Simple Plex Human Total MMP-8 Cartridge

Catalog #: SPCKB-PS-009199 Datasheet / COA / SDS

Simple Plex Human Total MMP-8 assay kit for use on Ella instrument. Contains cartridge, Sample Diluent SD13, and wash buffer

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SPCKB-PS-009199
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897124: Simple Plex Control for Human Total MMP-8

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Summary Precision Recovery Linearity Data Examples Product Datasheets Preparation and Storage Background Related Research Areas

Simple Plex Human Total MMP-8 Cartridge Summary

Assay Type

Automated sandwich ELISA

Format

Microfluidic cartridge containing three Glass Nano Reactors (GNR)

Assay Length

90 minutes

Sample Type & Volume Required Per Well*

Cell Culture Supernates (10 uL), Serum (10 uL), EDTA Plasma (25 uL), Platelet-poor EDTA Plasma (25 uL), Heparin Plasma (25 uL), Platelet-poor Heparin Plasma (25 uL), Saliva (10 uL), Cerebrospinal Fluid (10 uL)

Sensitivity

1.1 pg/mL

Assay Range

5.0 - 19,007 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Platelet-poor EDTA Plasma, Heparin Plasma, Platelet-poor Heparin Plasma, Saliva, Cerebrospinal Fluid)

Specificity

Natural human active, pro, and TIMP-1 complexed MMP-8.

Cross-reactivity

< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.

Interference

No significant interference observed with available related molecules.

*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay’s minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.

Product Summary

Simple Plex assay for the detection of human Total MMP-8 (pro- and active MMP-8) in cell culture supernatant (CCS), serum, plasma (EDTA/Heparin), platelet-poor plasma (EDTA/Heparin), saliva, and cerebrospinal fluid (CSF).

Precision

Intra-Assay Precision (Precision within an assay) Each control was tested 16 times in one assay

Inter-Assay Precision (Precision between assays) Replicates of each control were tested in multiple assays performed by at least three technicians using two lots of reagents

Cell Culture Supernates, Serum, EDTA Plasma, Platelet-poor EDTA Plasma, Heparin Plasma, Platelet-poor Heparin Plasma, Saliva, Cerebrospinal Fluid

	Intra-Assay Precision		Inter-Assay Precision
Sample	1	2	1	2
n	16	16	48	48
Mean (pg/mL)	119	5958	140	6325
Standard Deviation	4.09	205	14.8	496
CV%	3.4	3.4	10.6	7.8

Recovery

Recovery at three different spiked concentrations within the range of the assay was evaluated.

Sample Type	Average % Recovery	Range %
Cell Culture Media (n=2)	100	95-106
Cerebrospinal Fluid (n=3)	94	89-97

Linearity

Samples containing and/or spiked with high concentrations of human Total MMP-8 were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay.

Scientific Data

N/A

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Human Total MMP-8 Ella Assay Standard Curve

Product Datasheets

Product Datasheet

COA

SDS

Preparation and Storage

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: MMP-8

The matrix metalloproteinases (MMPs) consist of 24 known human zinc proteases with essential roles in breaking down components of the extracellular matrix (ECM). Additional MMP substrates include cytokines, chemokines, growth factors and binding proteins, cell/cell adhesion molecules, and other proteinases. With a few exceptions, MMPs share common structural motifs including a pro-peptide domain, a catalytic domain, a hinge region, and a hemopexin-like domain. Synthesized as pro-enzymes, most MMPs are secreted before conversion to their active form. MMP activities are modulated on several levels including transcription, pro-enzyme activation, or by their endogenous inhibitors, tissue inhibitors of metalloproteinases (TIMPs). A subset of MMPs are associated with membranes and designated as membrane-type metalloproteinases (MT-MMP).

Long Name:

Matrix Metalloproteinase 8

Entrez Gene IDs:

4317 (Human); 17394 (Mouse); 63849 (Rat)

Alternate Names:

CLG1HNC; Collagenase 2; EC 3.4.24; EC 3.4.24.34; matrix metallopeptidase 8 (neutrophil collagenase); matrix metalloproteinase 8 (neutrophil collagenase); Matrix metalloproteinase-8; MMP8; MMP-8; neutrophil collagenase; PMNL collagenase; PMNL-CL