Simple Plex Human Galectin-3 Cartridge

Catalog #: SPCKB-PS-000490 Datasheet / COA / SDS

Simple Plex Human Galectin-3 assay kit for use on Ella instrument. Contains cartridge, sample diluent SD13, and wash buffer

Catalog #	Availability	Size / Price	Qty
SPCKB-PS-000490
SPCKA-PS-000490
SPCKC-PS-000490

Control Products Available

898088: Simple Plex Control for Human Galectin-3

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Summary Recovery Linearity Data Examples Product Datasheets Preparation and Storage Background Related Research Areas

Simple Plex Human Galectin-3 Cartridge Summary

Assay Type

Automated sandwich ELISA

Format

Microfluidic cartridge containing three Glass Nano Reactors (GNR)

Assay Length

90 minutes

Sample Type & Volume Required Per Well*

Cell Culture Supernates (10 uL), Serum (10 uL), EDTA Plasma (10 uL), Heparin Plasma (10 uL), Cerebrospinal Fluid (25 uL)

Sensitivity

1 pg/mL

Assay Range

3.1 - 4,740 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Cerebrospinal Fluid)

Specificity

Natural and recombinant human Galectin-3.

Cross-reactivity

< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.

Interference

No significant interference observed with available related molecules.

*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay’s minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.

Product Summary

Simple Plex assay for the detection of human Galectin-3 in cell culture supernates (CCS), serum, plasma (EDTA/Heparin), and cerebrospinal fluid (CSF).

Recovery

Recovery was not necessary as Galectin-3 shows 5 points of natural linearity.

Linearity

Samples containing and/or spiked with high concentrations of human Galectin-3 were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay

Scientific Data

N/A

Human Galectin-3 Simple Plex Assay Standard Curve

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Human Galectin-3 Ella Assay Standard Curve

Product Datasheets

Product Datasheet

COA

SDS

Preparation and Storage

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: Galectin-3

The galectins constitute a large family of carbohydrate-binding proteins that function in many systems both intracellularly and following secretion. Galectins contain either one or two carbohydrate recognition domains (CRR) which mediate recognition of N-acetyl-lactosamine-containing glycoproteins. Some galectins exist in multiple isoforms due to alternative splicing. Individual galectins differ in their tissue distribution and in their carbohydrate-binding specificities.

Entrez Gene IDs:

3958 (Human); 16854 (Mouse)

Alternate Names:

AGE-R3; Carbohydrate-binding protein 35; CBP 35,35 kDa lectin; CBP35; GAL3; Gal-3; galactin-3; Galactose-specific lectin 3; Galactoside-binding protein; GALBPCBP35; Galectin3; Galectin-3; GALIG; IgE-binding protein; L29; L31; L-31; Laminin-binding protein; Lectin L-29; lectin, galactoside-binding, soluble, 3; LGALS2; LGALS3; Mac-2 antigen; Mac-2; MAC2GAL3