TBI
Chemical Name: (Z)-3-((1H-Benzo[d]imidazol-4-yl)methyl)-5-(3,5-difluoro-4-hydroxybenzylidene)-2-thioxoimidazolidin-4-one
Purity: ≥98%
Biological Activity
TBI is a DFHBI derivative for imaging of single Broccoli-tagged mRNA in cells. Excitation and emission maxima (λ) when bound to Broccoli are 485 and 527 nm, respectively. TBI shows higher affinity for Broccoli aptamers compared with DFHBI-1T (Cat. No. 5610) (KD values are 71 and 305 nM, respectively). Extinction coefficient = 35,100 M-1 cm-1.Optical Data for TBI
| Emission Color | Green |
|---|---|
| λabs | 485 nm |
| λem | 527 nm |
| Extinction Coefficient (ε) | 35100 M-1cm-1 |
| Quantum Yield (φ) | 0.64 |
| Application | Live-cell Imaging, Fluorescence Microscopy |
Plan Your Experiments
Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.
Spectral Viewer
Technical Data
The technical data provided above is for guidance only.
For batch specific data refer to the Certificate of Analysis.
Tocris products are intended for laboratory research use only, unless stated otherwise.
Additional Information
Product Datasheets
Reconstitution Calculator
Molarity Calculator
FAQs
No product specific FAQs exist for this product, however you may
View all Small Molecule FAQsReviews for TBI
There are currently no reviews for this product. Be the first to review TBI and earn rewards!
Have you used TBI?
Submit a review and receive an Amazon gift card.
$25/€18/£15/$25CAN/¥75 Yuan/¥2500 Yen for a review with an image
$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image
