Simple Plex Human Cytokeratin 18 Cartridge

Catalog #: SPCKB-PS-001065 Datasheet / COA / SDS

Simple Plex Human Cytokeratin 18 assay kit for use on Ella instrument. Contains cartridge, sample diluent SD13, and wash buffer

Catalog #	Availability	Size / Price	Qty
SPCKB-PS-001065
SPCKA-PS-001065
SPCKC-PS-001065

Control Products Available

898768: Simple Plex Control for Human Cytokeratin 18

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Summary Recovery Linearity Data Examples Product Datasheets Preparation and Storage Background Related Research Areas

Simple Plex Human Cytokeratin 18 Cartridge Summary

Assay Type

Automated sandwich ELISA

Format

Microfluidic cartridge containing three Glass Nano Reactors (GNR)

Assay Length

90 minutes

Sample Type & Volume Required Per Well*

Serum (25 uL), EDTA Plasma (25 uL), Heparin Plasma (25 uL)

Sensitivity

8.52 pg/mL

Assay Range

141.0 - 86,000 pg/mL (Serum, EDTA Plasma, Heparin Plasma)

Specificity

Natural and recombinant human Cytokeratin 18.

Cross-reactivity

< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.

Interference

No significant interference observed with available related molecules.

*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay’s minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.

Product Summary

Simple Plex assay for the detection of human Cytokeratin 18 in serum and plasma (EDTA/Heparin)

Recovery

Recovery at three different spiked concentrations within the range of the assay was evaluated.

Sample Type	Average % Recovery	Range %
EDTA Plasma (n=4)	104	86-116
Heparin Plasma (n=4)	100	81-111
Serum (n=4)	105	80-115

Linearity

Samples containing and/or spiked with high concentrations of human Cytokeratin 18 were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay

Scientific Data

N/A

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Human Cytokeratin 18 Simple Plex Assay Standard Curve

Product Datasheets

Product Datasheet

COA

SDS

Preparation and Storage

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: Cytokeratin 18

Cytokeratins are cytoskeletal intermediate filament proteins that are classified as type I (acidic) and type II (neutral to basic) and associate into heterotetramers.

Entrez Gene IDs:

3875 (Human); 16668 (Mouse)

Alternate Names:

Cell proliferation-inducing gene 46 protein; cell proliferation-inducing protein 46; CK-18; CYK18; Cytokeratin 18; cytokeratin-18; K18; Keratin 18; keratin, type I cytoskeletal 18; keratin-18; KRT18