LI-COR Data Examples for Mouse XL Cytokine Array

Data Examples

Profiling Proteins in Cell Culture Supernates

Figure 1. Balb/3T3 untreated vs. TNF-alpha; Treated Balb/3T3 mouse embryonic fibroblast cells were untreated or treated with 100 ng/mL recombinant mouse TNF-alpha; (R&D Systems, Catalog # 410-MT) for 24 hours. For each array shown, 500 µL of cell supernate was used. Array images were collected and analyzed using the LI-COR Odyssey Infrared Imaging System.

 

Figure 2. WEHI-3 untreated vs. LPS Treated WEHI-3 myelomonocytic leukemia cells were untreated or treated with 100 ng/mL LPS for 24 hours. For each array shown, 500 µL of cell supernate was used. Array images were collected and analyzed using the
LI-COR Odyssey Infrared Imaging System.

 

 

Figure 3. BV-2 untreated vs. LPS Treated BV-2 mouse microglial cells were untreated or treated with 100 ng/mL LPS for 4 or 24 hours. For each array shown, 200 µL of cell supernate was used. Array images were collected and analyzed using the
LI-COR Odyssey Infrared Imaging System.

 

 

Profiling Proteins in Cell Lysates

 

Figure 4. CMT-93 Untreated vs. LPS Treated Lysates from CMT-93 mouse rectal carcinoma cells were untreated or treated with 100 ng/mL LPS for 24 hours. 200 µg of cell lysate was run on each array. Array images were collected and analyzed using the
LI-COR Odyssey Infrared Imaging System.

 

Profiling Proteins In Body Fluids & Tissue Lysates

 

1 Adiponectin/Acrp30
2 LDL R
3 MMP-2
4 VEGF
5 Complement Factor D
6 EGF
7 Osteopontin (OPN)
8 TIM-1/KIM-1/HAVCR
9 Chitinase 3-like 1
10 Osteopontin
11 FGF acidic
12 RAGE
13 CCL6/C10
14 CCL11/Eotaxin
15 IGFBP-6
16 Resistin
17 BAFF/BLyS/TNFSF13B
18 CD40/TNFRSF5
19 Endostatin
20 P-Selection/CD62P

Figure 5. The Mouse XL Cytokine Array detects multiple analytes in serum, urine, lung tissue lysate, uterus tissue lysate and spleen tissue lysate samples.

The sample type and quantity used per array is listed. Array images were collected and analyzed using the LI-COR Odyssey Infrared Imaging System.

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