Human S100B Antibody

Catalog #: AF1820
6 Citations Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF1820
AF1820-SP

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Citations (6)

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human S100B Antibody Summary

Species Reactivity

Human

Specificity

Detects human S100B in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant human (rh) S100A1, rhS100A4, and rhS100A6 is observed.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

E. coli-derived recombinant human S100B
Met1-Glu92
Accession # P04271

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

0.1 µg/mL

Recombinant Human S100B

Simple Western

10 µg/mL

See below

Immunohistochemistry

5-15 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunohistochemistry

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S100B in Human Alzheimer'S Brain. S100B was detected in immersion fixed paraffin-embedded sections of human Alzheimer's brain using Goat Anti-Human S100B Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1820) at 1.7 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Simple Western

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Detection of Human S100B by Simple Western^TM. Simple Western lane view shows lysates of human brain (cerebellum) tissue, loaded at 0.2 mg/mL. A specific band was detected for S100B at approximately 4 kDa (as indicated) using 10 µg/mL of Goat Anti-Human S100B Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1820). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: S100B

S100B belongs to the S100 subgroup of the EF-hand family of Ca²⁺ binding proteins. It is a homodimer that is expressed primarily in the brain by astrocytes, oligodendrocytes and Schwann cells. S100B has multiple intracellular functions, but can also be secreted from cells to exert extracellular functions. Some of the extracellular functions of S100B may be mediated by RAGE (receptor for advanced glycation end products). Blood levels of S100B can be used to monitor the extent of brain injury and malignant melanoma.

Long Name

S100 Calcium Binding Protein B

Entrez Gene IDs

6285 (Human)

Alternate Names

beta (neural); NEF; S100 beta; S100 calcium binding protein B; S100 calcium-binding protein B; S100 calcium-binding protein, beta (neural); S-100 calcium-binding protein, beta chain, 10protein S100-B; S-100 protein beta chain; S-100 protein subunit beta; S100; S100B; S100beta

Product Datasheets

Product Datasheet

COA

SDS

Related Research Areas

Citations for Human S100B Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

6 Citations: Showing 1 - 6
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Astrocyte-derived MFG-E8 facilitates microglial synapse elimination in Alzheimer's disease mouse models
Authors: Sokolova, D;Ghansah, SA;Puletti, F;Georgiades, T;De Schepper, S;Zheng, Y;Crowley, G;Wu, L;Rueda-Carrasco, J;Koutsiouroumpa, A;Muckett, P;Freeman, OJ;Khakh, BS;Hong, S;
bioRxiv : the preprint server for biology
Species: Human
Sample Types: Whole Tissue
Applications: Immunohistochemistry
Zinc finger and SCAN domain-containing 18 suppresses the proliferation, self-renewal, and drug resistance of glioblastoma cells
Authors: Wang, Y;Peng, J;Song, C;Yang, Y;Qin, T;
Heliyon
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot
PLD3 affects axonal spheroids and network defects in Alzheimer's disease
Authors: P Yuan, M Zhang, L Tong, TM Morse, RA McDougal, H Ding, D Chan, Y Cai, J Grutzendle
Nature, 2022-11-30;612(7939):328-337.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC
Disease Progression-Dependent Effects of TREM2 Deficiency in a Mouse Model of Alzheimer's Disease.
Authors: Jay T, Hirsch A, Broihier M, Miller C, Neilson L, Ransohoff R, Lamb B, Landreth G
J Neurosci, 2017-01-18;37(3):637-647.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC
Increased serum S100B levels in chronic schizophrenic patients on long-term clozapine or typical antipsychotics.
Authors: Qi LY, Xiu MH, Chen da C, Wang F, Kosten TA, Kosten TR, Zhang XY
Neurosci. Lett., 2009-06-17;462(2):113-7.
Species: Human
Sample Types: Serum
Applications: ELISA Development
Glial Cell Line-derived Neurotrophic Factor-overexpressing Human Neural Stem/Progenitor Cells Enhance Therapeutic Efficiency in Rat with Traumatic Spinal Cord Injury
Authors: Kyujin Hwang, Kwangsoo Jung, Il-Sun Kim, Miri Kim, Jungho Han, Joohee Lim et al.
Experimental Neurobiology