Simple Plex Human EGF Cartridge
Simple Plex Human EGF Cartridge Summary
*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay’s minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.
Product Summary
Precision
Cell Culture Supernates, Serum, Platelet-poor EDTA Plasma, Platelet-poor Heparin Plasma, Urine
Intra-Assay Precision | Inter-Assay Precision | |||
---|---|---|---|---|
Sample | 1 | 2 | 1 | 2 |
n | 16 | 16 | 9 | 9 |
Mean (pg/mL) | 30.8 | 1355 | 30.1 | 1467 |
Standard Deviation | 1.19 | 79.3 | 1.43 | 139 |
CV% | 3.9 | 5.9 | 4.8 | 9.5 |
Recovery
Recovery at three different spiked concentrations within the range of the assay was evaluated.
Sample Type | Average % Recovery | Range % |
---|---|---|
Cell Culture Media (n=2) | 114 | 112-117 |
Platelet-poor EDTA Plasma (n=3) | 92 | 74-103 |
Platelet-poor Heparin Plasma (n=3) | 89 | 70-103 |
Serum (n=5) | 93 | 83-107 |
Linearity
Scientific Data
Product Datasheets
Preparation and Storage
Background: EGF
EGF (Epidermal Growth Factor) is expressed as a transmembrane protein that is proteolytically cleaved to generate soluble forms. It signals through EGF R/ErbB1 which heterodimerizes with ErbB2, ErbB3, or ErbB4 and can also associate with PDGF R beta and HGF R/c-MET. During development, EGF regulates thymocyte differentiation, neuroglia production, and adipocyte maturation. In the adult, EGF plays a role in mammary gland lactogenesis, fibroblast mitosis, dissociation of the extracellular matrix, and cell migration.
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