Recombinant Viral HPV E6 Protein, CF Summary
Product Specifications
Typical concentration to support E6AP-mediated p53 ubiquitination in vitro is 0.1-2 μM depending on experimental conditions.
Met1-Leu158 with a N-terminal Ser-His
Contains Cys to Ser substitutions at the following positions: 23, 58, 87, 104, 118, 147
Product Datasheets
Carrier Free
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
AP-120
| Formulation | Supplied as a solution in HEPES, NaCl, DTT. |
| Shipping | The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage: | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
Reconstitution Calculator
Background: E6
E6 (Early protein 6) is a viral protein produced in cells infected with the Human Papillomavirus. E6 forms a complex with the host cell ubiquitin ligase E6AP (E6-Associated-Protein) generating a ligase activity that polyubiquitinates tumor suppressors p53 and p73 and targets them to the 26S proteasome for degradation. As a result DNA damage and chromosomal instabilities increase, often leading to cell proliferation and cancer. The E6/E6AP complex also targets other substrates for ubiquitination, such as TERT, BAK1, FADD, and pro-CASP8—none of which appear to be substrates for E6AP in the absence of E6.
Citation for Recombinant Viral HPV E6 Protein, CF
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
1 Citation: Showing 1 - 1
-
Identifying the ubiquitination targets of E6AP by orthogonal ubiquitin transfer
Authors: Y Wang, X Liu, L Zhou, D Duong, K Bhuripanyo, B Zhao, H Zhou, R Liu, Y Bi, H Kiyokawa, J Yin
Nat Commun, 2017-12-20;8(1):2232.
Species: Yeast
Sample Types: Recombinant Protein
Applications: Bioassay
FAQs
No product specific FAQs exist for this product, however you may
View all Proteins and Enzyme FAQsReviews for Recombinant Viral HPV E6 Protein, CF
There are currently no reviews for this product. Be the first to review Recombinant Viral HPV E6 Protein, CF and earn rewards!
Have you used Recombinant Viral HPV E6 Protein, CF?
Submit a review and receive an Amazon gift card.
$25/€18/£15/$25CAN/¥75 Yuan/¥2500 Yen for a review with an image
$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image
