DFHBI 1T

Biological Activity

DFHBI 1T is a mimic of green fluorescent protein (GFP) fluorophore for imaging RNA in living cells. DFHBI 1T fluorescence is activated by binding to Spinach2 or Broccoli aptamers, also binds to Squash aptamer (K_d = 45 nM). DFHBI 1T exhibits peak excitation maxima of 482 nm and peak fluorescence emission of 505 nm when bound to Spinach2, enabling imaging with GFP filter cubes. Exhibits higher specific fluorescence and lower background fluorescence when bound to Spinach2 compared with Spinach2-DFHBI.

Technical Data

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Tocris products are intended for laboratory research use only, unless stated otherwise.

Background References

1. Isothermal folding of a light-up bio-orthogonal RNA origami nanoribbon
   E Torelli, JW Kozyra, JY Gu, U Stimming, L Piantanida, K Voïtchovsk, N Krasnogor
   Sci Rep, 2018;8(1):6989.
2. In-gel imaging of RNA processing using broccoli reveals optimal aptamer expression strategies.
   Filonov et al.
   Chem.Biol., 2015;22:649
3. Plug-and-play fluorophores extend the spectral properties of Spinach.
   Song et al.
   J.Am.Chem.Soc., 2014;136:198
4. Fluorescent RNA Aptamers as a Tool to Study RNA-Modifying Enzymes.
   Svensen and Jaffrey
   Cell Chem.Biol., 2016;23:415

Product Datasheets