containing a tyrosine kinase domain followed by multiple tyrosine autophosphorylation sites (1, 2). Several soluble isoforms lacking the intracellular domain are generated by alternate splicing (3, 4). The mature ECD of rat EGFR shares 89% and 95% amino acid sequence identity with human and mouse EGFR, respectively. EGFR binds a subset of the EGF family ligands, including EGF, amphiregulin, TGF-alpha, betacellulin, epiregulin, HB-EGF, and epigen (1, 2). Ligand binding induces EGFR homodimerization as well as heterodimerization with ErbB2, resulting in kinase activation, heterodimerization tyrosine phosphorylation and cell signaling (5‑7). EGFR can also be recruited to form heterodimers with the ligand‑activated ErbB3 or ErbB4. EGFR signaling regulates multiple biological functions including cell proliferation, differentiation, motility, and apoptosis (5-7). EGFR is overexpressed in a wide variety of tumors and is the target of several anti-cancer drugs (8).

References

Singh, A.B. and R.C. Harris (2005) Cell. Signal. 17:1183.
Shilo, B.Z. (2005) Development 132:4017.
Guillaudeau, A. et al. (2012) PLoS One. 7:1.
Reiter J.L. et al. (2001) Genomics 71:1.
Freed, D.M. et al. (2017) Cell. 171:683.
Burgess, A.W. et al. (2003) Mol. Cell 12:541.
Faria, J.A. et al. (2016) BBRC. 478:39.
Lee, C.K. et al. (2017) J. Thoracic Oncology. 12:403.

Long Name

Epidermal Growth Factor Receptor

Entrez Gene IDs

1956 (Human); 13649 (Mouse); 24329 (Rat); 102138724 (Cynomolgus Monkey)

Alternate Names

avian erythroblastic leukemia viral (v-erb-b) oncogene homolog; cell growth inhibiting protein 40; cell proliferation-inducing protein 61; EC 2.7.10; EC 2.7.10.1; EGF R; EGFR; epidermal growth factor receptor (avian erythroblastic leukemia viral (v-erb-b)oncogene homolog); epidermal growth factor receptor; ErbB; ErbB1; ERBB1PIG61; HER1; HER-1; mENA; Proto-oncogene c-ErbB-1; Receptor tyrosine-protein kinase erbB-1